For non-target residue analysis of xenoestrogens in food, sophisticated chromatographic-mass spectrometric techniques lack in biological effect detection. Various in vitro assays providing sum values encounter problems when opposing signals are present in a complex sample. Due to physicochemical signal reduction, cytotoxic or antagonistic effect responses, the resulting sum value is falsified. Instead, the demonstrated non-target estrogenic screening with an integrated planar chromatographic separation differentiated opposing signals, detected and prioritized important estrogenic compounds, and directly assigned tentatively the responsible compounds. Sixty pesticides were investigated, ten of which showed estrogenic effects. Exemplarily, half-maximal effective concentrations and 17β-estradiol equivalents were determined. Estrogenic pesticide responses were confirmed in six tested plant protection products. In food, such as tomato, grape, and wine, several compounds with an estrogenic effect were detected. It showed that rinsing with water was not sufficient to remove selected residues and illustrated that, though not usually performed for tomatoes, peeling would be more appropriate. Though not in the focus, reaction or breakdown products that are estrogenic were detected, underlining the great potential of non-target planar chromatographic bioassay screening for food safety and food control.

Current strategies for non-target food screening focus mainly on known hazardous chemicals (adulterants, residues, contaminants, packaging migrants, etc.) instead of bioactive constituents in general and exclude the biological effect detection. To widen the perspective, a more proactive non-target effect-directed strategy is introduced to complement food safety in order to detect not only known but also unknown bioactive compounds. The developed 10-dimensional hyphenation included on-surface digestion (1D), planar chromatographic separation (2D), visualization using white light (3D), UV light (4D), fluorescence light (5D), effect-directed assay analysis (6D), heart-cut zone elution to an orthogonal reversed phase column chromatography including online desalting (7D) with subsequent diode array detection (8D), high-resolution mass spectrometry (9D), and fragmentation (10D). Metabolism, i.e., intestinal digestion of each sample, was simulated and integrated on the same adsorbent surface to study any changes in the compound profiles. As proof of principle, nine convenience tomato products and a freshly prepared tomato soup were screened via five different planar assays in a non-targeted mode. Non-digested and digested samples were compared side by side. In their effect-directed profiles, 14 bioactive compounds from classes of lipids, plant hormones, spices, and pesticides were identified. In particular, bioactive compounds coming from the lipid class were increased by gastrointestinal digestion, while spices and pesticides remained unaffected. With regard to food safety, the determination of the two dinitrophenol herbicides dinoterb and dinoseb in highly processed tomato products should be given special attention. The hyphenation covered a broad analyte spectrum and showed robust and reliable results.