BRASSINOSTEROID INSENSITIVE1-ASSOCIATED KINASE1 (BAK1) is a co-receptor involved in the recognition of pattern-associated molecular patterns (PAMPs) via plasma membrane-localized pattern recognition receptors (PRRs). Absence of BAK1/SERK4 leads to the activation of autoimmunity in plants. Yu et al. recently showed that BAK-TO-LIFE 2 (BTL2) is required for the surveillance of BAK1/SERK4 integrity to maintain immune homeostasis.

To support a global population of ~10 billion people in 2050, dietary protein demand is forecast to increase 32-78% compared to 2017, requiring significantly higher planetary resources. Microalgae are an attractive sustainable protein source compared with current plant and animal sources. Benefits include mass scalability, low CO2 emissions, and significantly reduced land and freshwater use per unit protein. Microalgae are already used as food products and numerous species exhibit high total protein contents and well-balanced essential amino acid (EAA) compositions for human dietary requirements. Microalgae proteins are also bioavailable for human digestion, and downstream processing steps are likely to further enhance protein digestibility. Species, cultivation, and process/product optimisation are actively being developed to enhance their nutritional, social, and environmental benefits.

Hulled grains, while providing natural protection for seeds, pose a challenge to manual threshing due to the pair of glumes tightly encasing them. Based on natural evolution and artificial domestication, gramineous crops evolved various hull-like floral organs. Recently, progress has been made in uncovering novel domesticated genes associated with cereal threshability and deciphering common regulatory modules pertinent to the specification of hull-like floral organs. Here we review morphological similarities, principal regulators, and common mechanisms implicated in the easy-threshing traits of crops. Understanding the shared and unique features in the developmental process of cereal threshability may not only shed light on the convergent evolution of cereals but also facilitate the de novo domestication of wild cereal germplasm resources through genome-editing technologies.

New organelle acquisition through neofunctionalization of the endomembrane system (ES) with respect to plant secondary metabolism is a key evolutionary strategy for plant adaptation, which is overlooked due to the complexity of angiosperms. Bryophytes produce a broad range of plant secondary metabolites (PSMs), and their simple cellular structures, including unique organelles, such as oil bodies (OBs), highlight them as suitable model to investigate the contribution of the ES to PSMs. In this opinion, we review latest findings on the contribution of the ES to PSM biosynthesis, with a specific focus on OBs, and propose that the ES provides organelles and trafficking routes for PSM biosynthesis, transportation, and storage. Therefore, future research on ES-derived organelles and trafficking routes will provide essential knowledge for synthetic applications.

In the future, 8-10 billion people will need to be fed by our agriculture and food production system. Moreover, currently up to five billion people are already affected by malnutrition including undernutrition, inadequate consumption of micronutrients, and overweight. A healthy and sustainable diet will therefore play a pivotal role in our future, but most food products are traded and consumed based only on techno-functional or gustatory properties. We wish to stimulate a debate on the urgent need for multidisciplinary research and education to realize future diets with enhanced nutritional profiles. In particular, there is a need to better measure and understand the factors that influence the nutrients of food products along the global supply chains.

Artificial intelligence (AI) is advancing rapidly and continually evolving in various fields. Recently, the release of ChatGPT has sparked significant public interest. In this study, we revisit the '100 Important Questions Facing Plant Science' by leveraging ChatGPT as a valuable tool for generating thought-provoking questions relevant to plant science. These questions primarily revolve around the utilization of plants in product development, understanding plant mechanisms, plant-environment interactions, and enhancing plant traits, with an emphasis on sustainable product development. While ChatGPT may not capture certain crucial aspects highlighted by scientists, it offers valuable insights into the questions generated by experts. Our analysis demonstrates that ChatGPT can be cautiously employed as a supportive tool to facilitate, streamline, and expedite specific tasks in plant science.

The importance of biological nitrogen fixation (BNF) in securing food production for the growing world population with minimal environmental cost has been increasingly acknowledged. Leaf surfaces are one of the biggest microbial habitats on Earth, harboring diverse free-living N2-fixers. These microbes inhabit the epiphytic and endophytic phyllosphere and contribute significantly to plant N supply and growth. Here, we summarize the contribution of phyllosphere-BNF to global N cycling, evaluate the diversity of leaf-associated N2-fixers across plant hosts and ecosystems, illustrate the ecological adaptation of N2-fixers to the phyllosphere, and identify the environmental factors driving BNF. Finally, we discuss potential BNF engineering strategies to improve the nitrogen uptake in plant leaves and thus sustainable food production.

Evo-devo is often thought of as being the study of which genes underlie which phenotypes. However, evo-devo is much more than this, especially in plant science. In leaf scars along stems, cell changes across wood growth rings, or flowers along inflorescences, plants trace a record of their own development. Plant morpho evo-devo provides data that genes could never furnish on themes such as heterochrony, the evolution of temporal phenotypes, modularity, and phenotype-first evolution. As plant science surges into increasingly -omic realms, it is essential to keep plant morpho evo-devo in full view as an honored member of the evo-devo canon, ensuring that plant scientists can, wherever they are, generate fundamental insights at the appropriate level of biological organization.

Stomatal pores facilitate gaseous exchange between the inner air spaces of the leaf and the atmosphere. As gatekeepers that balance CO2 entry for photosynthesis against transpirational water loss, they are a focal point for efforts to improve crop performance, especially in the efficiency of water use, within the changing global environment. Until recently, engineering strategies had focused on stomatal conductance in the steady state. These strategies are limited by the physical constraints of CO2 and water exchange such that gains in water-use efficiency (WUE) commonly come at a cost in carbon assimilation. Attention to stomatal speed and responsiveness circumvents these constraints and offers alternatives to enhancing WUE that also promise increases in carbon assimilation in the field.

Seed thermoinhibition protects emerging seedlings from thermodamage by preventing seed germination at elevated temperatures. It had remained unknown how a seed fine-tunes its germination in response to temperature. Recently, Piskurewicz et al. demonstrated that endosperm phyB senses increased temperature, thereby facilitating PIF3-mediated abscisic acid (ABA) accumulation to inhibit germination and embryo elongation.

There is overwhelming evidence that synthetic pesticides have a negative impact on the environment and human health, emphasizing the need for novel and sustainable methods for plant protection. A growing body of literature reports that plants interact through substrate-borne vibrations with arthropod pests and mutualistic arthropods that provide biological control and pollination services. Here, we propose a new theoretical framework that integrates insights from biological control, the ecology of fear, and plant-borne vibrations, to address plant-insect interactions and explore new, sustainable opportunities to improve plant health and productivity.

Key principles pertaining to RNA biology not infrequently have their origins in plant virology. Examples have arisen from studies on viral RNA-intrinsic properties and the infection process from gene expression, replication, movement, and defense evasion to biotechnological applications. Since RNA is at the core of the central dogma in molecular biology, how plant virology assisted in the reinforcement or adaptations of this concept, while at other instances shook up elements of the doctrine, is discussed. Moreover, despite the negative effects of viral diseases in agriculture worldwide, plant viruses can be considered a scientific treasure trove. Today they remain tools of discovery for biotechnology, studying evolution, cell biology, and host-microbe interactions.

Transcription factors (TFs) bind DNA at specific sequences to regulate gene expression. This universal process is achieved via their DNA-binding domain (DBD). In mammals, the vast diversity of DBD structural conformations and the way in which they contact DNA has been used to organize TFs in the TFClass hierarchical classification. However, the numerous DBD types present in plants but absent from mammalian genomes were missing from this classification. We reviewed DBD 3D structures and models available for plant TFs to classify most of the 56 recognized plant TF types within the TFClass framework. This extended classification adds eight new classes and 37 new families corresponding to DBD structures absent in mammals. Plant-TFClass provides a unique resource for TF comparison across families and organisms.

Indigenous and local knowledge (ILK) holders have deep ecological, horticultural, and practical knowledge of plants, but this knowledge is not routinely considered and supported along with seed collections conserved ex situ. In this opinion, conceived collaboratively by a team of botanists, ecologists, ethnobiologists, and practitioners in biodiversity and ILK systems conservation, we propose seven actions towards the co-conservation of seeds and associated knowledge to overcome obstacles and encourage ex situ conservation institutions to support knowledge holders in multiple ways. Success depends on simultaneous changes in conservation practices, new collaborative relationships, and shifts in policy to share and conserve biocultural diversity. Failure to act will witness the continued erosion of ILK and depreciation of ex situ plant collections.

Nematodes are a crucial component of rhizosphere biodiversity, affecting plant health as the most abundant and functionally diverse soil animals. Plant-parasitic nematodes are generally considered harmful, which may overlook their potential benefits to plants when coexisting with free-living nematodes in soil. We provide new insights into nematodes as vital plant partners. Plant root damage by plant-parasitic nematodes creates opportunities for pathogens and beneficial microbiota to colonize the rhizosphere. Free-living nematodes coordinate microbiota to suppress plant diseases, but they are susceptible to mortality from plant pathogens, potentially favoring pathogen release in the root zone. We conclude that the nematode's role in regulating plant pathogens represents a missing link, constraining our ability to predict and control soil-borne diseases in healthy plants.

The plant-associated microbiome is known to be a critical component for crop growth, nutrient acquisition, resistance to pathogens, and abiotic stress tolerance. Conventional approaches have been attempted to manipulate the plant-soil microbiome to improve plant performance; however, several issues have arisen, such as collateral negative impacts on microbiota composition. The lack of reliability and robustness of conventional techniques warrants efforts to develop novel alternative strategies. Nano-enabled approaches have emerged as promising platforms for enhancing agricultural sustainability and global food security. Specifically, the use of engineered nanomaterials (ENMs) as nanoscale agrochemicals has great potential to modulate the plant-associated microbiome. We review the dynamic interplay between nano-agrochemicals and the plant-associated microbiome for the safe development and use of nano-enabled microbiome engineering.

Orphan genes (OGs) are protein-coding genes without a significant sequence similarity in closely related species. Despite their functional importance, very little is known about the underlying molecular mechanisms by which OGs participate in diverse biological processes. Here, we discuss the evolutionary mechanisms of OGs' emergence with relevance to species-specific adaptations. We also provide a mechanistic view of the involvement of OGs in multiple processes, including growth, development, reproduction, and carbon-metabolism-mediated immunity. We highlight the interconnection between OGs and the sucrose nonfermenting 1 (SNF1)-related protein kinases (SnRKs)-target of rapamycin (TOR) signaling axis for phytohormone signaling, nutrient metabolism, and stress responses. Finally, we propose a high-throughput pipeline for OGs' interspecies and intraspecies gene transfer through a transgenic approach for future biotechnological advances.

Histone deacetylases (HDACs) are important chromatin regulators essential for plant tolerance to adverse environments. In addition to histone deacetylation and epigenetic regulation, HDACs deacetylate non-histone proteins and thereby regulate multiple pathways. Like other post-translational modifications (PTMs), acetylation/deacetylation is a reversible switch regulating different cellular processes in plants. Here, by focusing on results obtained in arabidopsis (Arabidopsis thaliana) and rice plants, we analyze the different aspects of HDAC functions and the underlying regulatory mechanisms in modulating plant responses to stress. We hypothesize that, in addition to epigenetic regulation of gene expression, HDACs can also control plant tolerance to stress by regulating transcription, translation, and metabolic activities and possibly assembly-disassembly of stress granules (SGs) through lysine deacetylation of non-histone proteins.

As sessile organisms, plants must overcome various stresses. Accordingly, they have evolved several plant-specific growth and developmental processes. These plant processes may be related to the evolution of plant-specific protein families. The WRKY transcription factors originated in eukaryotes and expanded in plants, but are not present in animals. Over the past two decades, there have been many studies on WRKYs in plants, with much of the research concentrated on their roles in stress responses. Nevertheless, recent findings have revealed that WRKYs are also required for seed dormancy and germination, postembryonic morphogenesis, flowering, gametophyte development, and seed production. Thus, WRKYs may be important for plant adaptations to a sessile lifestyle because they simultaneously regulate stress resistance and plant-specific growth and development.

Freshwater ecosystems are of worldwide importance for maintaining biodiversity and sustaining the provision of a myriad of ecosystem services to modern societies. Plants, one of the most important components of these ecosystems, are key to water nutrient removal, carbon storage, and food provision. Understanding how the functional connection between freshwater plants and ecosystems is affected by global change will be key to our ability to predict future changes in freshwater systems. Here, we synthesize global plant responses, adaptations, and feedbacks to present-day and future freshwater environments through trait-based approaches, from single individuals to entire communities. We outline the transdisciplinary knowledge benchmarks needed to further understand freshwater plant biodiversity and the fundamental services they provide.