Active packaging that prolongs food shelf life, maintaining its quality and safety, is an increasing industrial demand, especially if integrated in a circular economy model. In this study, the fabrication and characterization of sustainable cellulose-based active packaging using food-industry waste and natural extracts as antioxidant agents was assessed. Grape marc, olive pomace and moringa leaf extracts obtained by supercritical fluid, antisolvent and maceration extraction in different solvents were compared for their antioxidant power and phenolic content. Grape and moringa macerates in acetone and methanol, as the most efficient and cost-effective extracts, were incorporated in the packaging as coatings or in-between layers. Both systems showed significant free-radical protection in vitro (antioxidant power 50%) and more than 50% prevention of ground beef lipid peroxidation over 16 days by indirect TBARS and direct in situ Raman microspectroscopy measurements. Therefore, these systems are promising for industrial applications and more sustainable farm-to-fork food production systems.

High pressure processing (HPP) was found to have a kinase effect on persimmon pectin methyl esterase (PME), while the mechanism remains unclear. In this study, chemical experiments and molecular dynamics (MD) simulations were used to reveal its mechanisms. Persimmon PME was first extracted and purified using ion exchange columns with 81.89% purity. After 500 MPa/5 min, PME activity increased 11.3%, the α-helix and β-folding decreased 10.8% and 6.1% compared to the 0.1 MPa group, respectively. MD results showed that HPP decreased the volume, increased the number of hydrogen bonds between PME and pectin. Under high pressure, Asp-157, Asp-136 and Gln-135 in the enzyme activity center remained stable, while the positions of Arg-225 and Gln-113 changed a lot. The conformation of the substrate binding channel also changed. The secondary structure and volume changes of the HPP-treated PME affected the active center and substrate channels, ultimately altering the activity.

Herein, an efficient, simple and economical approach to remove antibiotics (ABX), i.e. ceftiofur hydrochloride, sulfamonomethoxine sodium (SMM), marbofloxacin and oxytetracycline by sonication with activated carbon (AC) from cow milk has been successfully implemented. The pseudo-first-order kinetics constants for the sonolytic and sonocatalytic degrading SMM are 0.036 and 0.093 min-1, respectively. The synergistic efficiency of removing ABX by using sonocatalysis reached 1.8-4.0. Hydrophobic ABX underwent faster degradation than hydrophilic ABX in sonocatalytic systems. Adding 0.5 mmol L-1 H2O2, the optimal concentration, improved the sonocatalytic degradation rates of ABX by 9.1%-28.5%. Surface area and dose of AC play crucial roles in the sonocatalysis of ABX. By sonicating 50 mL of 5.52 μmol L-1 ABX in milk at 500 kHz and 259 W with 20 mg AC for 20-60 min resulted in residual ABX concentrations ranging from 42.6 to 95.1 μg L-1, which meet the relative maximum residue limits set by European Commission.

Recent advances in 3D printing technology have provided a new avenue for food manufacturing. However, one challenge in 3D printing food is the limited availability of printable materials that can mimic the properties of real food. This review focused on the various 3DFP methodologies, as well as the reinforcement of natural food gel for improving printing features in 3D printed food. Also covered is the use of hydrogel-based 3D printing in the development of 3D printed food. Different 3D printing techniques can be employed to print hydrogel-based inks, each with its advantages and limitations. 3D printing of food using hydrogel-based inks has potential for customized food products development. In summary, the utilization of hydrogel-based inks in 3D printing offers a promising avenue for the development of customized food products. Although there are still challenges to overcome, such as improving the printability and mechanical properties of hydrogel-based inks, the potential benefits of this technology make it an exciting area of research.

In this study, gold nanoparticles decorated on nickel-iron layered double hydroxide with black phosphorus nanosheets (AuNP/Ni-Fe LDH/BPNSs) composite were prepared using a stirring method. Analyte tracing is required for developing viable sensors. The AuNP/Ni-Fe LDH/BPNSs composite exhibited a large specific surface area, high conductivity, high electrocatalytic activity, and rapid electron transfer. These properties play a vital role in monitoring diphenylamine (DPA) in food samples. The formation of the AuNP/Ni-Fe LDH/BPNSs composite was confirmed using various structural and morphological characterization techniques. The electroanalytical character of the AuNP/Ni-Fe LDH/BPNSs composite was evaluated using voltammetry. Interestingly, the AuNP/Ni-Fe LDH/BPNSs showed a wide linear range of 0.0125-1003.82 μM and a detection limit of 4.63 nM with a sensitivity of 0.399 µA µM-1 cm-2. The constructed sensor shows considerable selectivity, stability, repeatability, and reproducibility, and the practicability of DPA was monitored in the apples, sweet tomatoes, pears, and grapes with satisfactory recoveries.

A co-fermentation strategy was explored to rapidly improve the characteristic components and quality of Pericarpium Citri Reticulatae (PCR) using Monascus anka and Saccharomyces cerevisiae, and the enzymatic mechanism was investigated. The results showed that the free flavonoid content of fermented PCR was 48.12% higher than that of unfermented PCR after 12 days of co-fermentation, resulting in stronger antioxidant activity. d-Limonene, γ-terpinene, proline (Pro), arginine (Arg), and serine (Ser) contributed the most to the flavors of citrus, herb, and sweet citrus based on odor and taste activity value analysis. Metabolomics and multivariate statistics showed that 55 components were differentially metabolized during co-fermentation, and ten metabolic pathways were closely related to metabolism. Furthermore, five hydrolases participated in the release and conversion of the active ingredients. This study provides an effective processing method for PCR and is conducive to the development of new PCR functional health foods.

Potassium ferricyanide (K4[Fe(CN)6]) as anti-caking agent plays an important role in avoiding the formation of chunks for fine particulate solids. However, inappropriate and excessive addition and decomposition of K4[Fe(CN)6] are detrimental to physical health. At present, appropriate strategies for convenient and accurate analysis of K4[Fe(CN)6] in table salt and pickled food are desirable. Herein, an efficient "ON-OFF-ON" fluorescent sensor based on chiral carbon dots was prepared by a simple one-step hydrothermal method. The chiral CDs with L-Tryptophan and D-Tryptophan as chiral source were named as L-CDs and D-CDs. Notably, the bright fluorescence of L/D-CDs could be effectively quenched by K4[Fe(CN)6] through dynamic quenching mechanism. This fluorescent sensor achieved excellent sensitive and selective detection of K4[Fe(CN)6] with a limit of detection (LOD) of 25.0 ng·mL-1. In addition, the L/D-CDs could be applied not only for selective fluorescent recognition of K4[Fe(CN)6] by the methods of portable filter paper and hydrogels, but also as fluorescent dye for repeated message encryption and decryption.

This study aimed to fabricate novel nanocomplexes for delivery of anthocyanins (ACN) utilizing ovalbumin (OVA) and sulphated-polysaccharides with varying linear charge density (κ-,ι-, λ-carrageenan and dextran sulfate: κC < ιC < λC < DS). Influence of OVA-sulphated-polysaccharides on ACN stability, antioxidant capacity, and bioaccessibility was investigated. Fabricated nanoparticlecosmeticsed superior encapsulation efficiency (94.11-96.2%) and loaded capacity (9.05-9.54%) for ACN. OVA-DS displayed the smallest particle size and turbidity, while OVA-κC-ACN exhibited the largest ones. ζ-Potential of nanoparticles raised with increasing ester-sulfate level in sulphated-polysaccharides. FT-IR, Raman and OVA conformational alterations revealed existence of intermolecular-interactions between ACN and OVA-polysaccharides. DSC and TGA showed considerable thermo-stability of self-assembled (ACN-loaded) OVA-polysaccharides. Spheroid-nanoparticles size increased after ACN-loading in SEM and CLSM. Composite nanocomplexes enhanced ACN stability and antioxidant properties under accelerated degradation conditions and simulated digestion, particularly, OVA-DS-ACN and OVA-λC-ACN. We provide a choice for reinforcing stability of hydrophilic nutraceuticals and improving its applications.

Currently, the effect of varieties on the flavor and stability of flaxseed milk remains unknown. Therefore, this study was conducted to investigate the effects of different varieties on the stability, sensory, and aromas of flaxseed milk. 51 volatile compounds were identified in flaxseed milk using Stir Bar Sorptive Extraction-Gas Chromatography-Olfactometry-Mass Spectrometry (SBSE-GC-O-MS). Among them, 1-octen-3-ol, 2-methoxy-4-vinylphenol, and 2,3,5-trimethylpyrazine contributed higher relative odor active values (ROAV), resulted in the fruity, roasted, sweet, and cucumber in flaxseed milk. Isovaleraldehyde (green notes) was not detected in XHZM. However, other compounds such as 1-nonanol (floral), γ-nonanolactone and γ-octanoic lactone (coconut milk) had higher concentrations, causing a better sensory evaluation. Additionally, its stability was relatively good. The orthogonal partial least-squares regression (OPLS) model and VIP values showed that eight compounds were responsible for the sensory differences from different varietals. The study provided references to selection and understanding flavor composition basis of flaxseed milk.

Herein, new pre-column derivatization based on dansylation is present to resolve analytical difficulties, such as chromatographic separation difficulty, in identifying and quantifying advanced glycation end products (AGEs) owing to their high hydrophilicity, wide variety, and structural similarity. The proposed analytical method facilitated the separation of 14 AGEs, including structural isomers. Limits of detection of 1.0-43.3 ng/mL and linear ranges of the double- or triple-digit were achieved. Intra- and inter-day precisions of 1.1-3.0% and 1.3-3.1%, respectively, were achieved for standard solutions, while those for food specimens were 1.4-11.2% and 1.7-15.7%, respectively. The matrix effect was insignificant with regard to the percent recoveries and differences between slopes for both the standard solutions and food specimens. Furthermore, the quantitation results of AGEs in foods (coffee, beer, and sausage) and glycated proteins revealed the potential applicability of the developed method in various fields of food chemistry and biochemistry.

This work investigated the effects of liquid nitrogen immersion freezing (LNF), -35 °C air freezing (AF-35℃) and -18 °C air freezing (AF-18℃) on the physical and chemical characteristics and flavor quality of surimi gels with different cross-linking degrees. Compared to AF-35 °C and AF-18 °C, LNF was shown to considerably delay the texture deterioration and water migration of frozen gels, as well as the accumulation of thiobarbituric acid reactive substance values and carbonyl contents. Additionally, an appropriate increase of cross-linking degree (45.83 to 62.99%) was found able to improve gel properties and inhibit quality deterioration during freezing. Moreover, LNF-treated gels were closer to fresh gels in the amount of volatile compounds, in contrast to most significant negative aroma changes in AF-18℃-treated gels. Furthermore, 29, 29 and 31 key differential volatile compounds were screened for gels with a cross-linking degree of 29.66, 45.83 and 62.99%, respectively, mainly including aldehydes, alcohols and esters.

This study was aimed to extract bioactive peptides from the white and purple flower varieties of Orthosiphon aristatus leaves. The herb is well known for its pharmacological importance, possibly attributed to its plant proteins. Phenol based extraction was used to extract plant proteins, and then hydrolysed by proteolytic enzymes such as trypsin (serine protease) and pepsin (aspartic protease). MS/MS analysis revealed that 145 and 125 proteins were detected from the white and purple flower varieties, respectively. Trypsin hydrolysates were showed to have a higher degree of hydrolysis (24-33%), resulting in higher antioxidant and antibacterial activities. The white flower of trypsin hydrolysates showed a higher radical scavenging activity which could be attributed to its higher content of stress proteins (19%). However, trypsin hydrolysates from the purple flower showed higher ferric reducing power and bacterial growth inhibition. The performance of hydrolysates was better than ampicillin in inhibiting Acinetobacter baumanni and Staphylococcus aureus.

Melamine added to milk powder can lead to kidney injury and even death, but rapid detection is still hard due to the strong interference of milk powder solution. Herein, the CC/CeO2/CNPs mesh was constructed to detect melamine by using the photo-enhanced barrier effects on electrochemical response. Schottky barrier was regulated effectively to produce electrochemical response to melamine by photo-induced electrostatic interaction, which exhibited strong resistance to interference in milk powder solution. Sensitivity was enhanced by nearly 5 times and the lowest detection limit was reduced as low as 0.274 nM. The obtained high recovery (100%-104%) and good stability in milk powder solution indicated the good potential for practical applications. It provides a new opportunity for achieving strong resistance to interference by using the photo-enhanced barrier driving effect on electrochemical response.

Gastric digestion conditions change during infancy from newborn towards more adult digestion conditions, which can change gastric digestion kinetics. However, how these changes in gastric digestion conditions during infancy affect milk protein digestion has not been investigated. Therefore, we aimed to investigate milk protein digestion with static in vitro gastric digestion models representing one-, three- and six-month-old infants. With increasing age, gastric clots and soluble proteins were digested more extensively, which may partly be attributed to the looser gastric clot structure. Larger differences with increasing age were found for heated than unheated milk proteins, which might be caused by the presence of denatured whey proteins. Taken together, these findings show that gastric milk protein digestion increases during infancy. These in vitro gastric digestion models could be used to study how milk protein digestion changes with infant age, which may aid in developing infant formulas for different age stages.

Developing rapid monitoring methods to detect antibiotic residues in food plays an important role in safeguarding human health. This study presents the development of a novel fluorescence-enhanced detection method for chlortetracycline (CTC) using a GSH-Se nanozyme. A GSH-Se nanozyme prepared using a one-pot hydrothermal method not only possesses excellent fluorescent properties but also exhibits good glutathione peroxidase-like activity. The results show that the addition of CTC leads to a significant enhancement in the fluorescence intensity of GSH-Se, and this increase exhibits a good linear relationship with the concentration of CTC. The linear range of this method is 0.02-1 µM, and the limit of detection (LOD) for CTC was 0.02 µM. Moreover, the cell toxicity of GSH-Se is low and can be used for monitoring and imaging of CTC in cells, and satisfactory results have been obtained in the analysis of actual food samples.

Flavor characteristics of Shanlan rice wines with different fermentation time were analyzed. Results suggested that 3-methyl-1-butanol, phenylethyl alcohol, ethyl acetate, and diethyl succinate were the characteristic volatiles of Shanlan rice wine by using headspace solid-phase microextraction-gas chromatography-mass spectrometry-olfactometry. The most varieties (38) of volatiles appeared at a 3-year-fermentation time, contributing a unique and harmonious aroma to the Shanlan rice wine fermented for 3 years, but only 19 types were observed at 45-days and 1-year fermentation times. A similar trend was intuitively visible in the headspace-gas chromatography-ion mobility spectrometry analysis. The Shanlan rice wine fermented for 3 years had a similar taste profile to that fermented for 45 days, but with distinguishing contents of free amino acids (1352.80 mg/L and 2261.50 mg/L, respectively) and organic acids (9.58 g/L and 49.88 g/L, respectively). The Shanlan rice wine fermented for 1 year had a strong taste with more intensity of most taste attributes.

Vitamin E consists of four (α-, β-, γ-, δ-) isoforms of tocopherols (T) and tocotrienols (T3), collectively known as tocols. Current LC methods for tocols suffer from either the poor ability to resolve the β- and γ- isoforms (RPLC), or require the use of nonpolar solvents (NPLC), which complicates subsequent MS/MS detection. Moreover, we show that coupling of UV with MS leads to tocols photodegradation. To solve these problems, we developed a new RPLC-MS/MS method, allowing to resolve not only α- and δ-, but also β- and γ- tocols in hydrophobic matrices. We took advantage of an observation that the peak area ratios are specific for the given isomer and constant. The new method with a linear range between 0.2 and 60 ng·mL-1 (for α-T) and 1.1-60 ng·mL-1 (for β-T3 and γ-T3) was validated and employed for quantitative analysis of several oils, including false flax (Camelina sativa) oil stored under different conditions.

The aroma produced during drying is an important indicator of tencha and needs to be monitored. This study constructed an olfactory visualization system for assessing tencha aroma using colorimetric sensor array (CSA) combined with chemometric methods. The 16 chemically responsive dyes were selected to obtain aroma information of tencha samples and extracted image data of aroma information by machine vision algorithms. Subsequently, k-nearest neighbor, support vector machine, classification and regression tree, and random forest (RF), four qualitative models were applied to build the mathematical models. The RF model with nine principal components was preferred, with recognition rate of 100.00% and 91.07% for the training and prediction sets, respectively. The experimental results showed that CSA combined with the RF model can be effectively applied to assess tencha aroma. This study provided a scientific and novel method to maintain the stability of tencha quality in the production process.

In the booming global population, monitoring of mesotrione (MTN) like agricultural pollutants is crucial for human safety. Herein, the research reports the synthesis of tungsten trioxide (WO3) and cobalt oxide (Co3O4) nanostructures intercalated ball milling exfoliated graphene (WO3/Co3O4/graphene) 3D helix electrocatalyst for the electrochemical detection of MTN herbicide. The proposed WO3/Co3O4/graphene sensor material achieved a wide range of MTN detection from 0.001 µM to 1885 µM. In addition, the estimated limit of sensing and sensitivity values are 0.42 nM and 0.802 µAµM-1 cm-2 respectively. The real sample experiment was accomplished in MTN-added vegetables (corn, sugar cane, tomato, green soybean) and environmental samples (sewage water, river water). At most, the recorded minimum MTN response recovery in vegetables and water samples is about 95% and 98% respectively. Furthermore, the designed sensor electrode achieved storage stability of 98.7% after three weeks.

Ochratoxin A (OTA) is a toxic pollutant in foods, and its actual detection is crucial. A novel and sensitive dual signaling ratiometric electrochemical aptasensor based on nanoporous gold (NPG) was proposed to determine OTA. NPG, with high specific surface area and conductivity, improved the sensitivity by immobilizing more aptamers. Meanwhile, the dual signaling ratiometric strategy improved the detection reproducibility through self-referencing and built-in correction. NPG and ratiometric strategy multi-amplified the dual signal changes. The sensitivity of OTA was evaluated by the ratio of methylene to ferrocene current values. Under the optimal conditions, the NPG-based aptasensor demonstrated excellent sensitivity with a wide linear range of 1 pg/mL to 2 ng/mL and the limit of detection (LOD) of 0.4 pg/mL for OTA. This developed aptasensor also effectively detected OTA in spiked Cordyceps sinensis and grape juice samples, with recovery values falling in the 98.49-108.0% range.