Grasslands are the most dominant terrestrial ecosystem in China, but few national grassland maps have been generated. The grassland resource map produced in the 1980s is widely used as background data, but it has not been updated for almost 40 years. Therefore, a reliable map depicting the current spatial distribution of grasslands across the country is urgently needed. In this study, we evaluated the grassland consistency and accuracy of ten land cover datasets (GLC2000, GlobCover, CCI-LC, MCD12Q1, CLUD, GlobeLand30, GLC-FCS30, CGLS-LC100, CLCD, and FROM-GLC) for 2000, 2010, and 2020 based on extensive fieldwork. We concluded that the area of these ten grassland products ranges from 107.80×104 to 332.46×104 km2, with CLCD and MCD12Q1 having the highest area consistency. The spatial and sample consistency is highest in the regions of east-central Inner Mongolia, the Qinghai-Tibet Plateau and northern Xinjiang, while the distribution of southern grasslands is scattered and differs considerably among the ten products. MCD12Q1 is significantly more accurate than the other nine products, with an overall accuracy (OA) reaching 77.51% and a kappa coefficient of 0.51; CLCD is slightly less accurate than MCD12Q1 (OA=73.02%, kappa coefficient=0.45) and is more conducive to the fine monitoring and management of grassland because of its 30-meter resolution. The highest accuracy of grassland was found in the Inner Mongolia-Ningxia region and Qinghai-Tibet Plateau, while the accuracy was worst in the southeastern region. In the future grassland mapping, cartographers should improve the accuracy of the grassland distribution in South China and regions where grassland is confused with forest, cropland and bare land. We specify the availability of valuable data in existing land cover datasets for China's grasslands and call for researchers and the government to actively produce a new generation of grassland maps.

Soybean is a leguminous crop that provides oil and protein. Exploring the genomic signatures of soybean evolution is crucial for breeding varieties with improved adaptability to environmental extremes. We analyzed the genome sequences of 2,214 soybeans and proposed a soybean evolutionary route, i.e., the expansion of annual wild soybean (Glycine soja Sieb. & Zucc.) from southern China and its domestication in central China, followed by the expansion and local breeding selection of its landraces (G. max (L.) Merr.). We observed that the genetic introgression in soybean landraces was mostly derived from sympatric rather than allopatric wild populations during the geographic expansion. Soybean expansion and breeding were accompanied by the positive selection of flowering time genes, including GmSPA3c. Our study sheds light on the evolutionary history of soybean and provides valuable genetic resources for its future breeding.

Golden2 (G2), a member of the GARP transcription factor superfamily, regulates several biological processes and phytohormone signaling pathways in plants. In this study, we used a rice codon-optimized maize G2 gene (rZmG2) to improve the regeneration efficiency of rice and maize calli for genetic transformation. We isolated a promoter driving strong and callus-specific expression from rice to drive rZmG2 transcription from a transgene after transformation of two indica and two japonica rice cultivars. The resulting rZmG2 transgenic calli turned green in advance at the differentiation stage, thus significantly raising the regeneration rates of the transgenic indica and japonica rice plants relative to control transformations. Similar effect of this gene on improving maize transformation was also observed. Transcriptome sequencing and RT-qPCR analyses showed that many rice genes related to chloroplast development and phytohormones are upregulated in rZmG2-transgenic calli. These results demonstrate that rZmG2 can promote embryogenic callus differentiation and improve regeneration efficiency by activating chloroplast development and phytohormone pathways. We also established a heat-inducible Cre/loxP-based gene-excision system to remove rZmG2 and the antibiotic selectable gene after obtaining the transgenic plants. This study provides a useful tool for functional genomics work and biotechnology in plants.