Polyamines are small organic and basic polycations that perform essential regulatory functions in all living organisms. Fluctuations in polyamine content have been observed to occur during growth, development and under stress conditions, implying that polyamines play pivotal roles in diverse cellular and physiological processes. To achieve polyamine homeostasis, the entire metabolic pathway is subjected to a fine-tuned regulation of its biosynthetic and catabolic genes and enzymes. In this review, we describe and discuss the most important mechanisms implicated in the translational and post-translational regulation of polyamine metabolic enzymes in plants. At the translational level, we emphasize the role of polyamines in the modulation of upstream open reading frame (uORF) activities that control the translation of polyamine biosynthetic and catabolic mRNAs. At the post-translational level, different aspects of the regulation of polyamine metabolic proteins are depicted, such as the proteolytic activation of enzyme precursors, the importance of dimerization in protein stability as well as in protein intracellular localization.

Bioflocculant may be a promising bioactivator for heavy metal removal duo to its eco-friendly properties and remarkable ability to adsorb heavy metals. In this study, bioflocculant production from a bacterium, Pseudomonas sp. GO2, was optimized and its removal efficiency for two heavy metal ions was evaluated. Results demonstrated that the maximal flocculation efficiency was achieved with concentration levels of 5 g/L glucose, 3 g/L casein, and 5 g/L NaCl, with an initial pH of 9.0, and a fermentation time of 48 h. Bioflocculant produced by GO2 had a stronger removal efficiency for Cd2+ than that of Pb2+, with highest removal efficiencies of 85.38% and 80.87%, respectively. The adsorption process was mainly dependent on the monolayer and chemisorption based on the adsorption isotherm and kinetic models. This study demonstrated that bioflocculant produced by the GO2 strain has the potential to be used in heavy metal treatment from industrial wastewater.

Open sewage contaminated channel wastewater (OSCCW) has high pollutant loads, responsible for eutrophication, when mixed with various channels of urban communities. But, these pollutants can be converted and recovered into useful end products with the help of algal species. In this study, two species of Chlorella (C. vulgaris and C. pyrenoidosa) were selected and investigated for the production of algal biomass and nutrient removal efficiencies with 50% concentration of OSCCW, in a comparative way at lab-scale. Chlorella sp. cultivated in OSCCW have removed nitrate (76.9-78.8%) and phosphate (67.6-79.7%) whereas COD (72.4-76.2%) and BOD (62.3-72.4%) respectively. Correlation analysis was investigated between physico-chemical parameters and biochemical profile of both species to analyze the positive and negative correlation between two variables. The bio-chemical profile and biomass productivity of both species of Chlorella were observed well on the basis of productivity of biomass (60.1, 56.5 mg/l/d), carbohydrate (15.71, 8.82 mg/l/d), protein (11.21, 15.82 mg/l/d), lipid (20.8, 17.5 mg/l/d) and chlorophyll (0.78, 0.67 mg/l/d) in OSCCW. The maximum lipid content (34.6%) was obtained with C. pyrenoidosa as compared to C. vulgaris. Findings also support that OSCCW is well-off with nutrient resources, which can be suitable alternative for algal biomass production and remediated wastewater can be used for animal and fish farming type activities.

Biogas production by anaerobic digestion (AD) of organic wastes is important for the transition to fossil free fuels in both the transport sector, industries and shipping. The aim of this study was to target the residual organic matter in the outgoing residue from the AD process, so called digestate, with different thermal treatment methods in order to improve digestate degradability and biogas potential upon post-digestion. The thermal treatment was performed at 55 °C in 24 h, 70 °C in 1 h and by thermal hydrolysis process (THP; 165 °C, 8 bar in 0.33 h), and were carefully selected to offer a simultaneous possibility for pasteurization of the digestate according to the regulations in Sweden. Digestates from ten full-scale biogas plants were collected, with different substrate profiles including wastewater treatment plant (WWTP), food waste digestion, agriculture digestion and manure digestion. The results showed that all thermal treatment methods caused increased dissolved organic carbon concentration (DOC). Four of the thermal treated digestates with the highest increase in DOC were subsequently tested for the bio-methane potential. Thermal treatments at 70 °C and THP, respectively, resulted in the highest increase in bio-methane potentials, with an increase of 15-39% for one WWTP, 38 - 40% for digestate from an agriculture digestion plant and 20 - 22% for digestate from a co-digestion plant treating food waste. Interestingly, the bio-methane potential from digestate treated with the energy-intense THP method, did not show any significant difference compared to thermal treatment at 70 °C for 1 h. The outcomes of this study suggest that placing a pasteurization unit between a main digester and a post digester, when applying two-step digestion allows for a combined pasteurization and increased biogas production.

Supply and uptake of amino acids is of great importance to mammalian cell culture processes. Mammalian cells such as Chinese hamster ovary (CHO) cells express several amino acid (AA) transporters including uniporters and exchangers. Each transporter transports multiple AAs, making prediction of the effect of changed medium composition or transporter levels on individual AA transport rate challenging. A general kinetic model for such combinatorial amino acid transport, and a simplified analytical expression for the uptake rate as a function of amino acid concentrations and transporter levels is presented. From this general model, a CHO cell-specific AA transport model, to our knowledge the first such network model for any cell type, is constructed. The model is validated by its prediction of reported uptake flux and dependencies from experiments that were not used in model construction or parameter estimation. The model defines theoretical conditions for synergistic/repressive effect on the uptake rates of other AAs upon external addition of one AA. The ability of the CHO-specific model to predict amino acid interdependencies experimentally observed in other mammalian cell types suggests its robustness. This model will help formulate testable hypotheses of the effect of process changes on AA initial uptake, and serve as the AA transport component of kinetic models for cellular metabolism.

The present work was conducted to investigate the immobilization of lipase from Penicillium sp. onto three modified bentonites by simple adsorption and crosslinking methods. The composites were characterized by FTIR, SEM and BET. The free and bentonite-supported lipase was evaluated in terms of operational and storage stability and pH and thermal activity and stability. The kinetic parameters were also evaluated. The results show that all immobilized enzymes had better thermal and pH stability compared to free enzymes. Among the immobilized enzymes, GDU-bent-lipase had more efficient performance in thermal (38% of its initial activity within 24 h at 65 °C), operational (70% residual activity after 9 cycles), storage stability (70.14% of its initial activities at 4 °C for 21 days), and kinetic properties (effectiveness factor 0.79 relative to free enzyme) than free and other immobilized enzymes. The adsorption isotherm was modeled by Langmuir, Freundlich and Temkin isotherms which Langmuir isotherm indicated a better fit of the experimental adsorption data. To the best of our knowledge, this is the first comparative report about the immobilization of lipase Produced by Penicillium sp., isolated from olive mill wastewater, and the most comprehensive study about the immobilization of lipase onto several supports.

Several forms of EcaA protein, correspondent to the extracellular α-class carbonic anhydrase (CA) of cyanobacterium Crocosphaera subtropica ATCC 51142 were expressed in Escherichia coli. The recombinant proteins with no leader peptide (EcaA and its fusion with thioredoxin or glutathione S-transferase) were allocated inside cells in a full-length form; these cells did not display any extracellular CA activity. Soluble proteins (including that of periplasmic space) of E. coli cells that expressed both ЕсаА equipped with its native leader peptide (L-EcaA) as well as L-EcaA fused with thioredoxin or glutathione S-transferase at N-terminus, mainly contained the processed EcaA. The appearance of mature ЕсаА in outer layers of E. coli cells expressed leader peptide-containing forms of recombinant proteins, has been directly confirmed by immunofluorescent microscopy. Those cells also displayed high extracellular CA activity. In addition, the mature EcaA protein was detected in the culture medium. This suggests that cyanobacterial signal peptide is recognized by the secretory machinery and by the leader peptidase of E. coli even as a part of a fusion protein. The efficiency of EcaA leader peptide was comparable to that of PelB and TorA signal peptides, commonly used for biotechnological production of extracellular recombinant proteins in E. coli.

Co-culture of microalgae and microorganisms, supported with the resulting synergistic effects, can be used for wastewater treatment, biomass production, agricultural applications and etc. Therefore, this study aimed to explore the role of Bacillus subtilis (B. subtilis) in tolerance against the harsh environment of seafood wastewater, at which these microalgal-bacterial flocs were formed by microalgae cultivation. In this present study, B. subtilis isolated from the cultivation medium of Chlorella vulgaris and exposed to different salinity (0.1-4% w/v sodium chloride) and various pH range to determine the tolerant ability and biofilm formation. Interestingly, this bacteria strain that isolated from microalgae cultivation medium showed the intense viability in the salt concentration exceeding up to 4% (w/v) NaCl but demonstrated the decrease in cell division as environmental culture undergoing over pH 10. Cell viability was recorded higher than 71% and 92% for B. subtilis inoculum in media with salt concentration greater than 20 gL-1 and external pH 6.5-9, respectively. This showed that B. subtilis isolated from microalgal-bacteria cocultivation exhibited its tolerant ability to survive in the extremely harsh conditions and thus, mitigating the stresses due to salinity and pH.

Burgeoning human population dents, globally, the brimming buffer stock as well as gain in food grain production. However, an imminent global starvation was averted through precise scientific intervention and pragmatic policy changes in the 1960s and was eulogized as the "Green Revolution". Miracle rice and wheat obtained through morphometric changes in the ideotype of these two crops yielded bumper harvest that nucleated in Asia and translated into Latin America. The altered agronomic traits in these two crops were the result of tinkering with the phyto-hormone "Gibberellin'. Recently, another plant hormone 'Cytokinin' has gained prominence for its involvement in the grain revolution in rice and other field crops. Suo moto homeostasis of CK by the cytokinin oxidase enzyme governs the cardinal shoot apical meristem that produces new flowering primordia thereby enhancing grain number. Similarly, the flowering hormone 'Florigen' impacts sympodia formation, flowering, and fruit production in tomato. The role of heterozygosity induced heterosis by florigen in revolutionizing tomato production and cellular homeostasis of CK by CK oxidising enzyme (CKX) in enhancing rice production has been path-breaking. This review highlights role of phytohormones in grain revolution and crop specific fine-tuning of gibberellins, cytokinins and florigen to accomplish maximum yield potential in field crops.

Locusta migratoria apolipophorin III (apoLp-III) possesses the ability to exist as a water soluble amphipathic α-helix bundle and a lipid surface seeking apolipoprotein. The intrinsic ability of apoLp-III to transform phospholipid vesicles into reconstituted discoidal high-density lipoproteins (rHDL) has led to myriad applications. To improve the yield of recombinant apoLp-III, studies were performed in a bioreactor. Induction of apoLp-III expression generated a protein product that is secreted from E. coli into the culture medium. Interaction of apoLp-III with gas and liquid components in media produced large quantities of thick foam. A continuous foam fractionation process yielded a foamate containing apoLp-III as the sole major protein component. The yield of recombinant apoLp-III was ~0.2 g / liter bacterial culture. Mass spectrometry analysis verified the identity of the target protein and indicated no modifications or changes to apoLp-III occurred as a result of foam fractionation. The functional ability of apoLp-III to induce rHDL formation was evaluated by incubating foam fractionated apoLp-III with phosphatidylcholine vesicles. FPLC size exclusion chromatography revealed a single major population of particles in the size range of rHDL. The results described offer a novel approach to bioreactor-based apoLp-III production that takes advantage of its intrinsic biosurfactant properties.

Over the past decade, formic acid and acetic acid have gained increasing attention as alternative feedstocks for poly-3-hydroxybutyrate (PHB) production as these potentially CO2-derived molecules are naturally assimilated by Cupriavidus necator. Both organic acids were individually evaluated in fed-batch fermentations at bioreactor scale. Acetic acid was revealed as the most promising carbon source yielding 42.3 g L-1 PHB, whereas no significant amount of PHB was produced from formic acid. Hence, acetic acid was further used as the substrate during process intensification. Key performance characteristics, including process stability, PHB titer, and productivity were optimized by introducing NH4-acetate as the nitrogen source, extending the growth phase, and implementing a repeated fed-batch procedure, respectively. These advanced fermentation strategies resulted in the establishment of a stable fermentation process reaching 58.5 g L-1 PHB, while doubling the productivity to 0.93 g L-1 h-1 PHB.

Impedance spectroscopy is a technique used to characterize electrochemical systems, increasing its applicability as well to monitor cell cultures. During their growth, Bacillus species have different phases which involve the production and consumption of different metabolites, culminating in the cell differentiation process that allows the generation of bacterial spores. In order to use impedance spectroscopy as a tool to monitor industrial interest Bacillus cultures, we conducted batch fermentations of Bacillus species such as B. subtilis, B. amyloliquefaciens, and B. licheniformis coupled with this technique. Each fermentation was characterized by the scanning of 50 frequencies between 0.5 and 5 MHz every 30 min. Pearson's correlation between impedance and phase angle profiles (obtained from each frequency scanned) with the kinetic profiles of each strain allowed the selection of fixed frequencies of 0.5, 1.143, and 1.878 MHz to follow-up of the fermentations of B. subtilis, B. amyloliquefaciens and B. licheniformis, respectively. Dielectric profiles of impedance, phase angle, reactance, and resistance obtained at the fixed frequency showed consistent changes with exponential, transition, and spore release phases.

Herein, we report the synthesis of taurine incorporated (sulfur containing organic molecule derived from methionine and cysteine) hybrid nanoflowers (thNFs) with an intrinsic peroxidase-mimic and antimicrobial activities in the presence of H2O2. Formation of thNFs using non-enzyme molecules was for the first time and systematically studied as a function of the taurine concentration, types of metal ions (Cu2+, Fe2+ and Fe3+) and pH values of reaction solution. The peroxidase like activities of thNFs rely on Fenton-like reaction against guaiacol used as a model substrate. The efficiency of Fenton reaction can be attributed to porous structure and presence of ions of transition elements in the thNFs. The thNFs were further characterized using FTIR, XRD, SEM and EDX. The thNFs also showed remarkable antimicrobial properties against S. aureus, E. coli, B. cereus and C. albicans. We claim that nonprotein-based NFs can be considered as new generation nano-biocatalysts as an alternative to enzymes and can be used in various medicinal, biochemical, immunological, biotechnological, and industrial applications.

Methanol is a promising green feedstock for producing fuels and chemicals because it is inexpensive, clean, environmentally friendly, and easily prepared. Thus, many studies have been devoted to engineering non-native methylotrophic platform microorganisms to utilize methanol. This study adopted a series of strategies to develop a synthetic methylotrophic Bacillus subtilis that can use methanol as the carbon source, including the heterologous expression of methanol dehydrogenase (Mdh), enhancement of the expressions of 3-hexulose-6-phosphate synthase (Hps) and 6-phospho-3-hexuloisomerase (Phi), regulation of the expressions of key enzymes at both the translational and transcriptional levels, stabilization of the key enzyme expression through a dual-system for expressing the target genes on both the plasmid and genome, and improvement of the catalytic activity of Mdh with a recycling strategy for NAD+. As a result, the methanol consumption of the synthetic methylotrophic B. subtilis reached 4.09 g/L, with the maximum OD600 showing a 2.21-fold increase compared with the wild-type B. subtilis, which cannot use methanol. We further deleted the phosphoglucose isomerase (Pgi) and added co-substrates to increase the supply of ribulose-5-phosphate (Ru-5-P), and the specific methanol consumption rate increased by an additional 27.54%. Finally, we successfully constructed two strains that cannot grow in M9 medium with xylose or ribose unless methanol is utilized. The strategies used in this study are generally applicable to other studies on synthetic methylotrophy.

The industrial effluent contaminated with organic pollutants has been causing an increase in the toxicity of the ecosystem, causing a great environmental impact. Thus, the present work aims the green synthesis of silver nanoparticles (AgNPs) from Aloe vera, its characterization and antimicrobial activity against Pseudomonas aeruginosa (ATCC 27853) and Staphylococcus aureus (ATCC 25923). AgNPs were characterized by X-ray diffraction (XRD), Scanning Electronic Microscopy with Energy Dispersive Spectroscopy (SEM-EDS), Zeta Potential (ZP) and N2 porosimetry (BET/BJH method). Antimicrobial activity were carried out by Minimal Inhibitory Concentration (MIC) method. The XRD demonstrated characteristic peaks of AgNPs at 38.29°; 44.55° and 64.81°, and SEM-EDS micrographs showed that AgNPs produced by biomolecules of Aloe vera extract resulted in a weight concentration around 92.59% silver, 7.15% oxygen and 0.26% chlorine. Regarding zeta potential, all samples showed negative electric charge (around -35.3 mV), while N2 porosimetry resulted in a surface specific area of 6.09 m2 g-1, with a volume and diameter pore of 0.032 cm³ g-1 and 33.47, respectively. Antimicrobial activity was observed at 15.62 µg mL-1 and 31.25 µg mL-1 for P. aeruginosa and S. aureus, respectively. Thus, AgNPs can be considered a promising nanoparticle for degradation of organic pollutants in aqueous solution as well as an adjuvant for treatment of microbial infections.

Presence of methanotrophs in diverse environmental habitats helps to reduce emissions of greenhouse gas like methane. Isolation and culture of undiscovered wealth of methanotrophic organisms can help in exploitation of these organisms in value added products. The present study focuses on the enrichment of methanotroph dominated mixed microbial community by use of three stage strategy of revival, proliferation, and segregation. During the enrichment process amplicon sequencing of 16 s rRNA V3-V4 region showed relative abundance of mixed culture comprising single methanotrophic species of Methylocystis genus (88.92%) along with only three other species. Methylocystis dominant mixed culture (MMI-11) was observed to produce polyhydroxyalkanoates (PHA). During studies to identify favourable culture conditions, nitrate was found to be preferred nitrogen source for growth and PHA production. Cell growth ability to produce PHA was also evaluated at 14 L fermentor by supplying gas using continuous bubbling and through pressurization in the headspace. The mixed methanotrophic culture was found to accumulate maximum of 22.20% polyhydroxybutyrate (PHB) under nitrate limited condition. The molecular weight of PHB was found to be 2.221 × 105 g mol-1 with polydispersity of 1.82.

Transaminases catalyze the transfer of an amino group from a donor to a keto group of an acceptor substrate and are applicable to the asymmetric synthesis of herbicide L-phosphinothricin (L-PPT). Here, the important residue sites (C390, I22, V52, R141, Y138 and D239) of transaminase from Salmonella enterica (SeTA) were modified at the adjacency of the substrate-binding pocket to improve the enzyme activity. Among the constructed mutant library, the SeTA-Y138F mutant displayed higher activity than the wild-type enzyme. Compared to the wild-type, SeTA-Y138F showed improved catalytic efficiency with a 4.36-fold increase. The Km and kcat of SeTA -Y138F toward 4-(hydroxy(methyl) phosphoryl)-2-oxobutanoic acid (PPO) were 26.39 mM and 34.28 s-1, respectively. Subsequently, the three-enzyme co-expression system of E. coli BL21 (DE3)/pACYCDuet-SeTA-Y138F/pETDuet-AlaDH-BsGDH was developed by combining a alanine dehydrogenase (AlaDH) to recycle the byproduct of amino donor, a glucose dehydrogenase (BsGDH) for cofactor recycling. Under the optimized conditions, an excellent L-PPT yield of 90.8% was achieved by the whole-cell biotransformation with 500 mM PPO. It exhibited the tri-enzymatic coupling system was potential for effective production of target L-PPT.

Phenolic aldehydes are the major inhibitors from lignocellulose pretreatment. Previous studies show that oleaginous yeasts are difficult to survive in lignocellulosic hydrolysates even after the removal of furan aldehydes and organic acids inhibitors. This study investigated the cell viability, sugar consumption and lipid accumulation of the major oleaginous yeasts including Trichosporon cutaneum, Rhodosporidium toruloides, Rhodotorula glutinis, Yarrowia lipolytica in wheat straw hydrolysate containing only phenolic aldehydes after furan aldehydes and organic acids were selectively degraded by microorganisms. The results confirmed that the existence of residual phenolic aldehydes was the major reason for poor cell growth and metabolism of oleaginous yeasts. Only T. cutaneum demonstrated the higher tolerance by biodegrading phenolic aldehydes and the satisfactory cell growth and lipid production were obtained. This study revealed that T. cutaneum might be one of the promising cell factories for microbial lipid production from lignocellulosic feedstock.

Diatoms are currently considered valuable feedstocks for different biotechnological applications. To deepen the knowledge on the production of these microalgae, the diel pattern of batch growth, photosystem II performance, and accumulation of target metabolites of two commercially relevant diatoms, Phaeodactylum tricornutum and Skeletonema costatum, were followed outdoors in 100-L flat panel photobioreactors. S. costatum presented a higher light-to-biomass conversion resulting in higher growth than P. tricornutum. Both fluorescence data and principal component analysis pointed to temperature as a limiting factor for the growth of P. tricornutum. Higher protein and carbohydrate contents were found in P. tricornutum, whereas S. costatum fatty acids were characterized by a higher unsaturation degree. Higher productivities were found at 1 p.m. for protein, lipid, and ash in the case of S. costatum. Overall, S. costatum showed great potential for outdoor cultivation, revealing a broader temperature tolerance and increased biomass productivity than P. tricornutum.

A comparative study of existing junction-primer-designing software revealed many limitations among them. Hence, we developed a new computational program, Ex-Ex Primer, which offers many improved, user-friendly features, and reliably creates junction primers and probes. This online suite can also be used to design primers/probes from other sites of nucleic acid recombination, insertion, deletion, or splicing, and regular probes/primers. The threshold for Tm difference between the complete junctional primer vs its partial sequence, which maps to one of the junctional regions, was changed based on an important observation made during the initial experimental validations. The tool is now thoroughly checked with RT-PCR and RT-qPCR experiments with more than 250 primer pairs over a few years. The junction-primer-designing features of the software are also better than other equivalent tools. Visualizing the exons and introns across transcripts, and enabling primer designing based on information from Ensembl, are some of the unique features of this tool. The primers suggested by the tool can be used to detect the expression of known transcripts, to test the existence of predicted DNA or RNA joints via hybridization-based techniques, or for validation and in silico analysis of RNA-Seq. URL: http://resource2.ibab.ac.in/exprimer/.

As a common phenomenon existing in almost all genomes, codon usage bias has been studied for a long time. Codon optimization is a frequently used strategy to accelerate protein synthesis rate. Besides regulating protein translation speed, codon usage bias has also be reported to affect co-translation folding and transcription. P. pastoris is a well-developed expression system, whose efficiency is tightly correlated with commercial value. However, few studies focus on the role of codon usage bias in affecting protein expression in P. pastoris. Besides, many genes in P. pastoris genome show significant negative correlation between codon usage bias and protein structural disorder tendency. It's not known whether this feature is important for their expression. In order to answer these questions, we picked 4P. pastoris gene candidates with strong negative correlation between codon usage bias and protein structural disorder. We then performed full-length codon optimization which completely eliminated the correlation. Protein and RNA assays were then used to compare protein and mRNA levels before and after codon optimization. As a result, codon optimization failed to elevate their protein expression levels, and even resulted in a decrease. As represented by the trypsin sensitivity assays, codon optimization also altered the protein structure of 0616 and 0788. Besides protein, codon optimization also affected mRNA levels. Shown by in vitro and in vivo RNA degradation assays, the mRNA stability of 0616, 0788 and 0135 were also altered by codon optimization. For each gene, the detailed effect may be related with its specific sequence and protein structure. Our results suggest that codon usage bias is an important factor to regulate gene expression level, as well as mRNA and protein stabilities in P. pastoris. "Extreme" codon optimization in genes with strong negative correlation between codon usage bias and protein structural disorder tendency may not be favored. Compromised strategies should be tried if expression is not successful. Besides, codon optimization may affect protein structural conformation more severely in structural disordered proteins.

The present study investigates ameliorative effect of silicon nanoparticles (SiNPs) and indole acetic acid (IAA) alone and in combination against hexavalent chromium (CrVI) toxicity in rice seedlings. The results of the study revealed protective effects of SiNPs and IAA against CrVI toxicity. The 100 μM of CrVI imposed toxic effects in rice seedlings at morphological, physiological and biochemical levels which coincided with increased level of intracellular CrVI and declined level of endogenous nitric oxide (NO). The CrVI enhanced levels of superoxide radicals (SOR) (59.51% and 50.1% in shoot and root, respectively) and H2O2 (19.5% and 23.69% in shoot and root, respectively). However, when SiNPs and IAA were applied to plants under CrVI stress, they enhanced tolerance and defence mechanisms as manifested in terms of increased biomass, endogenous NO, photosynthetic pigments, and antioxidants level. It was also noticed that CrVI arrested cell cycle at G2/M phase whereas growth was restored as compared to control when SiNPs and IAA were supplemented. Thus, the hypothesis that combined application of SiNPs and IAA will be effective in alleviating CrVI toxicity is validated from the results of this study. Moreover, in SiNPs and IAA-mediated mitigation of CrVI toxicity, endogenous NO has a positive role. The importance of the study will be that the combination of SiNPs and IAA can be utilized against heavy metal stress and even when supplied alone, they will enhance the crop productivity parameters with and without stress conditions.

Beginning of industrialization accelerates the heavy metal pollution in the biosphere. Plant being the immovable entity utilizes different mechanisms to flee from unfavourable conditions. To alleviate toxic impact of metals like cadmium (Cd) and lead (Pb), phytohormones such as indole acetic acid (IAA) has been applied exogenously. This manuscript aims to evaluate the significant change occurring in biochemical parameters of Indian mustard (Brassica napus) grown under individual and combined treatments of IAA with Cd and Pb. Herbicidal potential of treated Brassica extracts were evaluated on growth and development of Anagallis arvensis. Quantum yield parameters were more sensitive to Cd than Pb stress resulted in reduced photosynthetic pigments. However, exogenously applied IAA together with Cd and Pb considerably improved the level of photosynthetic attributes along with reduced accumulation of Cd and Pb in Brassica plant. Cd and Pb enhanced the activities of reactive oxygen species (ROS) and antioxidant machinery. However, addition of IAA with Cd and Pb mitigated the effect of heavy metals on antioxidant system. Moreover, activity of the phenylalanine ammonia lyase enzyme and the defensive metabolites (phenolic, flavonoid and anthocyanin compounds) were boosted under individual treatments of Cd and Pb responsible for increasing herbicidal potential of Brassica plant. Our results exhibited essentiality of IAA in mitigating Cd and Pb stress in Brassica through up-regulated mechanisms of the antioxidant system for balancing ROS related injuries. Increased metabolites enhancing herbicidal potential of Brassica napus against Anagallis weed were also observed.