流程包括:fastqc测序原始数据、fastp质控、hisat2比对、stringtie拼接、kallisto定量等
文献引用:
1
Chen S , Zhou Y , Chen Y , et al. fastp: an ultra-fast all-in-one FASTQ preprocessor[J]. Bioinformatics, 2018, 34(17):i884-i890.
2
Li H , Handsaker B , Wysoker A , et al. The Sequence Alignment/Map format and SAMtools[J]. Bioinformatics, 2009, 25(16):2078-2079.