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Biomolecules

2022 Sep 9

Li HY, Yang WQ, Zhou XZ

2. Phosphatidylethanolamine N-Methyltransferase Knockout Modulates Metabolic Changes in Aging Mice.

Biomolecules

2022 Sep 9

Zhou Q, Zhang F, Kerbl-Knapp J

3. eIF3 Interacts with Selenoprotein mRNAs.

Biomolecules

2022 Sep 9

Hayek H, Eriani G, Allmang C.

4. Amaryllidaceae Alkaloids Decrease the Proliferation, Invasion, and Secretion of Clinically Relevant Cytokines by Cultured Human Colon Cancer Cells.

Biomolecules

2022 Sep 9

Mathieu V, Laguera B, Masi M

5. The Role of Intrinsically Disordered Proteins in Liquid-Liquid Phase Separation during Calcium Carbonate Biomineralization.

Biomolecules

2022 Sep 9

Tarczewska A, Bielak K, Zoglowek A

6. The Ni(II)-Binding Activity of the Intrinsically Disordered Region of Human NDRG1, a Protein Involved in Cancer Development.

Biomolecules

2022 Sep 9

Beniamino Y, Cenni V, Piccioli M

7. Simulated Docking Predicts Putative Channels for the Transport of Long-Chain Fatty Acids in Vibrio cholerae.

Biomolecules

2022 Sep 9

Turgeson A, Morley L, Giles D

8. Molecular Determinants Underlying the Anti-Cancer Efficacy of CD38 Monoclonal Antibodies in Hematological Malignancies.

Biomolecules

2022 Sep 8

Mustafa N, Azaman MI, Ng GGK

9. Rapid Assessment of Lipidomics Sample Purity and Quantity Using Fourier-Transform Infrared Spectroscopy.

Biomolecules

2022 Sep 8

Robinson H, Molendijk J, Shah AK

Despite the increasing popularity of liquid chromatography-mass spectrometry (LC-MS)-based lipidomics, there is a lack of accepted and validated methods for lipid extract quality and quantity assessment prior to LC-MS. Fourier-Transform Infrared Spectroscopy (FTIR) has been reported for quantification of pure lipids. However, the impact of complex lipid sample complexity and purity on total lipid quantification accuracy has not been investigated. Here, we report comprehensive assessment of the sample matrix on the accuracy of lipid quantification using Attenuated Total Reflectance (ATR)-FTIR and establish a simple workflow for lipidomics sample quantification. We show that both pure and complex lipids show characteristic FTIR vibrations of CH- and C=O-stretching vibrations, with a quantitative range of 40-3000 ng and a limit of detection of 12 ng, but sample extraction method and local baseline subtraction during FTIR spectral processing significantly impact lipid quantification via CH stretching. To facilitate sample quality screening, we developed the Lipid Quality (LiQ) score from a spectral library of common contaminants, using a ratio of peak heights between CH stretching vibrations maxima and the collective vibrations from amide/amine, CH-stretching minima and sugar moieties. Taking all tested parameters together, we propose a rapid FTIR workflow for routine lipidomics sample quality and quantity assessment and tested this workflow by comparing to the total LC-MS intensity of targeted lipidomics of 107 human plasma lipid extracts. Exclusion of poor-quality samples based on LiQ score improved the correlation between FTIR and LC-MS quantification. The uncertainty of absolute quantification by FTIR was estimated using a 795 ng SPLASH LipidoMix standard to be <10%. With low sample requirement, we anticipate this simple and rapid method will enhance lipidomics workflow by enabling accurate total lipid quantification and normalization of lipid quantity for MS analysis.

10. Mucus-Penetrating Silk Fibroin-Based Nanotherapeutics for Efficient Treatment of Ulcerative Colitis.

Biomolecules

2022 Sep 8

Xie D, Zhou X, Xiao B

11. Functional Identification of Arthrinium phaeospermum Effectors Related to Bambusa pervariabilis × Dendrocalamopsis grandis Shoot Blight.

Biomolecules

2022 Sep 8

Fang X, Yan P, Luo F

12. The Allopregnanolone Response to Acute Stress in Females: Preclinical and Clinical Studies.

Biomolecules

2022 Sep 8

Pisu MG, Concas L, Siddi C

13. Public Transcriptomic Data Meta-Analysis Demonstrates TAAR6 Expression in the Mental Disorder-Related Brain Areas in Human and Mouse Brain.

Biomolecules

2022 Sep 7

Vaganova AN, Katolikova NV, Murtazina RZ

14. Recent Advances of Degradation Technologies Based on PROTAC Mechanism.

Biomolecules

2022 Sep 7

Xiao M, Zhao J, Wang Q

15. Where Electrostatics Matter: Bacterial Surface Neutralization and Membrane Disruption by Antimicrobial Peptides SAAP-148 and OP-145.

Biomolecules

2022 Sep 7

Vejzovic D, Piller P, Cordfunke RA

16. Proinflammatory and Cancer-Promoting Pathobiont Fusobacterium nucleatum Directly Targets Colorectal Cancer Stem Cells.

Biomolecules

2022 Sep 7

Cavallucci V, Palucci I, Fidaleo M

17. Advances in the Application of Electrospun Drug-Loaded Nanofibers in the Treatment of Oral Ulcers.

Biomolecules

2022 Sep 7

Zhou Y, Wang M, Yan C

18. SARS-CoV-2 Invasion and Pathological Links to Prion Disease.

Biomolecules

2022 Sep 7

Lukiw WJ, Jaber VR, Pogue AI

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of the COVID-19 disease, is a highly infectious and transmissible viral pathogen that continues to impact human health globally. Nearly ~600 million people have been infected with SARS-CoV-2, and about half exhibit some degree of continuing health complication, generically referred to as long COVID. Lingering and often serious neurological problems for patients in the post-COVID-19 recovery period include brain fog, behavioral changes, confusion, delirium, deficits in intellect, cognition and memory issues, loss of balance and coordination, problems with vision, visual processing and hallucinations, encephalopathy, encephalitis, neurovascular or cerebrovascular insufficiency, and/or impaired consciousness. Depending upon the patient's age at the onset of COVID-19 and other factors, up to ~35% of all elderly COVID-19 patients develop a mild-to-severe encephalopathy due to complications arising from a SARS-CoV-2-induced cytokine storm and a surge in cytokine-mediated pro-inflammatory and immune signaling. In fact, this cytokine storm syndrome:&nbsp;(i) appears to predispose aged COVID-19 patients to the development of other neurological complications, especially those who have experienced a more serious grade of COVID-19 infection; (ii) lies along highly interactive and pathological pathways involving SARS-CoV-2 infection that promotes the parallel development and/or intensification of progressive and often lethal neurological conditions, and (iii) is strongly associated with the symptomology, onset, and development of human prion disease (PrD) and other insidious and incurable neurological syndromes. This commentary paper will evaluate some recent peer-reviewed studies in this intriguing area of human SARS-CoV-2-associated neuropathology and will assess how chronic, viral-mediated changes to the brain and CNS contribute to cognitive decline in PrD and other progressive, age-related neurodegenerative disorders.

19. Fatty Acids: Essential Nutrients and Important Biomarkers.

Biomolecules

2022 Sep 7

Gladyshev MI.

20. Expression and Function of ABC Transporters in Human Alveolar Epithelial Cells.

Biomolecules

2022 Sep 7

Visigalli R, Rotoli BM, Ferrari F

21. INTERCEPT Pathogen Reduction in Platelet Concentrates, in Contrast to Gamma Irradiation, Induces the Formation of trans-Arachidonic Acids and Affects Eicosanoid Release during Storage.

Biomolecules

2022 Sep 7

Leitner GC, Hagn G, Niederstaetter L

22. Galectins: Their Network and Roles in Infection/Immunity/Tumor Growth Control 2021.

Biomolecules

2022 Sep 7

Hattori T.

23. Gut Microbiota Regulation of AHR Signaling in Liver Disease.

Biomolecules

2022 Sep 6

Wang B, Zhou Z, Li L.

24. MiR-223 and MiR-186 Are Associated with Long-Term Mortality after Myocardial Infarction.

Biomolecules

2022 Sep 6

Elbaz M, Faccini J, Laperche C

25. Marine Natural Compounds with Biomedical Potential.

Biomolecules

2022 Sep 6

Leychenko E.

26. Development of Biodegradable Polymeric Stents for the Treatment of Cardiovascular Diseases.

Biomolecules

2022 Sep 6

Shen Y, Yu X, Cui J

27. Natural and Modified Oligonucleotide Sequences Show Distinct Strand Displacement Kinetics and These Are Affected Further by Molecular Crowders.

Biomolecules

2022 Sep 6

Domljanovic I, Ianiro A, Rüegg C

28. Methyltransferases of Riboviria.

Biomolecules

2022 Sep 6

Mushegian A.

29. Protein Function Analysis through Machine Learning.

Biomolecules

2022 Sep 6

Avery C, Patterson J, Grear T

30. Iron in Alzheimer's Disease: From Physiology to Disease Disabilities.

Biomolecules

2022 Sep 6

Pal A, Cerchiaro G, Rani I

31. Research Progress on Nanoparticles-Based CRISPR/Cas9 System for Targeted Therapy of Tumors.

Biomolecules

2022 Sep 5

Nie D, Guo T, Yue M

32. Application of Metal-Organic Framework in Diagnosis and Treatment of Diabetes.

Biomolecules

2022 Sep 5

Gao Q, Bai Q, Zheng C

33. Peptidomimetics and Their Applications for Opioid Peptide Drug Discovery.

Biomolecules

2022 Sep 5

Lee YS.

34. 4-OI Protects MIN6 Cells from Oxidative Stress Injury by Reducing LDHA-Mediated ROS Generation.

Biomolecules

2022 Sep 4

Wu J, Gu X, Zhang J

35. Promising Roles of Circular RNAs as Biomarkers and Targets for Potential Diagnosis and Therapy of Tuberculosis.

Biomolecules

2022 Sep 4

Huang Y, Li Y, Lin W

36. The Key Elements for Biomolecules to Biomaterials and to Bioapplications.

Biomolecules

2022 Sep 4

Yu DG, Zhao P.

37. Three Grand Challenges in High Throughput Omics Technologies.

Biomolecules

2022 Sep 4

Suravajhala P, Goltsov A.

38. A New Product of Bilirubin Degradation by H(2)O(2) and Its Formation in Activated Neutrophils and in an Inflammatory Mouse Model.

Biomolecules

2022 Sep 4

Yu FF, Yuan Y, Ao Y

Bilirubin (BR) is a tetrapyrrolic compound stemming from heme catabolism with diverse physiological functions. It can be oxidized by H2O2 to form several degradation products, some of which have been detected in vivo and may contribute to the pathogenesis of certain diseases. However, the oxidative degradation of BR is complex and the conditions that BR degradation occurs pathophysiologically remain obscure. Neutrophils are known to generate large amounts of reactive oxygen species, including H2O2, upon activation and they are mobilized to inflammatory sites; therefore, we hypothesized that activated neutrophils could cause BR degradation, which could occur at inflammatory sites. In the present study, we investigated BR degradation by H2O2 and identified hematinic acid (BHP1) and a new product BHP2, whose structure was characterized as 2,5-diformyl-4-methyl-1H-pyrrole-3-propanoic acid. An LC-MS/MS method for the quantitation of the two compounds was then established. Using the LC-MS/MS method, we observed the concentration-dependent formation of BHP1 and BHP2 in mouse neutrophils incubated with 10 and 30 μM of BR with the yields being 16 ± 3.2 and 31 ± 5.9 pmol/106 cells for BHP1, and 25 ± 4.4 and 71 ± 26 pmol/106 cells for BHP2, respectively. After adding phorbol 12-myristate 13-acetate, a neutrophil agonist, to 30 μM of BR-treated cells, the BHP1 yield increased to 43 ± 6.6 pmol/106 cells, whereas the BHP2 one decreased to 47 ± 9.2 pmol/106 cells. The two products were also detected in hemorrhagic skins of mice with dermal inflammation and hemorrhage at levels of 4.5 ± 1.9 and 0.18 ± 0.10 nmol/g tissue, respectively, which were significantly higher than those in the non-hemorrhagic skins. BHP2 was neurotoxic starting at 0.10 μM but BHP1 was not, as assessed using Caenorhabditis elegans as the animal model. Neutrophil-mediated BR degradation may be a universally pathophysiological process in inflammation and can be particularly important under pathological conditions concerning hemorrhage.

39. Peripheral Neutrophil-to-Lymphocyte Ratio in Bronchiectasis: A Marker of Disease Severity.

Biomolecules

2022 Sep 30

Martinez-García MÁ, Olveira C, Girón R

Most patients with bronchiectasis have a predominantly neutrophilic inflammatory profile, although other cells such as lymphocytes (as controllers of bronchial inflammation) and eosinophils also play a significant pathophysiological role. Easy-to-interpret blood biomarkers with a discriminative capacity for severity or prognosis are needed. The objective of this study was to assess whether the peripheral neutrophil-to-lymphocyte ratio (NLR) is associated with different outcomes of severity in bronchiectasis. A total of 1369 patients with bronchiectasis from the Spanish Registry of Bronchiectasis were included. To compare groups, the sample was divided into increasing quartiles of NLR ratio. Correlations between quantitative variables were established using Pearson's P test. A simple linear regression (with the value of exacerbations as a quantitative variable) was used to determine the independent relationship between the number and severity of exacerbations and the NLR ratio. The area under the curve (AUC)-ROC was used to determine the predictive capacity of the NLR for severe bronchiectasis, according to the different multidimensional scores. Mean age: 69 (15) years (66.3% of women). The mean NLR was 2.92 (2.03). A higher NLR was associated with more severe bronchiectasis (with an especially significant discriminative power for severe forms) according to the commonly used scores (FACED, E-FACED and BSI), as well as with poorer quality of life (SGRQ), more comorbidities (Charlson index), infection by pathogenic microorganisms, and greater application of treatment. Furthermore, the NLR correlated better with severity scores than other parameters of systemic inflammation. Finally, it was an independent predictor of the incident number and severity of exacerbations. In conclusion, the NLR is an inexpensive and easy-to-measure marker of systemic inflammation for determining severity and predicting exacerbations (especially the most severe) in patients with bronchiectasis.

40. Performance of Serum Angiotensin-Converting Enzyme in Diagnosing Sarcoidosis and Predicting the Active Status of Sarcoidosis: A Meta-Analysis.

Biomolecules

2022 Sep 30

Hu X, Zou L, Wang S

41. Cholinesterase Deficiency Syndrome-A Pitfall in the Use of Butyrylcholinesterase as a Biomarker for Wilson's Disease.

Biomolecules

2022 Sep 30

Arslan M, Novak M, Rosenthal D

42. t(6)A and ms(2)t(6)A Modified Nucleosides in Serum and Urine as Strong Candidate Biomarkers of COVID-19 Infection and Severity.

Biomolecules

2022 Sep 3

Nagayoshi Y, Nishiguchi K, Yamamura R

43. Fibrotic Response of Human Trabecular Meshwork Cells to Transforming Growth Factor-Beta 3 and Autotaxin in Aqueous Humor.

Biomolecules

2022 Sep 3

Liu M, Honjo M, Yamagishi R

44. Role of Adiponectin Peptide I (APNp1) in Age-Related Macular Degeneration.

Biomolecules

2022 Sep 3

Logan C, Lyzogubov V, Bora N

45. Leveraging the Structure of DNAJA1 to Discover Novel Potential Pancreatic Cancer Therapies.

Biomolecules

2022 Sep 29

Roth HE, De Lima Leite A, Palermo NY

46. Molecular Pathogenesis of Cardiac Arrhythmia.

Biomolecules

2022 Sep 29

Okamoto Y, Ono K.

47. Activation of Wnt/β-Catenin Signaling Involves 660 nm Laser Radiation on Epithelium and Modulates Lipid Metabolism.

Biomolecules

2022 Sep 29

Xiao Q, Wang L, Zhang J

48. The Effect of the Classical TSPO Ligand PK 11195 on In Vitro Cobalt Chloride Model of Hypoxia-like Condition in Lung and Brain Cell Lines.

Biomolecules

2022 Sep 29

Amara R, Zeineh N, Monga S

49. Insight on the Role of Leptin: A Bridge from Obesity to Breast Cancer.

Biomolecules

2022 Sep 29

Buonaiuto R, Napolitano F, Parola S

50. Use of Visual Electrophysiology to Monitor Retinal and Optic Nerve Toxicity.

Biomolecules

2022 Sep 29

Chiang TK, White KM, Kurup SK

51. Computational Dissection of the Role of Trp305 in the Regulation of the Death-Associated Protein Kinase-Calmodulin Interaction.

Biomolecules

2022 Sep 29

Zhu YP, Gao XY, Xu GH

52. Characterization of Novel Pectinolytic Enzymes Derived from the Efficient Lignocellulose Degradation Microbiota.

Biomolecules

2022 Sep 29

Miao Q, Zhang X, Wang Y

53. Caenorhabditis elegans as a Model to Study Manganese-Induced Neurotoxicity.

Biomolecules

2022 Sep 29

Martins AC, Gubert P, Li J

Caenorhabditis elegans (C. elegans) is a nematode present worldwide. The worm shows homology to mammalian systems and expresses approximately 40% of human disease-related genes. Since Dr. Sydney Brenner first proposed C. elegans as an advantageous experimental worm-model system for genetic approaches, increasing numbers of studies using C. elegans as a tool to investigate topics in several fields of biochemistry, neuroscience, pharmacology, and toxicology have been performed. In this regard, C. elegans has been used to characterize the molecular mechanisms and affected pathways caused by metals that lead to neurotoxicity, as well as the pathophysiological interrelationship between metal exposure and ongoing neurodegenerative disorders. Several toxic metals, such as lead, cadmium, and mercury, are recognized as important environmental contaminants, and their exposure is associated with toxic effects on the human body. Essential elements that are required to maintain cellular homeostasis and normal physiological functions may also be toxic when accumulated at higher concentrations. For instance, manganese (Mn) is a trace essential element that participates in numerous biological processes, such as enzymatic activities, energy metabolism, and maintenance of cell functions. However, Mn overexposure is associated with behavioral changes in C. elegans, which are consistent with the dopaminergic system being the primary target of Mn neurotoxicity. Caenorhabditis elegans has been shown to be an important tool that allows for studies on neuron morphology using fluorescent transgenic worms. Moreover, behavioral tests may be conducted using worms, and neurotransmitter determination and related gene expression are likely to change after Mn exposure. Likewise, mutant worms may be used to study molecular mechanisms in Mn toxicity, as well as the expression of proteins responsible for the biosynthesis, transport, storage, and uptake of dopamine. Furthermore, this review highlights some advantages and limitations of using the experimental model of C. elegans and provides guidance for potential future applications of this model in studies directed toward assessing for Mn neurotoxicity and related mechanisms.

54. Molecular-Genetic Basis of Plant Breeding.

Biomolecules

2022 Sep 29

Khlestkina E, Shavrukov Y.

55. The Multicellular Effects of VDAC1 N-Terminal-Derived Peptide.

Biomolecules

2022 Sep 28

Anand U, Shteinfer-Kuzmine A, Sela G

56. Calcium-Permeable Channels Cooperation for Rheumatoid Arthritis: Therapeutic Opportunities.

Biomolecules

2022 Sep 27

Liang HY, Yin HX, Li SF

57. Galectin-10 as a Potential Biomarker for Eosinophilic Diseases.

Biomolecules

2022 Sep 27

Tomizawa H, Yamada Y, Arima M

58. Validation of a Commercial Enzyme-Linked Immunosorbent Assay for Allopregnanolone in the Saliva of Healthy Pregnant Women.

Biomolecules

2022 Sep 27

Grötsch MK, Wietor DM, Hettich T

59. Proteins Involved in Plant Dehydration Protection: The Late Embryogenesis Abundant Family.

Biomolecules

2022 Sep 27

Graether SP.

60. Selecting Multitarget Peptides for Alzheimer's Disease.

Biomolecules

2022 Sep 27

Kasus-Jacobi A, Washburn JL, Laurence RB

61. Heterogeneous Clinical Phenotypes of dHMN Caused by Mutation in HSPB1 Gene: A Case Series.

Biomolecules

2022 Sep 27

Shen X, Zhang J, Zhan F

62. Mutational Signatures as Sensors of Environmental Exposures: Analysis of Smoking-Induced Lung Tissue Remodeling.

Biomolecules

2022 Sep 27

Kim YA, Hodzic E, Amgalan B

63. Volumetric Analysis of Glioblastoma for Determining Which CpG Sites Should Be Tested by Pyrosequencing to Predict Temozolomide Efficacy.

Biomolecules

2022 Sep 26

Hosoya T, Takahashi M, Davey C

64. Role of Cell-Cell Junctions in Oesophageal Squamous Cell Carcinoma.

Biomolecules

2022 Sep 26

Xu QR, Du XH, Huang TT

65. Toxic and Essential Metals in Human Health and Disease 2021.

Biomolecules

2022 Sep 26

Aaseth JO.

66. A Pan-Cancer Analysis of Heat-Shock Protein 90 Beta1(HSP90B1) in Human Tumours.

Biomolecules

2022 Sep 26

Wang Y, Wang X.

67. Radiobiological Aspects of FLASH Radiotherapy.

Biomolecules

2022 Sep 26

Hageman E, Che PP, Dahele M

68. Elevated Extracellular HSP72 and Blunted Heat Shock Response in Severe COVID-19 Patients.

Biomolecules

2022 Sep 26

Borges Russo MK, Kowalewski LS, da Natividade GR

AIMS: We hypothesized that critically ill patients with SARS-CoV-2 infection and insulin resistance would present a reduced Heat Shock Response (HSR), which is a pathway involved in proteostasis and anti-inflammation, subsequently leading to worse outcomes and higher inflammation. In this work we aimed: (i) to measure the concentration of extracellular HSP72 (eHSP72) in patients with severe COVID-19 and in comparison with noninfected patients; (ii) to compare the HSR between critically ill patients with COVID-19 (with and without diabetes); and (iii) to compare the HSR in these patients with noninfected individuals. METHODS: Sixty critically ill adults with acute respiratory failure with SARS-CoV-2, with or without diabetes, were selected. Noninfected subjects were included for comparison (healthy, n = 19 and patients with diabetes, n = 22). Blood samples were collected to measure metabolism (glucose and HbA1c); oxidative stress (lypoperoxidation and carbonyls); cytokine profile (IL-10 and TNF); eHSP72; and the HSR (in vitro). RESULTS: Patients with severe COVID-19 presented higher plasma eHSP72 compared with healthy individuals and noninfected patients with diabetes. Despite the high level of plasma cytokines, no differences were found between critically ill patients with COVID-19 with or without diabetes. Critically ill patients, when compared to noninfected, presented a blunted HSR. Oxidative stress markers followed the same pattern. No differences in the HSR (extracellular/intracellular level) were found between critically ill patients, with or without diabetes. CONCLUSIONS: We demonstrated that patients with severe COVID-19 have elevated plasma eHSP72 and that their HSR is blunted, regardless of the presence of diabetes. These results might explain the uncontrolled inflammation and also provide insights on the increased risk in developing type 2 diabetes after SARS-CoV-2 infection.

69. Transcriptome Meta-Analysis Confirms the Hidradenitis Suppurativa Pathogenic Triad: Upregulated Inflammation, Altered Epithelial Organization, and Dysregulated Metabolic Signaling.

Biomolecules

2022 Sep 25

de Oliveira ASLE, Bloise G, Moltrasio C

70. Integration of Nanometer-Range Label-to-Label Distances and Their Distributions into Modelling Approaches.

Biomolecules

2022 Sep 25

Jeschke G.

71. Role of Ion Channels in the Development of Pulmonary Arterial Hypertension.

Biomolecules

2022 Sep 25

Antigny F.

72. Deletion of Growth Hormone Secretagogue Receptor in Kisspeptin Neurons in Female Mice Blocks Diet-Induced Obesity.

Biomolecules

2022 Sep 25

Conde K, Kulyk D, Vanschaik A

73. Matrix Metalloproteinases and Glaucoma.

Biomolecules

2022 Sep 25

Kim MH, Lim SH.

74. Novel Regulatory Roles of Hydrogen Sulfide in Health and Disease.

Biomolecules

2022 Sep 25

Szabo C.

75. Localized Perivascular Therapeutic Approaches to Inhibit Venous Neointimal Hyperplasia in Arteriovenous Fistula Access for Hemodialysis Use.

Biomolecules

2022 Sep 24

Barcena AJR, Perez JVD, Liu O

76. Liposomal Entrapment or Chemical Modification of Relaxin2 for Prolongation of Its Stability and Biological Activity.

Biomolecules

2022 Sep 24

Kogkos G, Gkartziou F, Mourtas S

Relaxin (RLX) is a protein that is structurally similar to insulin and has interesting biological activities. As with all proteins, preservation of RLX's structural integrity/biological functionality is problematic. Herein, we investigated two methods for increasing the duration of relaxin-2's (RLX2) biological activity: synthesis of a palmitoyl RLX2 conjugate (P-RLX2) with the use of a Palmitoyl-l-Glu-OtBu peptide modifier, and encapsulation into liposomes of P-RLX2, RLX2, and its oxidized form (O-RLX2). For liposomal encapsulation thin-film hydration and DRV methods were applied, and different lipid compositions were tested for optimized protein loading. RLX2 and O-RLX2 were quantified by HPLC. The capability of the peptides/conjugate to stimulate transfected cells to produce cyclic adenosine monophosphate (cAMP) was used as a measure of their biological activity. The stability and bioactivity of free and liposomal RLX2 types were monitored for a 30 d period, in buffer (in some cases) and bovine serum (80%) at 37 °C. The results showed that liposome encapsulation substantially increased the RLX2 integrity in buffer; PEGylated liposomes demonstrated a higher protection. Liposome encapsulation also increased the stability of RLX2 and O-RLX2 in serum. Considering the peptide's biological activity, cAMP production of RLX2 was higher than that of the oxidized form and the P-RLX2 conjugate (which demonstrated a similar activity to O-RLX2 when measured in buffer, but lower when measured in the presence of serum proteins), while liposome encapsulation resulted in a slight decrease of bioactivity initially, but prolonged the peptide bioactivity during incubation in serum. It was concluded that liposome encapsulation of RLX2 and synthetic modification to P-RLX2 can both prolong RLX2 peptide in vitro stability; however, the applied chemical conjugation results in a significant loss of bioactivity (cAMP production), whereas the effect of liposome entrapment on RLX2 activity was significantly lower.

77. Myricetin as a Potential Adjuvant in Chemotherapy: Studies on the Inhibition of Human Glutathione Transferase A1-1.

Biomolecules

2022 Sep 24

Alqarni MH, Foudah AI, Muharram MM

78. Cisplatin Resistance: Genetic and Epigenetic Factors Involved.

Biomolecules

2022 Sep 24

Lugones Y, Loren P, Salazar LA.

79. From Contagium vivum fluidum to Riboviria: A Tobacco Mosaic Virus-Centric History of Virus Taxonomy.

Biomolecules

2022 Sep 24

Zerbini FM, Kitajima EW.

80. Prostate Cancer-Associated miRNAs in Saliva: First Steps to an Easily Accessible and Reliable Screening Tool.

Biomolecules

2022 Sep 24

Luedemann C, Reinersmann JL, Klinger C

81. Assessment of Lipid Quality in Commercial Omega-3 Supplements Sold in the French Market.

Biomolecules

2022 Sep 23

Pasini F, Gómez-Caravaca AM, Blasco T

82. Identification of the Thyrotropin-Releasing Hormone (TRH) as a Novel Biomarker in the Prognosis for Acute Myeloid Leukemia.

Biomolecules

2022 Sep 23

Gao Y, Zhou JF, Mao JY

83. Glycosylated Flavonoid Compounds as Potent CYP121 Inhibitors of Mycobacterium tuberculosis.

Biomolecules

2022 Sep 23

Bajrai LH, Khateb AM, Alawi MM

84. Investigating the Role of Zinc in Atherosclerosis: A Review.

Biomolecules

2022 Sep 23

Shen T, Zhao Q, Luo Y

85. A Study on the Nature of SARS-CoV-2 Using the Shell Disorder Models: Reproducibility, Evolution, Spread, and Attenuation.

Biomolecules

2022 Sep 23

Goh GK, Dunker AK, Foster JA

86. The Role of Autophagy in Osteoarthritic Cartilage.

Biomolecules

2022 Sep 23

Kao WC, Chen JC, Liu PC

87. Pulmonary Thrombosis Promotes Tumorigenesis via Myeloid Hypoxia-Inducible Factors.

Biomolecules

2022 Sep 23

Lu X, Prodger A, Sim J

Cancer patients have a greater risk of thrombosis than individuals without cancer. Conversely, thrombosis is a diagnostic predictor of cancer, but the mechanisms by which thrombosis promotes tumor propagation are incompletely understood. Our previous studies showed that hypoxia-inducible factors (HIF) 1α and HIF2α are stabilized in myeloid cells of murine thrombi. We also previously showed that pulmonary thrombosis increases the levels of HIF1α and HIF2α in murine lungs, enhances the levels of tumorigenic factors in the circulation, and promotes pulmonary tumorigenesis. In this study, we aimed to investigate the regulation of thrombosis-induced tumorigenesis by myeloid cell-specific HIFs (i.e., HIF1 and HIF2 in neutrophils and macrophages). Our in vitro studies showed that multiple tumorigenic factors are upregulated in the secretome of hypoxic versus normoxic neutrophils and macrophages, which promotes lung cancer cell proliferation and migration in a myeloid-HIF-dependent manner. Next, we used a mouse model of pulmonary microvascular occlusion to study the impact of pulmonary thrombosis and myeloid HIFs on lung tumorigenesis. Experiments on mice lacking either HIF1α or HIF2α in myeloid cells demonstrated that loss of either factor eliminates the advantage given to pulmonary tumor formation by thrombotic insult. The myeloid HIF-dependent and tumorigenic impact of pulmonary thrombosis on tumor burden may be partly driven by paracrine thymidine phosphorylase (TP), given that TP levels were increased by hypoxia in neutrophil and macrophage supernates, and that plasma TP levels were positively correlated with multiple measures of tumor progression in wild type mice but not myeloid cell-specific HIF1α or HIF2α knockout mice. These data together demonstrate the importance of thrombotic insult in a model of pulmonary tumorigenesis and the essential role of myeloid HIFs in mediating tumorigenic success.

88. Antipsychotic Drug Fluphenazine against Human Cancer Cells.

Biomolecules

2022 Sep 23

Duarte D, Vale N.

89. Functional Gait Assessment Using Manual, Semi-Automated and Deep Learning Approaches Following Standardized Models of Peripheral Nerve Injury in Mice.

Biomolecules

2022 Sep 23

Umansky D, Hagen KM, Chu TH

OBJECTIVE: To develop a standardized model of stretch-crush sciatic nerve injury in mice, and to compare outcomes of crush and novel stretch-crush injuries using standard manual gait and sensory assays, and compare them to both semi-automated as well as deep-learning gait analysis methods. METHODS: Initial studies in C57/Bl6 mice were used to develop crush and stretch-crush injury models followed by histologic analysis. In total, 12 eight-week-old 129S6/SvEvTac mice were used in a six-week behavioural study. Behavioral assessments using the von Frey monofilament test and gait analysis recorded on a DigiGait platform and analyzed through both Visual Gait Lab (VGL) deep learning and standardized sciatic functional index (SFI) measurements were evaluated weekly. At the termination of the study, neurophysiological nerve conduction velocities were recorded, calf muscle weight ratios measured and histological analyses performed. RESULTS: Histological evidence confirmed more severe histomorphological injury in the stretch-crush injured group compared to the crush-only injured group at one week post-injury. Von Frey monofilament paw withdrawal was significant for both groups at week one compared to baseline (p < 0.05), but not between groups with return to baseline at week five. SFI showed hindered gait at week one and two for both groups, compared to baseline (p < 0.0001), with return to baseline at week five. Hind stance width (HSW) showed similar trends as von Frey monofilament test as well as SFI measurements, yet hind paw angle (HPA) peaked at week two. Nerve conduction velocity (NCV), measured six weeks post-injury, at the termination of the study, did not show any significant difference between the two groups; yet, calf muscle weight measurements were significantly different between the two, with the stretch-crush group demonstrating a lower (poorer) weight ratio relative to uninjured contralateral legs (p < 0.05). CONCLUSION: Stretch-crush injury achieved a more reproducible and constant injury compared to crush-only injuries, with at least a Sunderland grade 3 injury (perineurial interruption) in histological samples one week post-injury in the former. However, serial behavioral outcomes were comparable between the two crush groups, with similar kinetics of recovery by von Frey testing, SFI and certain VGL parameters, the latter reported for the first time in rodent peripheral nerve injury. Semi-automated and deep learning-based approaches for gait analysis are promising, but require further validation for evaluation in murine hind-limb nerve injuries.

90. PHLPP Inhibitor NSC74429 Is Neuroprotective in Rodent Models of Cardiac Arrest and Traumatic Brain Injury.

Biomolecules

2022 Sep 23

Jackson TC, Dezfulian C, Vagni VA

91. Tumor Treating Fields (TTFields) Reversibly Permeabilize the Blood-Brain Barrier In Vitro and In Vivo.

Biomolecules

2022 Sep 22

Salvador E, Kessler AF, Domröse D

92. Common Mechanism of Activated Catalysis in P-loop Fold Nucleoside Triphosphatases-United in Diversity.

Biomolecules

2022 Sep 22

Kozlova MI, Shalaeva DN, Dibrova DV

93. GPCRs in Intracellular Compartments: New Targets for Drug Discovery.

Biomolecules

2022 Sep 22

Fasciani I, Carli M, Petragnano F

94. Common Patterns of Hydrolysis Initiation in P-loop Fold Nucleoside Triphosphatases.

Biomolecules

2022 Sep 22

Kozlova MI, Shalaeva DN, Dibrova DV

95. From Small Peptides to Large Proteins against Alzheimer'sDisease.

Biomolecules

2022 Sep 22

Picone P, Sanfilippo T, Vasto S

96. Teenage-Onset Colorectal Cancers in a Digenic Cancer Predisposition Syndrome Provide Clues for the Interaction between Mismatch Repair and Polymerase δ Proofreading Deficiency in Tumorigenesis.

Biomolecules

2022 Sep 22

Schamschula E, Kinzel M, Wernstedt A

97. Biomolecular Mechanisms of Cardiorenal Protection with Sodium-Glucose Co-Transporter 2 Inhibitors.

Biomolecules

2022 Sep 22

Prandi FR, Barone L, Lecis D

98. Human Alpha 1 Antitrypsin Suppresses NF-κB Activity and Extends Lifespan in Adult Drosophila.

Biomolecules

2022 Sep 22

Yuan Y, Van Belkum M, O'Brien A

99. Potassium Channels as Therapeutic Targets in Pulmonary Arterial Hypertension.

Biomolecules

2022 Sep 22

Redel-Traub G, Sampson KJ, Kass RS

100. The Acute and Short-Term Inhalation of Carbon Nanofiber in Sprague-Dawley Rats.

Biomolecules

2022 Sep 22

Jo MS, Kim BW, Kim YH

101. Emerging Role of Non-Coding RNAs in Aortic Dissection.

Biomolecules

2022 Sep 21

Ding W, Liu Y, Su Z

102. The Stability and Anti-Angiogenic Properties of Titanium Dioxide Nanoparticles (TiO(2)NPs) Using Caco-2 Cells.

Biomolecules

2022 Sep 21

David OM, Lategan KL, de Cortalezzi MF

103. A Programmable, DNA-Exclusively-Guided Argonaute DNase and Its Higher Cleavage Specificity Achieved by 5'-Hydroxylated Guide.

Biomolecules

2022 Sep 21

Sun S, Xu D, Zhu L

104. Implication of Nanoparticles to Combat Chronic Liver and Kidney Diseases: Progress and Perspectives.

Biomolecules

2022 Sep 21

Hashim M, Mujahid H, Hassan S

105. Obtaining a Reliable Diagnostic Biomarker for Diabetes Mellitus by Standardizing Salivary Glucose Measurements.

Biomolecules

2022 Sep 21

Cui Y, Zhang H, Wang S

106. Ractopamine at the Center of Decades-Long Scientific and Legal Disputes: A Lesson on Benefits, Safety Issues, and Conflicts.

Biomolecules

2022 Sep 21

Abbas K, Raza A, Vasquez RD

107. Liraglutide Attenuates Glucolipotoxicity-Induced RSC96 Schwann Cells' Inflammation and Dysfunction.

Biomolecules

2022 Sep 21

Kornelius E, Tsou SH, Chang CC

108. Association of Maternal Diet during Pregnancy and Metabolite Profile in Cord Blood.

Biomolecules

2022 Sep 21

Harris CP, Ramlochansingh C, Uhl O

109. Curcumin Targeting Non-Coding RNAs in Colorectal Cancer: Therapeutic and Biomarker Implications.

Biomolecules

2022 Sep 21

Li J, Chai R, Chen Y

110. Fingerprinting" Benign and Cancerous Skin Lesions Using Vibrational Optical Coherence Tomography: Differentiation among Cancerous Lesion Types Based on the Presence of New Cells, Blood Vessels, and Fibrosis.

Biomolecules

2022 Sep 21

Silver FH, Deshmukh T, Ryan N

In this study, we use vibrational optical coherence tomography (VOCT) to examine the morphology and stiffness of benign and cancerous lesions. Lesion images and 3D plots of weighted displacement versus frequency and depth were used to compare the cellular, dermal collagen, new blood vessels, and fibrotic composition of normal skin, actinic keratoses (AK), nodular and superficial basal cell carcinomas (BCCs), squamous cell carcinomas (SCCs), and melanomas. The results of this study suggest that benign and cancerous lesions differ based on the addition of new cells with increased resonant frequency and stiffness (80 Hz, 1.8 MPa), new blood vessel peaks (130 Hz, 4.10 MPa) that appear to be less stiff than normal blood vessels, and new fibrous tissue peaks (260 Hz, 15-17 MPa) that are present in carcinomas but not in normal skin and only partially present (80 Hz and 130 Hz only) in AKs. Results obtained by creating images based on the location of the 80 Hz, 130 Hz, and 260 Hz peaks of cancerous skin lesions suggest that the fibrous tissue appears to surround the new cells and new lesion blood vessels. The results of this study suggest that the morphology and location of the fibrous tissues in relation to the new cancer-associated cells and lesion blood vessels may provide information on the invasiveness and metastatic potential of skin cancers. The invasiveness and metastatic potential of melanomas may be a result of the cancer-associated cells laying down fibrous tissue that is used as a pathway for migration. The new cancer-associated blood vessels in the vicinity of the new cancer-associated cells may promote this migration and eventual metastasis. The ratios of peak heights 50/130 Hz and 80/130 Hz of normal cells, new lesion cells, new lesion blood vessels, and fibrotic tissue may be used as a "fingerprint" for detecting melanoma and to differentiate it from other skin cancers non-invasively using VOCT.

111. Shortened Hinge Design of Fab x sdAb-Fc Bispecific Antibodies Enhances Redirected T-Cell Killing of Tumor Cells.

Biomolecules

2022 Sep 21

Huang S, Segués A, Waterfall M

112. Fibrillin-1 Regulates Arteriole Integrity in the Retina.

Biomolecules

2022 Sep 20

Alonso F, Li L, Fremaux I

113. Anti-Phosphatidylethanolamine and Anti-Phosphatidylserine Antibodies-Association with Renal Involvement, Atherosclerosis, Cardiovascular Manifestations, Raynaud Phenomenon and Disease Activity in Polish Patients with Systemic Lupus Erythematosus.

Biomolecules

2022 Sep 20

Fischer K, Przepiera-Będzak H, Brzosko I

OBJECTIVE: To evaluate the association between anti-phosphatidylethanolamine (aPE) and anti-phosphatidylserine (aPS) antibodies and cardiovascular risk, organ involvement and disease activity in systemic lupus erythematosus (SLE) patients. METHODS: We studied 93 SLE patients and 30 controls. We analyzed levels of anti-phospholipid antibodies, including aPS and aPE, the profiles of antinuclear, anti-neutrophil cytoplasmic (ANCA) and anti-endothelial antibodies, carotid intima-media thickness (cITM) and atherosclerotic plaque presence, ankle-brachial and high resistance indices, atherosclerotic risk factors, organ manifestations and treatment. RESULTS: Levels of aPS and aPE were significantly higher in SLE patients in comparison with the controls (p = 0.038 and p = 0.044, respectively). aPS was associated with the risk of Raynaud's phenomenon (p = 0.021) development. aPE increased the risk of renal involvement (p = 0.049), cerebral stroke (p = 0.050), high vlues of cIMT (p = 0.041) development as well as occurrence of selected serological markers associated with activity of the disease such as anti-double stranded DNA (p = 0.021). The long duration of regular smoking (p = 0.021) and the high number of cigarettes/day (p = 0.015) were significantly associated with the risk of aPE occurrence. CONCLUSIONS: Patients with aPS and aPE are at risk of vascular involvement. Especially the presence of aPE may significantly increase the risk of thrombotic complications development in SLE patients without classical serological markers of APS. Finally, aPE might be used as a marker of disease activity and risk of renal injury development in this patient group. The classical atherosclerotic markers including lipid indices play an important role in complex analysis of cardiovascular risk in lupus patients and enable to identify patients at the highest risk and implement effective preventive, diagnostic and therapeutic procedures.

114. Why the Ala-His-His Peptide Is an Appropriate Scaffold to Remove and Redox Silence Copper Ions from the Alzheimer's-Related Aβ Peptide.

Biomolecules

2022 Sep 20

Gonzalez P, Sabater L, Mathieu E

115. Radical Mediated Rapid In Vitro Formation of c-Type Cytochrome.

Biomolecules

2022 Sep 20

Ibrahim SM, Ben Aoun S, Nakajima H

116. Bioactive Molecules: Structures, Functions, and Potential Uses for Cancer Prevention and Targeted Therapies.

Biomolecules

2022 Sep 2

Dou Q.

117. Identification of a Multi-Messenger RNA Signature as Type 2 Diabetes Mellitus Candidate Genes Involved in Crosstalk between Inflammation and Insulin Resistance.

Biomolecules

2022 Sep 2

Ali HS, Boshra MS, Agwa SHA

118. Role of Reactive Oxygen Species in Glucose Metabolism Disorder in Diabetic Pancreatic β-Cells.

Biomolecules

2022 Sep 2

Mukai E, Fujimoto S, Inagaki N.

119. Oxidative Stress and NRF2/KEAP1/ARE Pathway in Diabetic Kidney Disease (DKD): New Perspectives.

Biomolecules

2022 Sep 2

Tanase DM, Gosav EM, Anton MI

120. PD-L1-Mediated Immunosuppression in Hepatocellular Carcinoma: Relationship with Macrophages Infiltration and Inflammatory Response Activity.

Biomolecules

2022 Sep 2

Guo S, Wang X, Zhou H

121. The Nicotinamide/Streptozotocin Rodent Model of Type 2 Diabetes: Renal Pathophysiology and Redox Imbalance Features.

Biomolecules

2022 Sep 2

Yan LJ.

122. Role of WTAP in Cancer: From Mechanisms to the Therapeutic Potential.

Biomolecules

2022 Sep 2

Fan Y, Li X, Sun H

123. Transcriptome Analyses Identify Deregulated MYC in Early Onset Colorectal Cancer.

Biomolecules

2022 Sep 2

Marx OM, Mankarious MM, Eshelman MA

124. The Metabolomic Approach for the Screening of Endometrial Cancer: Validation from a Large Cohort of Women Scheduled for Gynecological Surgery.

Biomolecules

2022 Sep 2

Troisi J, Mollo A, Lombardi M

125. Regenerative Potential of A Bovine ECM-Derived Hydrogel for Biomedical Applications.

Biomolecules

2022 Sep 2

Di Francesco D, Bertani F, Fusaro L

126. Generation and Validation of an Anti-Human PANK3 Mouse Monoclonal Antibody.

Biomolecules

2022 Sep 19

Khadka S, Vien L, Leonard P

127. Exploring Biomolecular Self-Assembly with Far-Infrared Radiation.

Biomolecules

2022 Sep 19

Kawasaki T, Yamaguchi Y, Kitahara H

128. RetroComposer: Composing Templates for Template-Based Retrosynthesis Prediction.

Biomolecules

2022 Sep 19

Yan C, Zhao P, Lu C

129. Myocarditis-like Episodes in Patients with Arrhythmogenic Cardiomyopathy: A Systematic Review on the So-Called Hot-Phase of the Disease.

Biomolecules

2022 Sep 19

Bariani R, Rigato I, Cipriani A

Arrhythmogenic cardiomyopathy (ACM) is a genetically determined myocardial disease, characterized by myocytes necrosis with fibrofatty substitution and ventricular arrhythmias that can even lead to sudden cardiac death. The presence of inflammatory cell infiltrates in endomyocardial biopsies or in autoptic specimens of ACM patients has been reported, suggesting a possible role of inflammation in the pathophysiology of the disease. Furthermore, chest pain episodes accompanied by electrocardiographic changes and troponin release have been observed and defined as the "hot-phase" phenomenon. The aim of this critical systematic review was to assess the clinical features of ACM patients presenting with "hot-phase" episodes. According to PRISMA guidelines, a search was run in the PubMed, Scopus and Web of Science electronic databases using the following keywords: "arrhythmogenic cardiomyopathy"; "myocarditis" or "arrhythmogenic cardiomyopathy"; "troponin" or "arrhythmogenic cardiomyopathy"; and "hot-phase". A total of 1433 titles were retrieved, of which 65 studies were potentially relevant to the topic. Through the application of inclusion and exclusion criteria, 9 papers reporting 103 ACM patients who had experienced hot-phase episodes were selected for this review. Age at time of episodes was available in 76% of cases, with the mean age reported being 26 years ± 14 years (min 2-max 71 years). Overall, 86% of patients showed left ventricular epicardial LGE. At the time of hot-phase episodes, 49% received a diagnosis of ACM (Arrhythmogenic left ventricular cardiomyopathy in the majority of cases), 19% of dilated cardiomyopathy and 26% of acute myocarditis. At the genetic study, Desmoplakin (DSP) was the more represented disease-gene (69%), followed by Plakophillin-2 (9%) and Desmoglein-2 (6%). In conclusion, ACM patients showing hot-phase episodes are usually young, and DSP is the most common disease gene, accounting for 69% of cases. Currently, the role of "hot-phase" episodes in disease progression and arrhythmic risk stratification remains to be clarified.

130. The Paradoxical Role of Circulating Ketone Bodies in Glycemic Control of Individuals with Type 2 Diabetes: High Risk, High Reward?

Biomolecules

2022 Sep 18

van der Vaart A, Knol MGE, de Borst MH

INTRODUCTION: Fasting plasma ketone bodies (KB) are elevated in individuals with type 2 diabetes (T2D) and could affect glycemic control and disease progression. Prolonged KB exposure may result in adaptive beneficial responses, counteracting glycemic dysregulation. In the current proof-of-concept study in adults with T2D, we hypothesized that fasting plasma KB are cross-sectionally associated with poorer glycemic control but prospectively with better glycemic control. MATERIALS AND METHODS: Fasting plasma KB were measured via nuclear magnetic resonance spectroscopy in patients with T2D treated in primary care (Zodiac cohort; The Netherlands). We analyzed the associations between KB and HbA1c at baseline using linear regression analyses and HbA1c changes over time using linear mixed models. We adjusted for potential confounders, including risk factors for poor glycemic control. Individuals with T2D participating in the general population-based PREVEND study were used as a replication cohort. RESULTS: We included 271 individuals with T2D with a total of 859 HbA1c measurements during a follow-up period of 3.0 (2.0-3.2) years. At baseline, the total amount of fasting plasma KB was independently and positively associated with HbA1c levels (regression coefficient in the fully adjusted analysis = 0.31; 95% CI 0.06-0.57, per doubling of KB; p = 0.02). In contrast, in the longitudinal analyses, fasting plasma KB were associated with a yearly HbA1c (%) decrease of -0.10 (95% CI -0.19 to -0.00 per doubling baseline KB; p = 0.05). Results were replicated in 387 individuals with T2D from a general population cohort with a total of 1115 glucose measurements during a follow-up period of 7.5 (7.2-8.0) years. A yearly decrease in fasting plasma glucose (mmol/L) of 0.09 was found per doubling of baseline KB. CONCLUSIONS: This study is the first to suggest a paradoxical role of circulating KB on glycemic control in T2D: elevated KB are associated with cross-sectionally poorer glycemic control but longitudinally with better long-term glycemic control.

131. Roles of Two-Component Signal Transduction Systems in Shigella Virulence.

Biomolecules

2022 Sep 18

Pasqua M, Coluccia M, Eguchi Y

132. Controversial Role of Transferrin in the Transport of Ruthenium Anticancer Drugs.

Biomolecules

2022 Sep 18

Levina A, Chetcuti ARM, Lay PA.

133. Biphasic Response of Astrocytic Brain-Derived Neurotrophic Factor Expression following Corticosterone Stimulation.

Biomolecules

2022 Sep 18

Tsimpolis A, Kokkali M, Logothetis A

134. Role of circRNA in E3 Modification under Human Disease.

Biomolecules

2022 Sep 18

Chen Z, Song M, Wang T

135. Injectable Peptide Hydrogel Encapsulation of Mesenchymal Stem Cells Improved Viability, Stemness, Anti-Inflammatory Effects, and Early Stage Wound Healing.

Biomolecules

2022 Sep 17

Li Q, Qi G, Lutter D

136. Expression of Major Lipid Raft Protein Raftlin in Chronic Rhinosinusitis with Nasal Polyps in Smoking and Non-Smoking Patients Correlated with Interleukin-17 and Tumor Necrosis Factor-α Levels.

Biomolecules

2022 Sep 17

Lin YT, Tsai MH, Su YY

137. Digital Pathology: New Initiative in Pathology.

Biomolecules

2022 Sep 17

Cho WC.

138. Plasma Kallikrein-Activated TGF-β Is Prognostic for Poor Overall Survival in Patients with Pancreatic Ductal Adenocarcinoma and Associates with Increased Fibrogenesis.

Biomolecules

2022 Sep 17

Pedersen RS, Nissen NI, Jensen C

139. Glycosylation-The Most Diverse Post-Translational Modification.

Biomolecules

2022 Sep 17

Staudacher E, Van Damme EJM, Smagghe G.

140. Ginsenoside and Its Therapeutic Potential for Cognitive Impairment.

Biomolecules

2022 Sep 16

Feng H, Xue M, Deng H

141. Effects of RIPC on the Metabolome in Patients Undergoing Vascular Surgery: A Randomized Controlled Trial.

Biomolecules

2022 Sep 16

Eerik K, Kasepalu T, Kuusik K

142. Phenolics from Defatted Black Cumin Seeds (Nigella sativa L.): Ultrasound-Assisted Extraction Optimization, Comparison, and Antioxidant Activity.

Biomolecules

2022 Sep 16

Gueffai A, Gonzalez-Serrano DJ, Christodoulou MC

143. Aerosol-Administered Adelmidrol Attenuates Lung Inflammation in a Murine Model of Acute Lung Injury.

Biomolecules

2022 Sep 16

Interdonato L, D'amico R, Cordaro M

144. Phytochemicals in Inhibition of Prostate Cancer: Evidence from Molecular Mechanisms Studies.

Biomolecules

2022 Sep 16

Hao Q, Wu Y, Vadgama JV

145. Phytohormones 2020.

Biomolecules

2022 Sep 16

Kudoyarova G.

146. Chitosan as an Adjuvant to Enhance the Control Efficacy of Low-Dosage Pyraclostrobin against Powdery Mildew of Rosa roxburghii and Improve Its Photosynthesis, Yield, and Quality.

Biomolecules

2022 Sep 16

Zhang C, Li Q, Li J

147. 6-Furopyridine Hexamethylene Amiloride Is a Non-Selective P2X7 Receptor Antagonist.

Biomolecules

2022 Sep 16

Cuthbertson P, Elhage A, Al-Rifai D

148. Functional and Structural Impact of Deleterious Missense Single Nucleotide Polymorphisms in the NR3C1, CYP3A5, and TNF-α Genes: An In Silico Analysis.

Biomolecules

2022 Sep 16

Ramayanam NR, Manickam R, Mahalingam VT

149. The Association of Metformin, Other Antidiabetic Medications and Statins on the Prognosis of Rectal Cancer in Patients with Type 2 Diabetes: A Retrospective Cohort Study.

Biomolecules

2022 Sep 15

Erkinantti S, Hautakoski A, Sund R

150. The Role of Disordered Regions in Orchestrating the Properties of Multidomain Proteins: The SARS-CoV-2 Nucleocapsid Protein and Its Interaction with Enoxaparin.

Biomolecules

2022 Sep 15

Schiavina M, Pontoriero L, Tagliaferro G

151. Oviductal Extracellular Vesicles Enhance Porcine In Vitro Embryo Development by Modulating the Embryonic Transcriptome.

Biomolecules

2022 Sep 15

de Alcântara-Neto AS, Cuello C, Uzbekov R

152. Co-Stimulatory Receptor Signaling in CAR-T Cells.

Biomolecules

2022 Sep 15

Honikel MM, Olejniczak SH.

153. Conservation Biology and Reproduction in a Time of Developmental Plasticity.

Biomolecules

2022 Sep 14

Holt WV, Comizzoli P.

154. Bim Expression Modulates Branching Morphogenesis of the Epithelium and Endothelium.

Biomolecules

2022 Sep 14

Sorenson CM, Song YS, Wang S

155. Progesterone as an Anti-Inflammatory Drug and Immunomodulator: New Aspects in Hormonal Regulation of the Inflammation.

Biomolecules

2022 Sep 14

Fedotcheva TA, Fedotcheva NI, Shimanovsky NL.

The specific regulation of inflammatory processes by steroid hormones has been actively studied in recent years, especially by progesterone (P4) and progestins. The mechanisms of the anti-inflammatory and immunomodulatory P4 action are not fully clear. The anti-inflammatory effects of P4 can be defined as nonspecific, associated with the inhibition of NF-κB and COX, as well as the inhibition of prostaglandin synthesis, or as specific, associated with the regulation of T-cell activation, the regulation of the production of pro- and anti-inflammatory cytokines, and the phenomenon of immune tolerance. The specific anti-inflammatory effects of P4 and its derivatives (progestins) can also include the inhibition of proliferative signaling pathways and the antagonistic action against estrogen receptor beta-mediated signaling as a proinflammatory and mitogenic factor. The anti-inflammatory action of P4 is accomplished through the participation of progesterone receptor (PR) chaperones HSP90, as well as immunophilins FKBP51 and FKBP52, which are the validated targets of clinically approved immunosuppressive drugs. The immunomodulatory and anti-inflammatory effects of HSP90 inhibitors, tacrolimus and cyclosporine, are manifested, among other factors, due to their participation in the formation of an active ligand-receptor complex of P4 and their interaction with its constituent immunophilins. Pharmacological agents such as HSP90 inhibitors can restore the lost anti-inflammatory effect of glucocorticoids and P4 in chronic inflammatory and autoimmune diseases. By regulating the activity of FKBP51 and FKBP52, it is possible to increase or decrease hormonal signaling, as well as restore it during the development of hormone resistance. The combined action of immunophilin suppressors with steroid hormones may be a promising strategy in the treatment of chronic inflammatory and autoimmune diseases, including endometriosis, stress-related disorders, rheumatoid arthritis, and miscarriages. Presumably, the hormone receptor- and immunophilin-targeted drugs may act synergistically, allowing for a lower dose of each.

156. Generation and Functional Characterization of PLAP CAR-T Cells against Cervical Cancer Cells.

Biomolecules

2022 Sep 14

Yekehfallah V, Pahlavanneshan S, Sayadmanesh A

157. Clinical and Mechanistic Review of Amiodarone-Associated Optic Neuropathy.

Biomolecules

2022 Sep 14

Mitchell R, Chacko J.

158. Parental Programming of Offspring Health: The Intricate Interplay between Diet, Environment, Reproduction and Development.

Biomolecules

2022 Sep 13

Batra V, Norman E, Morgan HL

159. Glutathione-S-Transferases as Potential Targets for Modulation of Nitric Oxide-Mediated Vasodilation.

Biomolecules

2022 Sep 13

Russell TM, Richardson DR.

160. GS-2: A Novel Broad-Spectrum Agent for Environmental Microbial Control.

Biomolecules

2022 Sep 13

Mayfosh AJ, Day ZI, Unsworth NB

161. Soluble Protein Hydrolysate Ameliorates Gastrointestinal Inflammation and Injury in 2,4,6-Trinitrobenzene Sulfonic Acid-Induced Colitis in Mice.

Biomolecules

2022 Sep 13

Wei J, Tao G, Xu B

Inflammatory bowel diseases (IBD) are chronic, recurring gastrointestinal diseases that severely impair health and quality of life. Although therapeutic options have significantly expanded in recent years, there is no effective therapy for a complete and permanent cure for IBD. Well tolerated dietary interventions to improve gastrointestinal health in IBD would be a welcome advance especially with anticipated favorable tolerability and affordability. Soluble protein hydrolysate (SPH) is produced by the enzymatic hydrolysis of commercial food industry salmon offcuts (consisting of the head, backbone and skin) and contains a multitude of bioactive peptides including those with anti-oxidant properties. This study aimed to investigate whether SPH ameliorates gastrointestinal injury in 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced mouse colitis model. Mice were randomly assigned to four groups: Control (no colitis), Colitis, Colitis/CP (with control peptide treatment), and Colitis/SPH (with SPH treatment). Colitis was induced by cutaneous sensitization with 1% TNBS on day -8 followed by 2.5% TNBS enema challenge on day 0. Control peptides and SPH were provided to the mice in the Colitis/CP or Colitis/SPH group respectively by drinking water at the final concentration of 2% w/v daily from day -10 to day 4. Then, the colon was harvested on day 4 and examined macro- and microscopically. Relevant measures included disease activity index (DAI), colon histology injury, immune cells infiltration, pro- and anti-inflammatory cytokines and anti-oxidative gene expression. It was found that SPH treatment decreased the DAI score and colon tissue injury when compared to the colitis-only and CP groups. The protective mechanisms of SPH were associated with reduced infiltration of CD4+ T, CD8+ T and B220+ B lymphocytes but not macrophages, downregulated pro-inflammatory cytokines (tumor necrosis factor-α and interleukin-6), and upregulated anti-inflammatory cytokines (transforming growth factor-β1 and interleukin-10) in the colon tissue. Moreover, the upregulation of anti-oxidative genes, including ferritin heavy chain 1, heme oxygenase 1, NAD(P)H quinone oxidoreductase 1, and superoxide dismutase 1, in the colons of colitis/SPH group was observed compared with the control peptide treatment group. In conclusion, the protective mechanism of SPH is associated with anti-inflammatory and anti-oxidative effects as demonstrated herein in an established mice model of colitis. Clinical studies with SPH as a potential functional food for the prevention or as an adjuvant therapy in IBD may add an effective and targeted diet-based approach to IBD management in the future.

162. In Silico Maturation of a Nanomolar Antibody against the Human CXCR2.

Biomolecules

2022 Sep 13

Buratto D, Wan Y, Shi X

163. Comparative Transcriptomic Analysis of mRNAs, miRNAs and lncRNAs in the Longissimus dorsi Muscles between Fat-Type and Lean-Type Pigs.

Biomolecules

2022 Sep 13

Zhang J, Wang J, Ma C

In pigs, meat quality and production are two important traits affecting the pig industry and human health. Compared to lean-type pigs, fat-type pigs contain higher intramuscular fat (IMF) contents, better taste and nutritional value. To uncover genetic factors controlling differences related to IMF in pig muscle, we performed RNA-seq analysis on the transcriptomes of the Longissimus dorsi (LD) muscle of Laiwu pigs (LW, fat-type pigs) and commercial Duroc × Landrace × Yorkshire pigs (DLY, lean-type pigs) at 150 d to compare the expression profiles of mRNA, miRNA and lncRNA. A total of 225 mRNAs, 12 miRNAs and 57 lncRNAs were found to be differentially expressed at the criteria of |log2(foldchange)| > 1 and q < 0.05. The mRNA expression of LDHB was significantly higher in the LD muscle of LW compared to DLY pigs with log2(foldchange) being 9.66. Using protein interaction prediction method, we identified more interactions of estrogen-related receptor alpha (ESRRA) associated with upregulated mRNAs, whereas versican (VCAN) and proenkephalin (PENK) were associated with downregulated mRNAs in LW pigs. Integrated analysis on differentially expressed (DE) mRNAs and miRNAs in the LD muscle between LW and DLY pigs revealed two network modules: between five upregulated mRNA genes (GALNT15, FKBP5, PPARGC1A, LOC110258214 and LOC110258215) and six downregulated miRNA genes (ssc-let-7a, ssc-miR190-3p, ssc-miR356-5p, ssc-miR573-5p, ssc-miR204-5p and ssc-miR-10383), and between three downregulated DE mRNA genes (IFRD1, LOC110258600 and LOC102158401) and six upregulated DE miRNA genes (ssc-miR1379-3p, ssc-miR1379-5p, ssc-miR397-5p, ssc-miR1358-5p, ssc-miR299-5p and ssc-miR1156-5p) in LW pigs. Based on the mRNA and ncRNA binding site targeting database, we constructed a regulatory network with miRNA as the center and mRNA and lncRNA as the target genes, including GALNT15/ssc-let-7a/LOC100523888, IFRD1/ssc-miR1379-5p/CD99, etc., forming a ceRNA network in the LD muscles that are differentially expressed between LW and DLY pigs. Collectively, these data may provide resources for further investigation of molecular mechanisms underlying differences in meat traits between lean- and fat-type pigs.

164. The Protective Effect of Simvastatin on the Systolic Function of the Heart in the Model of Acute Ischemia and Reperfusion Is Due to Inhibition of the RhoA Pathway and Independent of Reduction of MMP-2 Activity.

Biomolecules

2022 Sep 13

Skrzypiec-Spring M, Sapa-Wojciechowska A, Rak-Pasikowska A

165. Building Protein Atomic Models from Cryo-EM Density Maps and Residue Co-Evolution.

Biomolecules

2022 Sep 13

Bouvier G, Bardiaux B, Pellarin R

166. NMR-Based Metabolomic Analysis for the Effects of Trimethylamine N-Oxide Treatment on C2C12 Myoblasts under Oxidative Stress.

Biomolecules

2022 Sep 13

Zou H, Huang C, Zhou L

167. Galectokines: The Promiscuous Relationship between Galectins and Cytokines.

Biomolecules

2022 Sep 13

Sanjurjo L, Broekhuizen EC, Koenen RR

168. Enzymatic Potential of Filamentous Fungi as a Biological Pretreatment for Acidogenic Fermentation of Coffee Waste.

Biomolecules

2022 Sep 12

Pereira J, Cachinho A, de Melo MMR

169. DNA Methylation in Regulatory T Cell Differentiation and Function: Challenges and Opportunities.

Biomolecules

2022 Sep 12

Bai L, Hao X, Keith J

170. Effects of Nickel at Environmentally Relevant Concentrations on Human Corneal Epithelial Cells: Oxidative Damage and Cellular Apoptosis.

Biomolecules

2022 Sep 12

Zhang ZN, Liu H, Liu MM

171. Plasma Clearance of Intravenously Infused Adrenomedullin in Rats with Acute Renal Failure.

Biomolecules

2022 Sep 11

Hosoda H, Nakamura T, Yoshihara F.

172. Preparation, Characterization and Multiple Biological Properties of Peptide-Modified Cerium Oxide Nanoparticles.

Biomolecules

2022 Sep 10

Wang M, He H, Liu D

173. New Insights into the Alveolar Epithelium as a Driver of Acute Respiratory Distress Syndrome.

Biomolecules

2022 Sep 10

Sanches Santos Rizzo Zuttion M, Moore SKL, Chen P

174. Identification of Novel Ribonucleotide Reductase Inhibitors for Therapeutic Application in Bile Tract Cancer: An Advanced Pharmacoinformatics Study.

Biomolecules

2022 Sep 10

Islam MA, Barshetty MM, Srinivasan S

175. Identification of Unique Genetic Biomarkers of Various Subtypes of Glomerulonephritis Using Machine Learning and Deep Learning.

Biomolecules

2022 Sep 10

Qing J, Zheng F, Zhi H

176. The Regulators of Human Endometrial Stromal Cell Decidualization.

Biomolecules

2022 Sep 10

Murata H, Tanaka S, Okada H.

177. Araufuranone: A New Phytotoxic Tetrasubstituted Dihydrofuro[3,2-b]furan-2(5H)-One Isolated from Ascochyta araujiae.

Biomolecules

2022 Sep 10

Masi M, Boari A, Sautua F

178. Integrating Text Mining into the Curation of Disease Maps.

Biomolecules

2022 Sep 10

Voskamp M, Vinhoven L, Stanke F

179. Urinary Angiotensinogen and Progression of Chronic Kidney Disease: Results from KNOW-CKD Study.

Biomolecules

2022 Sep 10

Suh SH, Oh TR, Choi HS

180. Metabolic Abnormalities Linked to Auditory Pathways in ApoE-Knockout HEI-OC1 Cells: A Transcription-Metabolism Co-Analysis.

Biomolecules

2022 Sep 1

Ma L, Wang H, Yao J

181. Inhibition of CYP2C8 by Acyl Glucuronides of Gemfibrozil and Clopidogrel: Pharmacological Significance, Progress and Challenges.

Biomolecules

2022 Sep 1

Shah MB.

182. Mitochondrial Dysfunction in Rheumatoid Arthritis.

Biomolecules

2022 Sep 1

Ma C, Wang J, Hong F

183. Changes in NMDA Receptor Function in Rapid Ischemic Tolerance: A Potential Role for Tri-Heteromeric NMDA Receptors.

Biomolecules

2022 Sep 1

Xie M, Leng T, Maysami S

184. Plasma of Argon Treatment of the Implant Surface, Systematic Review of In Vitro Studies.

Biomolecules

2022 Sep 1

Carossa M, Cavagnetto D, Mancini F

185. Polyphenolic Compounds Inhibit Osteoclast Differentiation While Reducing Autophagy through Limiting ROS and the Mitochondrial Membrane Potential.

Biomolecules

2022 Sep 1

Laha D, Sarkar J, Maity J

Polyphenolic compounds are a diverse group of natural compounds that interact with various cellular proteins responsible for cell survival, differentiation, and apoptosis. However, it is yet to be established how these compounds interact in myeloid cells during their differentiation and the molecular and intracellular mechanisms involved. Osteoclasts are multinucleated cells that originate from myeloid cells. They resorb cartilage and bone, maintain bone homeostasis, and can cause pathogenesis. Autophagy is a cellular mechanism that is responsible for the degradation of damaged proteins and organelles within cells and helps maintain intracellular homeostasis. Imbalances in autophagy cause various pathological disorders. The current study investigated the role of several polyphenolic compounds, including tannic acid (TA), gallic acid (GA), and ellagic acid (EA) in the regulation of osteoclast differentiation of myeloid cells. We demonstrated that polyphenolic compounds inhibit osteoclast differentiation in a dose-dependent manner. Quantitative real-time PCR, immunocytochemistry, and western blotting revealed that osteoclast markers, such as NFATc1, Cathepsin K, and TRAP were inhibited after the addition of polyphenolic compounds during osteoclast differentiation. In our investigation into the molecular mechanisms, we found that the addition of polyphenolic compounds reduced the number of autophagic vesicles and the levels of LC3B, BECN1, ATG5, and ATG7 molecules through the inactivation of Akt, thus inhibiting the autophagy process. In addition, we found that by decreasing intracellular calcium and decreasing ROS levels, along with decreasing mitochondrial membrane potential, polyphenolic compounds inhibit osteoclast differentiation. Together, this study provides evidence that polyphenolic compounds inhibit osteoclast differentiation by reducing ROS production, autophagy, intracellular Ca2+ level, and mitochondrial membrane potentials.

186. Differential Protein Content between Fresh and Freeze-Dried Plasma Rich in Growth Factors Eye Drops.

Biomolecules

2022 Sep 1

Anitua E, Pino A, Azkargorta M

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