生信网-文献期刊

Int J Mol Sci

2021 Nov 9

Wang N, Ji X, Wu Y

2. Developmental Toxicology of Metal Mixtures in Drosophila: Unique Properties of Potency and Interactions of Mercury Isoforms.

Int J Mol Sci

2021 Nov 9

Beamish CR, Love TM, Rand MD.

3. Effects of Cynaroside on Cell Proliferation, Apoptosis, Migration and Invasion though the MET/AKT/mTOR Axis in Gastric Cancer.

Int J Mol Sci

2021 Nov 9

Ji J, Wang Z, Sun W

4. Experimental Models, Induction Protocols, and Measured Parameters in Dry Eye Disease: Focusing on Practical Implications for Experimental Research.

Int J Mol Sci

2021 Nov 9

Rahman MM, Kim DH, Park CK

5. The Medial Prefrontal Cortex and Fear Memory: Dynamics, Connectivity, and Engrams.

Int J Mol Sci

2021 Nov 9

Dixsaut L, Gräff J.

6. Discovery of Phenolic Glycoside from Hyssopus cuspidatus Attenuates LPS-Induced Inflammatory Responses by Inhibition of iNOS and COX-2 Expression through Suppression of NF-κB Activation.

Int J Mol Sci

2021 Nov 9

Liu X, Su J, Wang G

It seems quite necessary to obtain effective substances from natural products against inflammatory response (IR) as there are presently clinical problems regarding accompanying side effects and lowered quality of life. This work aimed to investigate the abilities of hyssopuside (HY), a novel phenolic glycoside isolated from Hyssopus cuspidatus (H. cuspidatus), against IR in lipopolysaccharide (LPS)-induced RAW 264.7 cells and mouse peritoneal macrophages. The results indicated that HY could reduce nitric oxide (NO) production and inhibit the production and secretion of pro-inflammatory mediators including tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β) in LPS-stimulated macrophages. Moreover, data from the immunofluorescence study showed that HY suppressed nuclear translocation of nuclear factor-kappa B (NF-κB) upon LPS induction. The Western blot results suggested that HY reversed the LPS-induced degradation of IκB (inhibitor of NF-κB), which is normally required for the activation of NF-κB. Meanwhile, the overexpression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) diminished significantly with the presence of HY in response to LPS stimulation. On the other hand, HY had a negligible impact on the activation of mitogen-activated protein kinase (MAPK) pathways. Moreover, an in silico study of HY against four essential proteins/enzymes revealed that COX-2 was the most efficient enzyme for the interaction, and binding of residues Phe179, Asn351, and Ser424 with HY played crucial roles in the observed activity. The structure analysis indicated the typical characterizations with phenylethanoid glycoside contributed to the anti-inflammatory effects of HY. These results indicated that HY manipulated its anti-inflammatory effects mainly through blocking the NF-κB signal transduction pathways. Collectively, we believe that HY could be a potential alternative phenolic agent for alleviating excessive inflammation in many inflammation-associated diseases.

7. MVP Expression Facilitates Tumor Cell Proliferation and Migration Supporting the Metastasis of Colorectal Cancer Cells.

Int J Mol Sci

2021 Nov 9

Pietras P, Leśniczak-Staszak M, Kasprzak A

8. New Drug Targets to Prevent Death Due to Stroke: A Review Based on Results of Protein-Protein Interaction Network, Enrichment, and Annotation Analyses.

Int J Mol Sci

2021 Nov 9

Maes M, Nikiforov NG, Plaimas K

9. The Escherichia coli Outer Membrane β-Barrel Assembly Machinery (BAM) Crosstalks with the Divisome.

Int J Mol Sci

2021 Nov 9

Consoli E, Luirink J, den Blaauwen T.

10. Arginase 2 and Polyamines in Human Pancreatic Beta Cells: Possible Role in the Pathogenesis of Type 2 Diabetes.

Int J Mol Sci

2021 Nov 9

Marselli L, Bosi E, De Luca C

11. Focus on the Small GTPase Rab1: A Key Player in the Pathogenesis of Parkinson's Disease.

Int J Mol Sci

2021 Nov 8

Martínez-Menárguez JÁ, Martínez-Alonso E, Cara-Esteban M

12. Senolytics: A Novel Strategy for Neuroprotection in ALS?

Int J Mol Sci

2021 Nov 8

Maximova A, Werry EL, Kassiou M.

13. Prediction of Peptide Detectability Based on CapsNet and Convolutional Block Attention Module.

Int J Mol Sci

2021 Nov 8

Yu M, Duan Y, Li Z

14. AMBRA1 Negatively Regulates the Function of ALDH1B1, a Cancer Stem Cell Marker, by Controlling Its Ubiquitination.

Int J Mol Sci

2021 Nov 8

Baek SH, Jang YK.

15. Multifunctional Nanomaterials: Synthesis, Properties and Applications.

Int J Mol Sci

2021 Nov 8

Yadav RS.

16. Clioquinol Decreases Levels of Phosphorylated, Truncated, and Oligomerized Tau Protein.

Int J Mol Sci

2021 Nov 8

Lin G, Zhu F, Kanaan NM

The neuropathological hallmarks of Alzheimer's disease (AD) are senile plaques (SPs), which are composed of amyloid β protein (Aβ), and neurofibrillary tangles (NFTs), which consist of highly phosphorylated tau protein. As bio-metal imbalance may be involved in the formation of NFT and SPs, metal regulation may be a direction for AD treatment. Clioquinol (CQ) is a metal-protein attenuating compound with mild chelating effects for Zn2+ and Cu2+, and CQ can not only detach metals from SPs, but also decrease amyloid aggregation in the brain. Previous studies suggested that Cu2+ induces the hyperphosphorylation of tau. However, the effects of CQ on tau were not fully explored. To examine the effects of CQ on tau metabolism, we used a human neuroblastoma cell line, M1C cells, which express wild-type tau protein (4R0N) via tetracycline-off (TetOff) induction. In a morphological study and ATP assay, up to 10 μM CQ had no effect on cell viability; however, 100 μM CQ had cytotoxic effects. CQ decreased accumulation of Cu+ in the M1C cells (39.4% of the control), and both total and phosphorylated tau protein. It also decreased the activity of c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (p38 MAPK) (37.3% and 60.7% levels of the control, respectively), which are tau kinases. Of note, activation of protein phosphatase 2A (PP2A), which is a tau phosphatase, was also observed after CQ treatment. Fractionation experiments demonstrated a reduction of oligomeric tau in the tris insoluble, sarkosyl soluble fraction by CQ treatment. CQ also decreased caspase-cleaved tau, which accelerated the aggregation of tau protein. CQ activated autophagy and proteasome pathways, which are considered important for the degradation of tau protein. Although further studies are needed to elucidate the mechanisms responsible for the effects of CQ on tau, CQ may shed light on possible AD therapeutics.

17. Cubic Octa-Carbon: Quantum-Chemical Design of Molecular Structure and Potential Way of Its Synthesis from Cubane.

Int J Mol Sci

2021 Nov 8

Chachkov DV, Mikhailov OV.

18. Early Deconditioning of Human Skeletal Muscles and the Effects of a Thigh Cuff Countermeasure.

Int J Mol Sci

2021 Nov 8

Fovet T, Guilhot C, Stevens L

19. A Novel Isogenic Human Cell-Based System for MEN1 Syndrome Generated by CRISPR/Cas9 Genome Editing.

Int J Mol Sci

2021 Nov 8

Klementieva N, Goliusova D, Krupinova J

20. B6 Mouse Strain: The Best Fit for LPS-Induced Interstitial Cystitis Model.

Int J Mol Sci

2021 Nov 8

Chen CH, Liao CH, Chen KC

21. Multi-Omics Study of Keystone Species in a Cystic Fibrosis Microbiome.

Int J Mol Sci

2021 Nov 7

Silveira CB, Cobián-Güemes AG, Uranga C

Ecological networking and in vitro studies predict that anaerobic, mucus-degrading bacteria are keystone species in cystic fibrosis (CF) microbiomes. The metabolic byproducts from these bacteria facilitate the colonization and growth of CF pathogens like Pseudomonas aeruginosa. Here, a multi-omics study informed the control of putative anaerobic keystone species during a transition in antibiotic therapy of a CF patient. A quantitative metagenomics approach combining sequence data with epifluorescence microscopy showed that during periods of rapid lung function loss, the patient's lung microbiome was dominated by the anaerobic, mucus-degrading bacteria belonging to Streptococcus, Veillonella, and Prevotella genera. Untargeted metabolomics and community cultures identified high rates of fermentation in these sputa, with the accumulation of lactic acid, citric acid, and acetic acid. P. aeruginosa utilized these fermentation products for growth, as indicated by quantitative transcriptomics data. Transcription levels of P. aeruginosa genes for the utilization of fermentation products were proportional to the abundance of anaerobic bacteria. Clindamycin therapy targeting Gram-positive anaerobes rapidly suppressed anaerobic bacteria and the accumulation of fermentation products. Clindamycin also lowered the abundance and transcription of P. aeruginosa, even though this patient's strain was resistant to this antibiotic. The treatment stabilized the patient's lung function and improved respiratory health for two months, lengthening by a factor of four the between-hospitalization time for this patient. Killing anaerobes indirectly limited the growth of P. aeruginosa by disrupting the cross-feeding of fermentation products. This case study supports the hypothesis that facultative anaerobes operated as keystone species in this CF microbiome. Personalized multi-omics may become a viable approach for routine clinical diagnostics in the future, providing critical information to inform treatment decisions.

22. An Association between Diet and MC4R Genetic Polymorphism, in Relation to Obesity and Metabolic Parameters-A Cross Sectional Population-Based Study.

Int J Mol Sci

2021 Nov 7

Adamska-Patruno E, Bauer W, Bielska D

23. A Small Step, a Giant Leap: Somatic Hypermutation of a Single Amino Acid Leads to Anti-La Autoreactivity.

Int J Mol Sci

2021 Nov 7

Bartsch T, Arndt C, Loureiro LR

24. MALDI-MS Analysis of Peptide Libraries Expands the Scope of Substrates for Farnesyltransferase.

Int J Mol Sci

2021 Nov 7

Schey GL, Buttery PH, Hildebrandt ER

Protein farnesylation is a post-translational modification where a 15-carbon farnesyl isoprenoid is appended to the C-terminal end of a protein by farnesyltransferase (FTase). This modification typically causes proteins to associate with the membrane and allows them to participate in signaling pathways. In the canonical understanding of FTase, the isoprenoids are attached to the cysteine residue of a four-amino-acid CaaX box sequence. However, recent work has shown that five-amino-acid sequences can be recognized, including the pentapeptide CMIIM. This paper describes a new systematic approach to discover novel peptide substrates for FTase by combining the combinatorial power of solid-phase peptide synthesis (SPPS) with the ease of matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS). The workflow consists of synthesizing focused libraries containing 10-20 sequences obtained by randomizing a synthetic peptide at a single position. Incubation of the library with FTase and farnesyl pyrophosphate (FPP) followed by mass spectrometric analysis allows the enzymatic products to be clearly resolved from starting peptides due to the increase in mass that occurs upon farnesylation. Using this method, 30 hits were obtained from a series of libraries containing a total of 80 members. Eight of the above peptides were selected for further evaluation, reflecting a mixture that represented a sampling of diverse substrate space. Six of these sequences were found to be bona fide substrates for FTase, with several meeting or surpassing the in vitro efficiency of the benchmark sequence CMIIM. Experiments in yeast demonstrated that proteins bearing these sequences can be efficiently farnesylated within live cells. Additionally, a bioinformatics search showed that a variety of pentapeptide CaaaX sequences can be found in the mammalian genome, and several of these sequences display excellent farnesylation in vitro and in yeast cells, suggesting that the number of farnesylated proteins within mammalian cells may be larger than previously thought.

25. Heparin and Derivatives for Advanced Cell Therapies.

Int J Mol Sci

2021 Nov 7

Laner-Plamberger S, Oeller M, Rohde E

26. Leaf Apoplast of Field-Grown Potato Analyzed by Quantitative Proteomics and Activity-Based Protein Profiling.

Int J Mol Sci

2021 Nov 6

Abreha KB, Alexandersson E, Resjö S

27. Effects of Arachidonic Acid Metabolites on Cardiovascular Health and Disease.

Int J Mol Sci

2021 Nov 6

Zhou Y, Khan H, Xiao J

28. Cationic Peptides and Their Cu(II) and Ni(II) Complexes: Coordination and Biological Characteristics.

Int J Mol Sci

2021 Nov 6

Kotynia A, Wiatrak B, Kamysz W

29. Development and Validation of Tumor Immunogenicity Based Gene Signature for Skin Cancer Risk Stratification.

Int J Mol Sci

2021 Nov 6

Yavartanoo M, Yi GS.

30. Stem Cell Therapy Enhances Motor Activity of Triceps Surae Muscle in Mice with Hereditary Peripheral Neuropathy.

Int J Mol Sci

2021 Nov 6

Govbakh I, Kyryk V, Ustymenko A

31. Adaptive Responses of Citrus grandis Leaves to Copper Toxicity Revealed by RNA-Seq and Physiology.

Int J Mol Sci

2021 Nov 6

Wu F, Huang H, Peng M

32. The Effect of Glycerin Content in Sodium Alginate/Poly(vinyl alcohol)-Based Hydrogels for Wound Dressing Application.

Int J Mol Sci

2021 Nov 6

Bialik-Wąs K, Pluta K, Malina D

33. Anti-Inflammatory Activity of Three Triterpene from Hippophae rhamnoides L. in Lipopolysaccharide-Stimulated RAW264.7 Cells.

Int J Mol Sci

2021 Nov 5

Han Y, Yuan C, Zhou X

34. Inhibitors of the Sec61 Complex and Novel High Throughput Screening Strategies to Target the Protein Translocation Pathway.

Int J Mol Sci

2021 Nov 5

Pauwels E, Schülein R, Vermeire K.

35. Novel Nanobiocomposites Based on Natural Polysaccharides as Universal Trophic Low-Dose Micronutrients.

Int J Mol Sci

2021 Nov 5

Khutsishvili SS, Perfileva AI, Nozhkina OA

36. Oleanolic Acid Alleviates Atopic Dermatitis-like Responses In Vivo and In Vitro.

Int J Mol Sci

2021 Nov 5

Kang YM, Kim HM, Lee M

37. Decoding the Role of DVL1 in Intracranial Meningioma.

Int J Mol Sci

2021 Nov 5

Bukovac A, Dragičević K, Kafka A

38. Identification of Spiro-Fused Pyrrolo[3,4-a]pyrrolizines and Tryptanthrines as Potential Antitumor Agents: Synthesis and In Vitro Evaluation.

Int J Mol Sci

2021 Nov 5

Latypova DK, Shmakov SV, Pechkovskaya SA

39. MMP9 Differentially Regulates Proteins Involved in Actin Polymerization and Cell Migration during TGF-β-Induced EMT in the Lens.

Int J Mol Sci

2021 Nov 5

Liu ZZG, Taiyab A, West-Mays JA.

40. Effect of Central Administration of Brain-Derived Neurotrophic Factor (BDNF) on Behavior and Brain Monoamine Metabolism in New Recombinant Mouse Lines Differing by 5-HT(1A) Receptor Functionality.

Int J Mol Sci

2021 Nov 5

Bazovkina D, Naumenko V, Bazhenova E

41. Ultra-Fine Control of Silica Shell Thickness on Silver Nanoparticle-Assembled Structures.

Int J Mol Sci

2021 Nov 5

Hahm E, Jo A, Kang EJ

42. Improvement of an Effective Protocol for Directed Differentiation of Human Adipose Tissue-Derived Adult Mesenchymal Stem Cells to Corneal Endothelial Cells.

Int J Mol Sci

2021 Nov 5

Marta CM, Adrian M, Jorge FD

43. Ciprofloxacin and Levofloxacin as Potential Drugs in Genitourinary Cancer Treatment-The Effect of Dose-Response on 2D and 3D Cell Cultures.

Int J Mol Sci

2021 Nov 4

Kloskowski T, Szeliski K, Fekner Z

44. Genome-Wide Identification of ARF Gene Family Suggests a Functional Expression Pattern during Fruitlet Abscission in Prunus avium L.

Int J Mol Sci

2021 Nov 4

Hou Q, Qiu Z, Wen Z

45. The First De Novo Transcriptome Assembly and Transcriptomic Dynamics of the Mangrove Tree Rhizophora stylosa Griff. (Rhizophoraceae).

Int J Mol Sci

2021 Nov 4

Miryeganeh M, Saze H.

46. Proper Balance of Small GTPase rab10 Is Critical for PGC Migration in Zebrafish.

Int J Mol Sci

2021 Nov 4

Mo C, Li W, Jia K

47. BRAF and MEK Inhibitors Affect Dendritic-Cell Maturation and T-Cell Stimulation.

Int J Mol Sci

2021 Nov 4

Hoyer S, Eberlein V, Schuler G

48. Spike Formation Is a Turning Point Determining Wheat Root Microbiome Abundance, Structures and Functions.

Int J Mol Sci

2021 Nov 4

Usyskin-Tonne A, Hadar Y, Minz D.

Root selection of their associated microbiome composition and activities is determined by the plant's developmental stage and distance from the root. Total gene abundance, structure and functions of root-associated and rhizospheric microbiomes were studied throughout wheat growth season under field conditions. On the root surface, abundance of the well-known wheat colonizers Proteobacteria and Actinobacteria decreased and increased, respectively, during spike formation, whereas abundance of Bacteroidetes was independent of spike formation. Metagenomic analysis combined with functional co-occurrence networks revealed a significant impact of plant developmental stage on its microbiome during the transition from vegetative growth to spike formation. For example, gene functions related to biofilm and sensorial movement, antibiotic production and resistance and carbons and amino acids and their transporters. Genes associated with these functions were also in higher abundance in root vs. the rhizosphere microbiome. We propose that abundance of transporter-encoding genes related to carbon and amino acid, may mirror the availability and utilization of root exudates. Genes related to antibiotic resistance mechanisms were abundant during vegetative growth, while after spike formation, genes related to the biosynthesis of various antibiotics were enriched. This observation suggests that during root colonization and biofilm formation, bacteria cope with competitor's antibiotics, whereas in the mature biofilm stage, they invest in inhibiting new colonizers. Additionally, there is higher abundance of genes related to denitrification in rhizosphere compared to root-associated microbiome during wheat growth, possibly due to competition with the plant over nitrogen in the root vicinity. We demonstrated functional and phylogenetic division in wheat root zone microbiome in both time and space: pre- and post-spike formation, and root-associated vs. rhizospheric niches. These findings shed light on the dynamics of plant-microbe and microbe-microbe interactions in the developing root zone.

49. Cross-Tolerance and Autoimmunity as Missing Links in Abiotic and Biotic Stress Responses in Plants: A Perspective toward Secondary Metabolic Engineering.

Int J Mol Sci

2021 Nov 4

Perincherry L, Stępień Ł, Vasudevan SE.

50. Genetic Variants of the NF-κB Pathway: Unraveling the Genetic Architecture of Psoriatic Disease.

Int J Mol Sci

2021 Nov 30

Queiro R, Coto P, González-Lara L

51. Protective Effects of Glutamine and Leucine Supplementation on Sepsis-Induced Skeletal Muscle Injuries.

Int J Mol Sci

2021 Nov 30

Hou YC, Pai MH, Wu JM

52. Regulation of Cytosolic pH: The Contributions of Plant Plasma Membrane H(+)-ATPases and Multiple Transporters.

Int J Mol Sci

2021 Nov 30

Zhou JY, Hao DL, Yang GZ.

53. Skeletal Ryanodine Receptors Are Involved in Impaired Myogenic Differentiation in Duchenne Muscular Dystrophy Patients.

Int J Mol Sci

2021 Nov 30

Meyer P, Notarnicola C, Meli AC

54. Multimodal Spectroscopic Imaging of Pea Root Nodules to Assess the Nitrogen Fixation in the Presence of Biofertilizer Based on Nod-Factors.

Int J Mol Sci

2021 Nov 30

Susniak K, Krysa M, Kidaj D

55. Molecular Network for Regulation of Ovule Number in Plants.

Int J Mol Sci

2021 Nov 30

Qadir M, Wang X, Shah SRU

56. Kinetic Study on the Reactivity of Azanone (HNO) toward Cyclic C-Nucleophiles.

Int J Mol Sci

2021 Nov 30

Artelska A, Rola M, Rostkowski M

57. Reassessment of SST4 Somatostatin Receptor Expression Using SST4-eGFP Knockin Mice and the Novel Rabbit Monoclonal Anti-Human SST4 Antibody 7H49L61.

Int J Mol Sci

2021 Nov 30

Lupp A, Ehms B, Stumm R

58. Exploiting Structural Modelling Tools to Explore Host-Translocated Effector Proteins.

Int J Mol Sci

2021 Nov 30

Amoozadeh S, Johnston J, Meisrimler CN.

59. Natural Gums as Oleogelators.

Int J Mol Sci

2021 Nov 30

Banaś K, Harasym J.

60. Royal Jelly Protects against Epidermal Stress through Upregulation of the NQO1 Expression.

Int J Mol Sci

2021 Nov 30

Okumura N, Ito T, Degawa T

61. Integration of Light and Brassinosteroid Signaling during Seedling Establishment.

Int J Mol Sci

2021 Nov 30

Lin F, Cao J, Yuan J

62. Role of Defect Engineering and Surface Functionalization in the Design of Carbon Nanotube-Based Nitrogen Oxide Sensors.

Int J Mol Sci

2021 Nov 30

Valdés-Madrigal MA, Montejo-Alvaro F, Cernas-Ruiz AS

63. Fetal Immunomodulatory Environment Following Cartilage Injury-The Key to CARTILAGE Regeneration?

Int J Mol Sci

2021 Nov 30

Ribitsch I, Bileck A, Egerbacher M

64. A Review on the Role of Non-Coding RNAs in the Pathogenesis of Myasthenia Gravis.

Int J Mol Sci

2021 Nov 30

Ghafouri-Fard S, Azimi T, Hussen BM

65. The Effect of Organoselenium Compounds on Histone Deacetylase Inhibition and Their Potential for Cancer Therapy.

Int J Mol Sci

2021 Nov 30

Adimulam T, Arumugam T, Foolchand A

66. Role of Nociceptin/Orphanin FQ-NOP Receptor System in the Regulation of Stress-Related Disorders.

Int J Mol Sci

2021 Nov 30

Ubaldi M, Cannella N, Borruto AM

67. Role of ANGPTL8 in NAFLD Improvement after Bariatric Surgery in Experimental and Human Obesity.

Int J Mol Sci

2021 Nov 30

Perdomo CM, Gómez-Ambrosi J, Becerril S

68. Malondialdehyde-Acetaldehyde Modified (MAA) Proteins Differentially Effect the Inflammatory Response in Macrophage, Endothelial Cells and Animal Models of Cardiovascular Disease.

Int J Mol Sci

2021 Nov 30

Duryee MJ, Clemens DL, Opperman PJ

Chronic inflammation plays a critical role in the pathogenesis of atherosclerosis. Currently, the mechanism(s) by which inflammation contributes to this disease are not entirely understood. Inflammation is known to induce oxidative stress, which can lead to lipid peroxidation. Lipid peroxidation can result in the production of reactive by-products that can oxidatively modify macromolecules including DNA, proteins, and lipoproteins. A major reactive by-product of lipid peroxidation is malondialdehyde (MDA). MDA can subsequently break down to form acetaldehyde (AA). These two aldehydes can covalently interact with the epsilon (ε)-amino group of lysines within proteins and lipoproteins leading to the formation of extremely stable, highly immunogenic malondialdehyde/acetaldehyde adducts (MAA-adducts). The aim of this study was to investigate the inflammatory response to MAA-modified human serum albumin (HSA-MAA) and low-density lipoprotein (LDL-MAA). We found that animals injected with LDL-MAA generate antibodies specific to MAA-adducts. The level of anti-MAA antibodies were further increased in an animal model of atherosclerosis fed a Western diet. An animal model that combined both high fat diet and immunization of MAA-modified protein resulted in a dramatic increase in antibodies to MAA-adducts and vascular fat accumulation compared with controls. In vitro exposure of endothelial cells and macrophages to MAA-modified proteins resulted in increased fat accumulation as well as increased expression of adhesion molecules and pro-inflammatory cytokines. The expression of cytokines varied between the different cell lines and was unique to the individual modified proteins. The results of these studies demonstrate that different MAA-modified proteins elicit unique responses in different cell types. Additionally, the presence of MAA-modified proteins appears to modulate cellular metabolism leading to increased accumulation of triglycerides and further progression of the inflammatory response.

69. Early Development of the GABAergic System and the Associated Risks of Neonatal Anesthesia.

Int J Mol Sci

2021 Nov 30

Gascoigne DA, Serdyukova NA, Aksenov DP.

70. Prenatal Octamethylcyclotetrasiloxane Exposure Impaired Proliferation of Neuronal Progenitor, Leading to Motor, Cognition, Social and Behavioral Functions.

Int J Mol Sci

2021 Nov 30

Tran DN, Park SM, Jung EM

71. Initiation and Execution of Programmed Cell Death and Regulation of Reactive Oxygen Species in Plants.

Int J Mol Sci

2021 Nov 30

Ye C, Zheng S, Jiang D

72. Biosynthesis and Degradation of Sulfur Modifications in tRNAs.

Int J Mol Sci

2021 Nov 3

Shigi N.

73. Fibronectin 1B Gene Plays an Important Role in Loach Barbel Air-Breathing.

Int J Mol Sci

2021 Nov 3

Sun B, Huang S, Huang L

74. The Role of Endothelium in COVID-19.

Int J Mol Sci

2021 Nov 3

Ionescu M, Stoian AP, Rizzo M

75. High-Molecular-Weight Hyaluronic Acid Inhibits IL-1β-Induced Synovial Inflammation and Macrophage Polarization through the GRP78-NF-κB Signaling Pathway.

Int J Mol Sci

2021 Nov 3

Lee CH, Chiang CF, Kuo FC

76. Relevance of BRAF Subcellular Localization and Its Interaction with KRAS and KIT Mutations in Skin Melanoma.

Int J Mol Sci

2021 Nov 3

Beleaua MA, Jung I, Braicu C

Although skin melanoma (SKM) represents only one-quarter of newly diagnosed skin malignant tumors, it presents a high mortality rate. Hence, new prognostic and therapeutic tools need to be developed. This study focused on investigating the prognostic value of the subcellular expression of BRAF, KRAS, and KIT in SKM in correlation with their gene-encoding interactions. In silico analysis of the abovementioned gene interactions, along with their mRNA expression, was conducted, and the results were validated at the protein level using immunohistochemical (IHC) stains. For IHC expression, the encoded protein expressions were checked on 96 consecutive SKMs and 30 nevi. The UALCAN database showed no prognostic value for the mRNA expression level of KRAS and BRAF and demonstrated a longer survival for patients with low mRNA expression of KIT in SKMs. IHC examinations of SKMs confirmed the UALCAN data and showed that KIT expression was inversely correlated with ulceration, Breslow index, mitotic rate, and pT stage. KRAS expression was also found to be inversely correlated with ulceration and perineural invasion. When the subcellular expression of BRAF protein was recorded (nuclear vs. cytoplasmatic vs. mixed nucleus + cytoplasm), a direct correlation was emphasized between nuclear positivity and lymphovascular or perineural invasion. The independent prognostic value was demonstrated for mixed expression of the BRAF protein in SKM. BRAF cytoplasmic predominance, in association with KIT's IHC positivity, was more frequently observed in early-stage nonulcerated SKMs, which displayed a low mitotic rate and a late death event. The present study firstly verified the possible prognostic value of BRAF subcellular localization in SKMs. A low mRNA expression or IHC cytoplasmic positivity for KIT and BRAF might be used as a positive prognostic parameter of SKM. SKM's BRAF nuclear positivity needs to be evaluated in further studies as a possible indicator of perineural and lymphovascular invasion.

77. Interplay between Prokineticins and Histone Demethylase KDM6A in a Murine Model of Bortezomib-Induced Neuropathy.

Int J Mol Sci

2021 Nov 3

Rullo L, Franchi S, Amodeo G

78. Biotin Transport-Targeting Polysaccharide-Modified PAMAM G3 Dendrimer as System Delivering α-Mangostin into Cancer Cells and C. elegans Worms.

Int J Mol Sci

2021 Nov 29

Markowicz J, Uram Ł, Wołowiec S

The natural xanthone α-mangostin (αM) exhibits a wide range of pharmacological activities, including antineoplastic and anti-nematode properties, but low water solubility and poor selectivity of the drug prevent its potential clinical use. Therefore, the targeted third-generation poly(amidoamine) dendrimer (PAMAM G3) delivery system was proposed, based on hyperbranched polymer showing good solubility, high biocompatibility and low immunogenicity. A multifunctional nanocarrier was prepared by attaching αM to the surface amine groups of dendrimer via amide bond in the ratio 5 (G32B12gh5M) or 17 (G32B10gh17M) residues per one dendrimer molecule. Twelve or ten remaining amine groups were modified by conjugation with D-glucoheptono-1,4-lactone (gh) to block the amine groups, and two biotin (B) residues as targeting moieties. The biological activity of the obtained conjugates was studied in vitro on glioma U-118 MG and squamous cell carcinoma SCC-15 cancer cells compared to normal fibroblasts (BJ), and in vivo on a model organism Caenorhabditis elegans. Dendrimer vehicle G32B12gh at concentrations up to 20 µM showed no anti-proliferative effect against tested cell lines, with a feeble cytotoxicity of the highest concentration seen only with SCC-15 cells. The attachment of αM to the vehicle significantly increased cytotoxic effect of the drug, even by 4- and 25-fold for G32B12gh5M and G32B10gh17M, respectively. A stronger inhibition of cells viability and influence on other metabolic parameters (proliferation, adhesion, ATP level and Caspase-3/7 activity) was observed for G32B10gh17M than for G32B12gh5M. Both bioconjugates were internalized efficiently into the cells. Similarly, the attachment of αM to the dendrimer vehicle increased its toxicity for C. elegans. Thus, the proposed α-mangostin delivery system allowed the drug to be more effective in the dendrimer-bound as compared to free state against both cultured the cancer cells and model organism, suggesting that this treatment is promising for anticancer as well as anti-nematode chemotherapy.

79. Gastrointestinal Dopamine in Inflammatory Bowel Diseases: A Systematic Review.

Int J Mol Sci

2021 Nov 29

Kurnik-Łucka M, Pasieka P, Łączak P

80. Nutraceutical Approach to Chronic Osteoarthritis: From Molecular Research to Clinical Evidence.

Int J Mol Sci

2021 Nov 29

Colletti A, Cicero AFG.

81. Combined Blockade of TIGIT and CD39 or A2AR Enhances NK-92 Cell-Mediated Cytotoxicity in AML.

Int J Mol Sci

2021 Nov 29

Brauneck F, Seubert E, Wellbrock J

82. Optimizing Release of Nucleic Acids of African Swine Fever Virus and Influenza A Virus from FTA Cards.

Int J Mol Sci

2021 Nov 29

Elnagar A, Harder TC, Blome S

83. Applications of DNA-Functionalized Proteins.

Int J Mol Sci

2021 Nov 29

Gong Z, Tang Y, Ma N

84. Salivary Biomarkers in Patients with Sjögren's Syndrome-A Systematic Review.

Int J Mol Sci

2021 Nov 29

Jung JY, Kim JW, Kim HA

85. Comparative Transcriptome Analysis Revealed the Key Genes Regulating Ascorbic Acid Synthesis in Actinidia.

Int J Mol Sci

2021 Nov 29

Liu X, Xie X, Zhong C

86. Genomic and Metabolic Characteristics of the Pathogenicity in Pseudomonas aeruginosa.

Int J Mol Sci

2021 Nov 29

de Sousa T, Hébraud M, Dapkevicius MLNE

87. LSSmScarlet, dCyRFP2s, dCyOFP2s and CRISPRed2s, Genetically Encoded Red Fluorescent Proteins with a Large Stokes Shift.

Int J Mol Sci

2021 Nov 28

Subach OM, Vlaskina AV, Agapova YK

Genetically encoded red fluorescent proteins with a large Stokes shift (LSSRFPs) can be efficiently co-excited with common green FPs both under single- and two-photon microscopy, thus enabling dual-color imaging using a single laser. Recent progress in protein development resulted in a great variety of novel LSSRFPs; however, the selection of the right LSSRFP for a given application is hampered by the lack of a side-by-side comparison of the LSSRFPs' performance. In this study, we employed rational design and random mutagenesis to convert conventional bright RFP mScarlet into LSSRFP, called LSSmScarlet, characterized by excitation/emission maxima at 470/598 nm. In addition, we utilized the previously reported LSSRFPs mCyRFP1, CyOFP1, and mCRISPRed as templates for directed molecular evolution to develop their optimized versions, called dCyRFP2s, dCyOFP2s and CRISPRed2s. We performed a quantitative assessment of the developed LSSRFPs and their precursors in vitro on purified proteins and compared their brightness at 488 nm excitation in the mammalian cells. The monomeric LSSmScarlet protein was successfully utilized for the confocal imaging of the structural proteins in live mammalian cells and multicolor confocal imaging in conjugation with other FPs. LSSmScarlet was successfully applied for dual-color two-photon imaging in live mammalian cells. We also solved the X-ray structure of the LSSmScarlet protein at the resolution of 1.4 Å that revealed a hydrogen bond network supporting excited-state proton transfer (ESPT). Quantum mechanics/molecular mechanics molecular dynamic simulations confirmed the ESPT mechanism of a large Stokes shift. Structure-guided mutagenesis revealed the role of R198 residue in ESPT that allowed us to generate a variant with improved pH stability. Finally, we showed that LSSmScarlet protein is not appropriate for STED microscopy as a consequence of LSSRed-to-Red photoconversion with high-power 775 nm depletion light.

88. Effect of 2-Cys Peroxiredoxins Inhibition on Redox Modifications of Bull Sperm Proteins.

Int J Mol Sci

2021 Nov 28

Mostek-Majewska A, Janta A, Majewska A

89. Etifoxine Restores Mitochondrial Oxidative Phosphorylation and Improves Cognitive Recovery Following Traumatic Brain Injury.

Int J Mol Sci

2021 Nov 28

Palzur E, Edelman D, Sakas R

90. Patterns of Maternal Neutrophil Gene Expression at 30 Weeks of Gestation, but Not DNA Methylation, Distinguish Mild from Severe Preeclampsia.

Int J Mol Sci

2021 Nov 28

Walsh SW, Al Dulaimi M, Archer KJ

Neutrophils are activated and extensively infiltrate blood vessels in preeclamptic women. To identify genes that contribute to neutrophil activation and infiltration, we analyzed the transcriptomes of circulating neutrophils from normal pregnant and preeclamptic women. Neutrophils were collected at 30 weeks' gestation and RNA and DNA were isolated for RNA sequencing and 5-hydroxy-methylcytosine (5-hmC) sequencing as an index of dynamic changes in neutrophil DNA methylation. Women with normal pregnancy who went on to develop mild preeclampsia at term had the most uniquely expressed genes (697) with 325 gene ontology pathways upregulated, many related to neutrophil activation and function. Women with severe preeclampsia who delivered prematurely had few pathways up- or downregulated. Cluster analysis revealed that gene expression in women with severe preeclampsia was an inverse mirror image of gene expression in normal pregnancy, while gene expression in women who developed mild preeclampsia was remarkably different from both. DNA methylation marks, key regulators of gene expression, are removed by the action of ten-eleven translocation (TET) enzymes, which oxidize 5-methylcytosines (5mCs), resulting in locus-specific reversal of DNA methylation. DNA sequencing for 5-hmC revealed no differences among the three groups. Genome-wide DNA methylation revealed extremely low levels in circulating neutrophils suggesting they are de-methylated. Collectively, these data demonstrate that neutrophil gene expression profiles can distinguish different preeclampsia phenotypes, and in the case of mild preeclampsia, alterations in gene expression occur well before clinical symptoms emerge. These findings serve as a foundation for further evaluation of neutrophil transcriptomes as biomarkers of preeclampsia phenotypes. Changes in DNA methylation in circulating neutrophils do not appear to mediate differential patterns of gene expression in either mild or severe preeclampsia.

91. Revisiting Hepatic Artery Infusion Chemotherapy in the Treatment of Advanced Hepatocellular Carcinoma.

Int J Mol Sci

2021 Nov 28

Chen CT, Liu TH, Shao YY

92. Cyclic Nucleotide (cNMP) Analogues: Past, Present and Future.

Int J Mol Sci

2021 Nov 28

Maronde E.

93. Evidence for the Involvement of Pleckstrin Homology Domain-Containing Proteins in the Transport of Enterocin DD14 (EntDD14); a Leaderless Two-Peptide Bacteriocin.

Int J Mol Sci

2021 Nov 28

Pérez-Ramos A, Ladjouzi R, Benachour A

94. Study of the Spatio-Chemical Heterogeneity of Tannin-Furanic Foams: From 1D FTIR Spectroscopy to 3D FTIR Micro-Computed Tomography.

Int J Mol Sci

2021 Nov 28

Cefarin N, Bedolla DE, Surowka A

95. Deepening the Knowledge of ROS1 Rearrangements in Non-Small Cell Lung Cancer: Diagnosis, Treatment, Resistance and Concomitant Alterations.

Int J Mol Sci

2021 Nov 28

Guaitoli G, Bertolini F, Bettelli S

96. Structural Modelling of KCNQ1 and KCNH2 Double Mutant Proteins, Identified in Two Severe Long QT Syndrome Cases, Reveals New Insights into Cardiac Channelopathies.

Int J Mol Sci

2021 Nov 28

Agudelo WA, Gil-Quiñones SR, Fonseca A

97. Data-Independent Acquisition-Based Proteome and Phosphoproteome Profiling Reveals Early Protein Phosphorylation and Dephosphorylation Events in Arabidopsis Seedlings upon Cold Exposure.

Int J Mol Sci

2021 Nov 27

Tan J, Zhou Z, Feng H

98. Histone 3 Lysine 27 Trimethylation Signature in Breast Cancer.

Int J Mol Sci

2021 Nov 27

Borkiewicz L.

99. Physiological and Dual Transcriptional Analysis of Microalga Graesiella emersonii-Amoeboaphelidium protococcarum Pathosystem Uncovers Conserved Defense Response and Robust Pathogenicity.

Int J Mol Sci

2021 Nov 27

Ding Y, Wang Z, Wang Y

100. Sensitive Immunofluorescent Detection of the PRAME Antigen Using a Practical Antibody Conjugation Approach.

Int J Mol Sci

2021 Nov 27

Sapozhnikova KA, Misyurin VA, Ryazantsev DY

101. The Complexity of the Ovine and Caprine Keratin-Associated Protein Genes.

Int J Mol Sci

2021 Nov 27

Zhou H, Gong H, Wang J

102. Macrophages Modulate the Function of MSC- and iPSC-Derived Fibroblasts in the Presence of Polyethylene Particles.

Int J Mol Sci

2021 Nov 27

Gao Q, Li Z, Rhee C

103. Evaluation of Deep Neural Network ProSPr for Accurate Protein Distance Predictions on CASP14 Targets.

Int J Mol Sci

2021 Nov 27

Stern J, Hedelius B, Fisher O

104. VDACs Post-Translational Modifications Discovery by Mass Spectrometry: Impact on Their Hub Function.

Int J Mol Sci

2021 Nov 27

Pittalà MGG, Conti Nibali S, Reina S

105. The Mechanisms Underlying the Protective Action of Selenium Nanoparticles against Ischemia/Reoxygenation Are Mediated by the Activation of the Ca(2+) Signaling System of Astrocytes and Reactive Astrogliosis.

Int J Mol Sci

2021 Nov 26

Varlamova EG, Turovsky EA, Babenko VA

In recent years, much attention has been paid to the study of the therapeutic effect of the microelement selenium, its compounds, especially selenium nanoparticles, with a large number of works devoted to their anticancer effects. Studies proving the neuroprotective properties of selenium nanoparticles in various neurodegenerative diseases began to appear only in the last 5 years. Nevertheless, the mechanisms of the neuroprotective action of selenium nanoparticles under conditions of ischemia and reoxygenation remain unexplored, especially for intracellular Ca2+ signaling and neuroglial interactions. This work is devoted to the study of the cytoprotective mechanisms of selenium nanoparticles in the neuroglial networks of the cerebral cortex under conditions of ischemia/reoxygenation. It was shown for the first time that selenium nanoparticles dose-dependently induce the generation of Ca2+ signals selectively in astrocytes obtained from different parts of the brain. The generation of these Ca2+ signals by astrocytes occurs through the release of Ca2+ ions from the endoplasmic reticulum through the IP3 receptor upon activation of the phosphoinositide signaling pathway. An increase in the concentration of cytosolic Ca2+ in astrocytes leads to the opening of connexin Cx43 hemichannels and the release of ATP and lactate into the extracellular medium, which trigger paracrine activation of the astrocytic network through purinergic receptors. Incubation of cerebral cortex cells with selenium nanoparticles suppresses ischemia-induced increase in cytosolic Ca2+ and necrotic cell death. Activation of A2 reactive astrocytes exclusively after ischemia/reoxygenation, a decrease in the expression level of a number of proapoptotic and proinflammatory genes, an increase in lactate release by astrocytes, and suppression of the hyperexcitation of neuronal networks formed the basis of the cytoprotective effect of selenium nanoparticles in our studies.

106. β-Cell Pathophysiology: A Review of Advanced Optical Microscopy Applications.

Int J Mol Sci

2021 Nov 26

Ferri G, Pesce L, Tesi M

107. Volume-Independent Sodium Toxicity in Peritoneal Dialysis: New Insights from Bench to Bed.

Int J Mol Sci

2021 Nov 26

Borrelli S, De Nicola L, De Gregorio I

108. Differential Role of p53 in Oligodendrocyte Survival in Response to Various Stresses: Experimental Autoimmune Encephalomyelitis, Cuprizone Intoxication or White Matter Stroke.

Int J Mol Sci

2021 Nov 26

Luo F, Zhang Z, Luo Y.

109. Erythrocytes Prevent Degradation of Carnosine by Human Serum Carnosinase.

Int J Mol Sci

2021 Nov 26

Oppermann H, Elsel S, Birkemeyer C

110. Transmission Electron Microscopy as a Powerful Tool to Investigate the Interaction of Nanoparticles with Subcellular Structures.

Int J Mol Sci

2021 Nov 26

Malatesta M.

111. Lessons Learned from the Studies of Roots Shaded from Direct Root Illumination.

Int J Mol Sci

2021 Nov 26

Lacek J, García-González J, Weckwerth W

112. Cytotoxic Aβ Protofilaments Are Generated in the Process of Aβ Fibril Disaggregation.

Int J Mol Sci

2021 Nov 26

Kaku T, Tsukakoshi K, Ikebukuro K.

113. Development of Minicircle Vectors Encoding COL7A1 Gene with Human Promoters for Non-Viral Gene Therapy for Recessive Dystrophic Epidermolysis Bullosa.

Int J Mol Sci

2021 Nov 26

Wang X, Alshehri F, Manzanares D

114. Modified Low-Temperature Extraction Method for Isolation of Bletilla striata Polysaccharide as Antioxidant for the Prevention of Alzheimer's Disease.

Int J Mol Sci

2021 Nov 25

Lin YW, Fang CH, Liang YJ

115. Transcription Regulators and Membraneless Organelles Challenges to Investigate Them.

Int J Mol Sci

2021 Nov 25

Sołtys K, Ożyhar A.

116. Emerging View on the Molecular Functions of Sec62 and Sec63 in Protein Translocation.

Int J Mol Sci

2021 Nov 25

Jung SJ, Kim H.

117. Prognostic Value of BUB1 for Predicting Non-Muscle-Invasive Bladder Cancer Progression.

Int J Mol Sci

2021 Nov 25

Piao XM, You C, Byun YJ

118. Dexmedetomidine Promotes Lipopolysaccharide-Induced Differentiation of Cardiac Fibroblasts and Collagen I/III Synthesis through α(2A) Adrenoreceptor-Mediated Activation of the PKC-p38-Smad2/3 Signaling Pathway in Mice.

Int J Mol Sci

2021 Nov 25

Liao J, Li K, Su X

119. The Role of Toll-like Receptors (TLRs) Mediated Inflammation in Pancreatic Cancer Pathophysiology.

Int J Mol Sci

2021 Nov 25

Orlacchio A, Mazzone P.

120. CCL4 Stimulates Cell Migration in Human Osteosarcoma via the mir-3927-3p/Integrin αvβ3 Axis.

Int J Mol Sci

2021 Nov 25

Tsai HC, Lai YY, Hsu HC

121. Osteoporosis: From Molecular Mechanisms to Therapies 3.0.

Int J Mol Sci

2021 Nov 25

Tang CH.

122. Correction: Shao et al. AKT Axis, miR-21, and RECK Play Pivotal Roles in Dihydroartemisinin Killing Malignant Glioma Cells. Int. J. Mol. Sci. 2017, 18, 350.

Int J Mol Sci

2021 Nov 24

Shao YY, Zhang TL, Wu LX

123. The Pellicle-Another Strategy of the Root Apex Protection against Mechanical Stress?

Int J Mol Sci

2021 Nov 24

Potocka I, Szymanowska-Pułka J.

124. Sulfur-Induced Resistance against Pseudomonas syringae pv. actinidiae via Triggering Salicylic Acid Signaling Pathway in Kiwifruit.

Int J Mol Sci

2021 Nov 24

Zhang Z, Long Y, Yin X

125. Placental Endocrine Activity: Adaptation and Disruption of Maternal Glucose Metabolism in Pregnancy and the Influence of Fetal Sex.

Int J Mol Sci

2021 Nov 24

Stern C, Schwarz S, Moser G

126. Fine Breakpoint Mapping by Genome Sequencing Reveals the First Large X Inversion Disrupting the NHS Gene in a Patient with Syndromic Cataracts.

Int J Mol Sci

2021 Nov 24

Damián A, Ionescu RO, Rodríguez de Alba M

127. HbSnRK2.6 Functions in ABA-Regulated Cold Stress Response by Promoting HbICE2 Transcriptional Activity in Hevea brasiliensis.

Int J Mol Sci

2021 Nov 24

Wang X, Liu WC, Zeng XW

128. An In Vitro Evaluation of the Biological and Osteogenic Properties of Magnesium-Doped Bioactive Glasses for Application in Bone Tissue Engineering.

Int J Mol Sci

2021 Nov 24

Hohenbild F, Arango Ospina M, Schmitz SI

129. Rare Glutamic Acid Methyl Ester Peptaibols from Sepedonium ampullosporum Damon KSH 534 Exhibit Promising Antifungal and Anticancer Activity.

Int J Mol Sci

2021 Nov 24

Lam YTH, Ricardo MG, Rennert R

130. Effects of the Combined Treatment with a G-Quadruplex-Stabilizing Ligand and Photon Beams on Glioblastoma Stem-like Cells: A Magnetic Resonance Study.

Int J Mol Sci

2021 Nov 24

Palma A, Grande S, Luciani AM

131. GRPEL2 Knockdown Exerts Redox Regulation in Glioblastoma.

Int J Mol Sci

2021 Nov 24

Tang CT, Li YF, Chou CH

132. Porphyromonas gingivalis Components/Secretions Synergistically Enhance Pneumonia Caused by Streptococcus pneumoniae in Mice.

Int J Mol Sci

2021 Nov 24

Okabe T, Kamiya Y, Kikuchi T

133. Phytosterols in Seaweeds: An Overview on Biosynthesis to Biomedical Applications.

Int J Mol Sci

2021 Nov 24

Sohn SI, Rathinapriya P, Balaji S

134. Impact of Hydrogen Sulfide on Mitochondrial and Bacterial Bioenergetics.

Int J Mol Sci

2021 Nov 24

Borisov VB, Forte E.

135. Comparing C2=O and C2=S Barbiturates: Different Hydrogen-Bonding Patterns of Thiobarbiturates in Solution and the Solid State.

Int J Mol Sci

2021 Nov 24

Li C, Hilgeroth P, Hasan N

136. Adamantane-Substituted Purines and Their β-Cyclodextrin Complexes: Synthesis and Biological Activity.

Int J Mol Sci

2021 Nov 24

Rouchal M, Rudolfová J, Kryštof V

137. Progressive Aggregation of 16 kDa Gamma-Zein during Seed Maturation in Transgenic Arabidopsis thaliana.

Int J Mol Sci

2021 Nov 24

Arcalis E, Mainieri D, Vitale A

138. C57BL/6 Background Attenuates mHTT Toxicity in the Striatum of YAC128 Mice.

Int J Mol Sci

2021 Nov 23

Back MK, Kurzawa J, Ruggieri S

139. The Significance of Biomechanics and Scaffold Structure for Bladder Tissue Engineering.

Int J Mol Sci

2021 Nov 23

Hanczar M, Moazen M, Day R.

140. Paradigmatic De Novo GRIN1 Variants Recapitulate Pathophysiological Mechanisms Underlying GRIN1-Related Disorder Clinical Spectrum.

Int J Mol Sci

2021 Nov 23

Santos-Gómez A, Miguez-Cabello F, Juliá-Palacios N

141. CAF Proteins Help SOT1 Regulate the Stability of Chloroplast ndhA Transcripts.

Int J Mol Sci

2021 Nov 23

Li X, Luo W, Zhou W

142. Natural Bioactive Molecules: An Alternative Approach to the Treatment and Control of COVID-19.

Int J Mol Sci

2021 Nov 23

Islam F, Bibi S, Meem AFK

143. Heterogenous Clinical Landscape in a Consanguineous Malonic Aciduria Family.

Int J Mol Sci

2021 Nov 23

Snanoudj S, Torre S, Sudrié-Arnaud B

144. A Talk between Flavonoids and Hormones to Reorient the Growth of Gymnosperms.

Int J Mol Sci

2021 Nov 23

Morales-Quintana L, Ramos P.

145. Unveiling the Complexity of Red Clover (Trifolium pratense L.) Transcriptome and Transcriptional Regulation of Isoflavonoid Biosynthesis Using Integrated Long- and Short-Read RNAseq.

Int J Mol Sci

2021 Nov 23

Shi K, Liu X, Pan X

Red clover (Trifolium pratense L.) is used as forage and contains a high level of isoflavonoids. Although isoflavonoids in red clover were discovered a long time ago, the transcriptional regulation of isoflavonoid biosynthesis is virtually unknown because of the lack of accurate and comprehensive characterization of the transcriptome. Here, we used a combination of long-read (PacBio Iso-Seq) and short-read (Illumina) RNAseq sequencing to develop a more comprehensive full-length transcriptome in four tissues (root, stem, leaf, and flower) and to identify transcription factors possibly involved in isoflavonoid biosynthesis in red clover. Overall, we obtained 50,922 isoforms, including 19,860 known genes and 2817 novel isoforms based on the annotation of RefGen Tp_v2.0. We also found 1843 long non-coding RNAs, 1625 fusion genes, and 34,612 alternatively spliced events, with some transcript isoforms validated experimentally. A total of 16,734 differentially expressed genes were identified in the four tissues, including 43 isoflavonoid-biosynthesis-related genes, such as stem-specific expressed TpPAL, TpC4H, and Tp4CL and root-specific expressed TpCHS, TpCHI1, and TpIFS. Further, weighted gene co-expression network analysis and a targeted compound assay were combined to investigate the association between the isoflavonoid content and the transcription factors expression in the four tissues. Twelve transcription factors were identified as key genes for isoflavonoid biosynthesis. Among these transcription factors, the overexpression of TpMYB30 or TpRSM1-2 significantly increased the isoflavonoid content in tobacco. In particular, the glycitin was increased by 50-100 times in the plants overexpressing TpRSM1-2, in comparison to that in the WT plants. Our study provides a comprehensive and accurate annotation of the red clover transcriptome and candidate genes to improve isoflavonoid biosynthesis and accelerate research into molecular breeding in red clover or other crops.

146. Mast Cells and Basophils in the Defense against Ectoparasites: Efficient Degradation of Parasite Anticoagulants by the Connective Tissue Mast Cell Chymases.

Int J Mol Sci

2021 Nov 23

Fu Z, Akula S, Olsson AK

147. Enhanced Platelet-Rich Plasma (ePRP) Stimulates Wound Healing through Effects on Metabolic Reprogramming in Fibroblasts.

Int J Mol Sci

2021 Nov 23

Weng HP, Cheng YY, Lee HL

As a source of growth factors for expediting wound healing and tissue regeneration, plasma-rich plasma (PRP) has been extensively applied in diverse fields including orthopaedics, ophthalmology, oral and maxillofacial surgery, dentistry, and gynaecology. However, the function of PRP in metabolic regulations remains enigmatic. A standardized method was devised herein to enrich growth factors and to lyophilize it as enhanced PRP (ePRP) powder, which could become ubiquitously available without mechanical centrifugation in clinical practice. To identify metabolic reprogramming in human dermal fibroblasts under ePRP treatment, putative metabolic targets were identified by transcriptome profiling and validated for their metabolic effects and mechanism. ePRP does not only promote wound healing but re-aligns energy metabolism by shifting to glycolysis through stimulation of glycolytic enzyme activity in fibroblasts. On the contrary, oxygen consumption rates and several mitochondrial respiration activities were attenuated in ePRP-treated fibroblasts. Furthermore, ePRP treatment drives the mitochondrial resetting by hindering the mitochondrial biogenesis-related genes and results in a dampened mitochondrial mass. Antioxidant production was further increased by ePRP treatment to prevent reactive oxygen species formation. Besides, ePRP also halts the senescence progression of fibroblasts by activating SIRT1 expression. Importantly, the glycolytic inhibitor 2-DG can completely reverse the ePRP-enhanced wound healing capacity, whereas the mitochondrial inhibitor oligomycin cannot. This is the first study to utilize PRP for comprehensively investigating its effects on the metabolic reprogramming of fibroblasts. These findings indicate that PRP's primary metabolic regulation is to promote metabolic reprogramming toward glycolytic energy metabolism in fibroblasts, preserving redox equilibrium and allowing anabolic pathways necessary for the healing and anti-ageing process.

148. Plant Dehydrins: Expression, Regulatory Networks, and Protective Roles in Plants Challenged by Abiotic Stress.

Int J Mol Sci

2021 Nov 23

Sun Z, Li S, Chen W

149. Disulfide Dimerization of Neuronal Calcium Sensor-1: Implications for Zinc and Redox Signaling.

Int J Mol Sci

2021 Nov 22

Baksheeva VE, Baldin AV, Zalevsky AO

Neuronal calcium sensor-1 (NCS-1) is a four-EF-hand ubiquitous signaling protein modulating neuronal function and survival, which participates in neurodegeneration and carcinogenesis. NCS-1 recognizes specific sites on cellular membranes and regulates numerous targets, including G-protein coupled receptors and their kinases (GRKs). Here, with the use of cellular models and various biophysical and computational techniques, we demonstrate that NCS-1 is a redox-sensitive protein, which responds to oxidizing conditions by the formation of disulfide dimer (dNCS-1), involving its single, highly conservative cysteine C38. The dimer content is unaffected by the elevation of intracellular calcium levels but increases to 10-30% at high free zinc concentrations (characteristic of oxidative stress), which is accompanied by accumulation of the protein in punctual clusters in the perinuclear area. The formation of dNCS-1 represents a specific Zn2+-promoted process, requiring proper folding of the protein and occurring at redox potential values approaching apoptotic levels. The dimer binds Ca2+ only in one EF-hand per monomer, thereby representing a unique state, with decreased α-helicity and thermal stability, increased surface hydrophobicity, and markedly improved inhibitory activity against GRK1 due to 20-fold higher affinity towards the enzyme. Furthermore, dNCS-1 can coordinate zinc and, according to molecular modeling, has an asymmetrical structure and increased conformational flexibility of the subunits, which may underlie their enhanced target-binding properties. In HEK293 cells, dNCS-1 can be reduced by the thioredoxin system, otherwise accumulating as protein aggregates, which are degraded by the proteasome. Interestingly, NCS-1 silencing diminishes the susceptibility of Y79 cancer cells to oxidative stress-induced apoptosis, suggesting that NCS-1 may mediate redox-regulated pathways governing cell death/survival in response to oxidative conditions.

150. PSD-95: An Effective Target for Stroke Therapy Using Neuroprotective Peptides.

Int J Mol Sci

2021 Nov 22

Ugalde-Triviño L, Díaz-Guerra M.

151. GM3 Ganglioside Linked to Neurofibrillary Pathology in a Transgenic Rat Model for Tauopathy.

Int J Mol Sci

2021 Nov 22

Olešová D, Majerová P, Hájek R

152. Takinib Inhibits Inflammation in Human Rheumatoid Arthritis Synovial Fibroblasts by Targeting the Janus Kinase-Signal Transducer and Activator of Transcription 3 (JAK/STAT3) Pathway.

Int J Mol Sci

2021 Nov 22

Panipinto PM, Singh AK, Shaikh FS

153. Manipulating Estrogenic/Anti-Estrogenic Activity of Triphenylethylenes towards Development of Novel Anti-Neoplastic SERMs.

Int J Mol Sci

2021 Nov 22

Elnakib HE, Ramsis MM, Albably NO

154. Targeting Mitochondria by Plant Secondary Metabolites: A Promising Strategy in Combating Parkinson's Disease.

Int J Mol Sci

2021 Nov 22

Fakhri S, Abdian S, Zarneshan SN

155. Anterior Cruciate Ligament Reconstruction: Is Biological Augmentation Beneficial?

Int J Mol Sci

2021 Nov 22

Rodríguez-Merchán EC.

156. Possible Treatment of Myocardial Infarct Based on Tissue Engineering Using a Cellularized Solid Collagen Scaffold Functionalized with Arg-Glyc-Asp (RGD) Peptide.

Int J Mol Sci

2021 Nov 22

Schussler O, Falcoz PE, Chachques JC

157. Analysis of Cryopreservation Protocols and Their Harmful Effects on the Endothelial Integrity of Human Corneas.

Int J Mol Sci

2021 Nov 22

Rodríguez-Fernández S, Álvarez-Portela M, Rendal-Vázquez E

158. CD40 Pathway and IL-2 Expression Mediate the Differential Outcome of Colorectal Cancer Patients with Different CSF1R c.1085 Genotypes.

Int J Mol Sci

2021 Nov 22

Yeh YM, Lin PC, Su WC

159. The Regeneration of Large-Sized and Vascularized Adipose Tissue Using a Tailored Elastic Scaffold and dECM Hydrogels.

Int J Mol Sci

2021 Nov 22

Kim SH, Kim D, Cha M

160. Comparative Genome Analysis of Genes Regulating Compound Inflorescences in Tomato.

Int J Mol Sci

2021 Nov 21

Yang Y, Yang H, Tan Y

161. Priming, Triggering, Adaptation and Senescence (PTAS): A Hypothesis for a Common Damage Mechanism of Steatohepatitis.

Int J Mol Sci

2021 Nov 21

Abuja PM, Zatloukal K, Denk H.

162. New Investigations with Lupane Type A-Ring Azepane Triterpenoids for Antimycobacterial Drug Candidate Design.

Int J Mol Sci

2021 Nov 21

Kazakova O, Racoviceanu R, Petrova A

163. Fibrinogen and Antifibrinolytic Proteins: Interactions and Future Therapeutics.

Int J Mol Sci

2021 Nov 21

Pechlivani N, Kearney KJ, Ajjan RA.

164. Aquaporin-1 Facilitates Transmesothelial Water Permeability: In Vitro and Ex Vivo Evidence and Possible Implications in Peritoneal Dialysis.

Int J Mol Sci

2021 Nov 21

Piccapane F, Gerbino A, Carmosino M

165. Adenophora triphylla var. japonica Inhibits Candida Biofilm Formation, Increases Susceptibility to Antifungal Agents and Reduces Infection.

Int J Mol Sci

2021 Nov 21

Kim D, Kim KY.

166. New Insight into Molecular and Hormonal Connection in Andrology.

Int J Mol Sci

2021 Nov 2

Francomano D, Sanguigni V, Capogrosso P

167. Contribution of Dysregulated DNA Methylation to Autoimmunity.

Int J Mol Sci

2021 Nov 2

Funes SC, Fernández-Fierro A, Rebolledo-Zelada D

168. The Protective Role of Prolyl Oligopeptidase (POP) Inhibition in Kidney Injury Induced by Renal Ischemia-Reperfusion.

Int J Mol Sci

2021 Nov 2

Casili G, Ardizzone A, Basilotta R

169. Computationally Reconstructed Interactome of Bradyrhizobium diazoefficiens USDA110 Reveals Novel Functional Modules and Protein Hubs for Symbiotic Nitrogen Fixation.

Int J Mol Sci

2021 Nov 2

Ma JX, Yang Y, Li G

170. Association between Equol Production Status and Nonalcoholic Steatohepatitis.

Int J Mol Sci

2021 Nov 2

Akahane T, Kaya D, Noguchi R

171. A Novel Spo11 Homologue Functions as a Positive Regulator in Cyst Differentiation in Giardia lamblia.

Int J Mol Sci

2021 Nov 2

Chen YC, Tung SY, Huang CW

172. Transcriptome-Wide Gene Expression Plasticity in Stipa grandis in Response to Grazing Intensity Differences.

Int J Mol Sci

2021 Nov 2

Dang Z, Jia Y, Tian Y

173. Melatonin-Mediated Colonic Microbiota Metabolite Butyrate Prevents Acute Sleep Deprivation-Induced Colitis in Mice.

Int J Mol Sci

2021 Nov 2

Gao T, Wang Z, Dong Y

Radical cure colitis is a severe public health threat worldwide. Our previous studies have confirmed that melatonin can effectively improve gut microbiota disorder and mucosal injury caused by sleep deprivation (SD). The present study further explored the mechanism whereby exogenous melatonin prevented SD-induced colitis. 16S rRNA high-throughput sequencing and metabolomics analysis were used to explore the correlation between SD-induced colitis and intestinal microbiota and metabolite composition in mice. Fecal microbiota transplantation (FMT) and melatonin or butyrate supplementation tests verified the core role of gut microbiota in melatonin-alleviating SD-induced colitis. Further, in vitro tests studied the modulatory mechanism of metabolite butyrate. The results demonstrated that SD leads to reductions in plasma melatonin levels and colonic Card9 expression and consequent occurrence of colitis and gut microbiota disorder, especially the downregulation of Faecalibacterium and butyrate levels. The FMT from SD-mice to normal mice could restore SD-like colitis, while butyrate supplementation to SD-mice inhibited the occurrence of colitis, but with no change in the plasma melatonin level in both treatments. However, melatonin supplementation reversed all inductions in SD-mice. In intestinal epithelial cells, the inflammatory ameliorative effect of butyrate was blocked with pretreatments of HDAC3 agonist and HIF-1α antagonist but was mimicked by GSK-3β and p-P65 antagonists. Therefore, the administration of MLT may be a better therapy for SD-induced colitis relative to butyrate. A feasible mechanism would involve that melatonin up-regulated the Faecalibacterium population and production of its metabolite butyrate and MCT1 expression and inhibited HDAC3 in the colon, which would allow p-GSK-3β/β-catenin/HIF-1α activation and NF-κB/NLRP3 suppression to up-regulate Card9 expression and suppress inflammation response.

174. Recombinase Polymerase Amplification Assay with and without Nuclease-Dependent-Labeled Oligonucleotide Probe.

Int J Mol Sci

2021 Nov 2

Ivanov AV, Safenkova IV, Zherdev AV

175. Tofacitinib Ameliorates Retinal Vascular Leakage in a Murine Model of Diabetic Retinopathy with Type 2 Diabetes.

Int J Mol Sci

2021 Nov 2

Byrne EM, Llorián-Salvador M, Lyons TJ

176. Genome-Wide Identification and Characterization of Soybean GmLOR Gene Family and Expression Analysis in Response to Abiotic Stresses.

Int J Mol Sci

2021 Nov 19

Fang Y, Cao D, Yang H

177. Astragaloside IV Attenuates Ocular Hypertension in a Mouse Model of TGFβ2 Induced Primary Open Angle Glaucoma.

Int J Mol Sci

2021 Nov 19

Kasetti RB, Maddineni P, Kodati B

178. Anti-Müllerian Hormone in Pathogenesis, Diagnostic and Treatment of PCOS.

Int J Mol Sci

2021 Nov 19

Rudnicka E, Kunicki M, Calik-Ksepka A

179. Therapeutic Advances in Gut Microbiome Modulation in Patients with Inflammatory Bowel Disease from Pediatrics to Adulthood.

Int J Mol Sci

2021 Nov 19

Eindor-Abarbanel A, Healey GR, Jacobson K.

180. Neuroprotective Effect of Statins in a Rat Model of Chronic Ocular Hypertension.

Int J Mol Sci

2021 Nov 19

Kim ML, Sung KR, Kwon J

181. On the Edge of Dispensability, the Chloroplast ndh Genes.

Int J Mol Sci

2021 Nov 19

Sabater B.

182. PAHs and PCBs Affect Functionally Intercorrelated Genes in the Sea Urchin Paracentrotus lividus Embryos.

Int J Mol Sci

2021 Nov 19

Albarano L, Zupo V, Guida M

183. Cell Behavior of Non-Small Cell Lung Cancer Is at EGFR and MicroRNAs Hands.

Int J Mol Sci

2021 Nov 19

Hassanein SS, Ibrahim SA, Abdel-Mawgood AL.

184. Melatonin and Pathological Cell Interactions: Mitochondrial Glucose Processing in Cancer Cells.

Int J Mol Sci

2021 Nov 19

Reiter RJ, Sharma R, Rosales-Corral S

185. The Roles of the Colon Cancer Associated Transcript 2 (CCAT2) Long Non-Coding RNA in Cancer: A Comprehensive Characterization of the Tumorigenic and Molecular Functions.

Int J Mol Sci

2021 Nov 19

Pirlog R, Drula R, Nutu A

186. Statins and Bempedoic Acid: Different Actions of Cholesterol Inhibitors on Macrophage Activation.

Int J Mol Sci

2021 Nov 19

Linnenberger R, Hoppstädter J, Wrublewsky S

187. Controlled Transdermal Iontophoresis of Insulin from Water-Soluble Polypyrrole Nanoparticles: An In Vitro Study.

Int J Mol Sci

2021 Nov 19

Tari K, Khamoushian S, Madrakian T

188. The Rpd3-Complex Regulates Expression of Multiple Cell Surface Recycling Factors in Yeast.

Int J Mol Sci

2021 Nov 19

Amoiradaki K, Bunting KR, Paine KM

189. The Role of NLRP3 Inflammasome in Lupus Nephritis.

Int J Mol Sci

2021 Nov 19

Oliveira CB, Lima CAD, Vajgel G

190. Aquatic Toxicity Effects and Risk Assessment of 'Form Specific' Product-Released Engineered Nanomaterials.

Int J Mol Sci

2021 Nov 18

Lehutso RF, Wesley-Smith J, Thwala M.

191. Osteopontin Deficiency Ameliorates Prostatic Fibrosis and Inflammation.

Int J Mol Sci

2021 Nov 18

Popovics P, Jain A, Skalitzky KO

192. Targeting PI3K/Akt/mTOR Pathway by Different Flavonoids: A Cancer Chemopreventive Approach.

Int J Mol Sci

2021 Nov 18

Zughaibi TA, Suhail M, Tarique M

193. Dexamethasone Suppresses Palatal Cell Proliferation through miR-130a-3p.

Int J Mol Sci

2021 Nov 18

Yoshioka H, Jun G, Suzuki A

194. Characterization and Genome Study of Novel Lytic Bacteriophages against Prevailing Saprophytic Bacterial Microflora of Minimally Processed Plant-Based Food Products.

Int J Mol Sci

2021 Nov 18

Wójcicki M, Średnicka P, Błażejak S

The food industry is still searching for novel solutions to effectively ensure the microbiological safety of food, especially fresh and minimally processed food products. Nowadays, the use of bacteriophages as potential biological control agents in microbiological food safety and preservation is a promising strategy. The aim of the study was the isolation and comprehensive characterization of novel bacteriophages with lytic activity against saprophytic bacterial microflora of minimally processed plant-based food products, such as mixed leaf salads. From 43 phages isolated from municipal sewage, four phages, namely Enterobacter phage KKP 3263, Citrobacter phage KKP 3664, Enterobacter phage KKP 3262, and Serratia phage KKP 3264 have lytic activity against Enterobacter ludwigii KKP 3083, Citrobacter freundii KKP 3655, Enterobacter cloacae KKP 3082, and Serratia fonticola KKP 3084 bacterial strains, respectively. Transmission electron microscopy (TEM) and whole-genome sequencing (WGS) identified Enterobacter phage KKP 3263 as an Autographiviridae, and Citrobacter phage KKP 3664, Enterobacter phage KKP 3262, and Serratia phage KKP 3264 as members of the Myoviridae family. Genome sequencing revealed that these phages have linear double-stranded DNA (dsDNA) with sizes of 39,418 bp (KKP 3263), 61,608 bp (KKP 3664), 84,075 bp (KKP 3262), and 148,182 bp (KKP 3264). No antibiotic resistance genes, virulence factors, integrase, recombinase, or repressors, which are the main markers of lysogenic viruses, were annotated in phage genomes. Serratia phage KKP 3264 showed the greatest growth inhibition of Serratia fonticola KKP 3084 strain. The use of MOI 1.0 caused an almost 5-fold decrease in the value of the specific growth rate coefficient. The phages retained their lytic activity in a wide range of temperatures (from -20 °C to 50 °C) and active acidity values (pH from 4 to 11). All phages retained at least 70% of lytic activity at 60 °C. At 80 °C, no lytic activity against tested bacterial strains was observed. Serratia phage KKP 3264 was the most resistant to chemical factors, by maintaining high lytic activity across a broader range of pH from 3 to 11. The results indicated that these phages could be a potential biological control agent against saprophytic bacterial microflora of minimally processed plant-based food products.

195. Calciprotein Particles Link Disturbed Mineral Homeostasis with Cardiovascular Disease by Causing Endothelial Dysfunction and Vascular Inflammation.

Int J Mol Sci

2021 Nov 18

Shishkova DK, Velikanova EA, Bogdanov LA

196. Keratins as an Inflammation Trigger Point in Epidermolysis Bullosa Simplex.

Int J Mol Sci

2021 Nov 18

Evtushenko NA, Beilin AK, Kosykh AV

197. Increased Frequency of Copy Number Variations Revealed by Array Comparative Genomic Hybridization in the Offspring of Male Mice Exposed to Low Dose-Rate Ionizing Radiation.

Int J Mol Sci

2021 Nov 18

Ogura K, Ayabe Y, Harada C

198. Aryl Hydrocarbon Receptor (AhR) Activation by 2,3,7,8-Tetrachlorodibenzo-p-Dioxin (TCDD) Dose-Dependently Shifts the Gut Microbiome Consistent with the Progression of Non-Alcoholic Fatty Liver Disease.

Int J Mol Sci

2021 Nov 18

Fling RR, Zacharewski TR.

199. A Systematic Approach to the Development of Cilostazol Nanosuspension by Liquid Antisolvent Precipitation (LASP) and Its Combination with Ultrasound.

Int J Mol Sci

2021 Nov 17

Jakubowska E, Milanowski B, Lulek J.

200. Clinical Translation of Combined MAPK and Autophagy Inhibition in RAS Mutant Cancer.

Int J Mol Sci

2021 Nov 17

Lee JJ, Jain V, Amaravadi RK.

201. Comparing the Osteogenic Potential and Bone Regeneration Capacities of Dedifferentiated Fat Cells and Adipose-Derived Stem Cells In Vitro and In Vivo: Application of DFAT Cells Isolated by a Mesh Method.

Int J Mol Sci

2021 Nov 17

Takabatake K, Matsubara M, Yamachika E

202. Aesculetin Accelerates Osteoblast Differentiation and Matrix-Vesicle-Mediated Mineralization.

Int J Mol Sci

2021 Nov 17

Na W, Kang MK, Park SH

The imbalance between bone resorption and bone formation in favor of resorption results in bone loss and deterioration of bone architecture. Osteoblast differentiation is a sequential event accompanying biogenesis of matrix vesicles and mineralization of collagen matrix with hydroxyapatite crystals. Considerable efforts have been made in developing naturally-occurring plant compounds, preventing bone pathologies, or enhancing bone regeneration. Coumarin aesculetin inhibits osteoporosis through hampering the ruffled border formation of mature osteoclasts. However, little is known regarding the effects of aesculetin on the impairment of matrix vesicle biogenesis. MC3T3-E1 cells were cultured in differentiation media with 1-10 μM aesculetin for up to 21 days. Aesculetin boosted the bone morphogenetic protein-2 expression, and alkaline phosphatase activation of differentiating MC3T3-E1 cells. The presence of aesculetin strengthened the expression of collagen type 1 and osteoprotegerin and transcription of Runt-related transcription factor 2 in differentiating osteoblasts for 9 days. When ≥1-5 μM aesculetin was added to differentiating cells for 15-18 days, the induction of non-collagenous proteins of bone sialoprotein II, osteopontin, osteocalcin, and osteonectin was markedly enhanced, facilitating the formation of hydroxyapatite crystals and mineralized collagen matrix. The induction of annexin V and PHOSPHO 1 was further augmented in ≥5 μM aesculetin-treated differentiating osteoblasts for 21 days. In addition, the levels of tissue-nonspecific alkaline phosphatase and collagen type 1 were further enhanced within the extracellular space and on matrix vesicles of mature osteoblasts treated with aesculetin, indicating matrix vesicle-mediated bone mineralization. Finally, aesculetin markedly accelerated the production of thrombospondin-1 and tenascin C in mature osteoblasts, leading to their adhesion to preformed collagen matrix. Therefore, aesculetin enhanced osteoblast differentiation, and matrix vesicle biogenesis and mineralization. These findings suggest that aesculetin may be a potential osteo-inductive agent preventing bone pathologies or enhancing bone regeneration.

203. Unravel the Local Complexity of Biological Environments by MALDI Mass Spectrometry Imaging.

Int J Mol Sci

2021 Nov 17

Sgobba E, Daguerre Y, Giampà M.

204. Osr1 Is Required for Mesenchymal Derivatives That Produce Collagen in the Bladder.

Int J Mol Sci

2021 Nov 17

Murugapoopathy V, Cammisotto PG, Mossa AH

205. Genomic Regions Associated with Fusarium Wilt Resistance in Flax.

Int J Mol Sci

2021 Nov 17

Kanapin A, Bankin M, Rozhmina T

206. COX Inhibitory and Cytotoxic Naphthoketal-Bearing Polyketides from Sparticola junci.

Int J Mol Sci

2021 Nov 17

Garcia KYM, Quimque MTJ, Primahana G

207. The Role of Cannabinoids in Bone Metabolism: A New Perspective for Bone Disorders.

Int J Mol Sci

2021 Nov 16

Saponaro F, Ferrisi R, Gado F

208. Auxin Metabolite Profiling in Isolated and Intact Plant Nuclei.

Int J Mol Sci

2021 Nov 16

Skalický V, Vojtková T, Pěnčík A

209. Enigmatic Pilus-Like Endospore Appendages of Bacillus cereus Group Species.

Int J Mol Sci

2021 Nov 16

Zegeye ED, Pradhan B, Llarena AK

210. Diabetes and Cognitive Impairment: A Role for Glucotoxicity and Dopaminergic Dysfunction.

Int J Mol Sci

2021 Nov 16

Pignalosa FC, Desiderio A, Mirra P

211. p130Cas Is Correlated with EREG Expression and a Prognostic Factor Depending on Colorectal Cancer Stage and Localization Reducing FOLFIRI Efficacy.

Int J Mol Sci

2021 Nov 16

Kumbrink J, Li P, Pók-Udvari A

212. Assessment of the Effects of Dietary Vitamin D Levels on Olanzapine-Induced Metabolic Side Effects: Focus on the Endocannabinoidome-Gut Microbiome Axis.

Int J Mol Sci

2021 Nov 16

Abolghasemi A, Manca C, Iannotti FA

213. The Mechanism of Leptin on Inhibiting Fibrosis and Promoting Browning of White Fat by Reducing ITGA5 in Mice.

Int J Mol Sci

2021 Nov 16

Liu Y, Li Y, Liang J

214. The Somatic Mutation Paradigm in Congenital Malformations: Hirschsprung Disease as a Model.

Int J Mol Sci

2021 Nov 16

MacKenzie KC, Garritsen R, Chauhan RK

215. Special Issue "Microglia Heterogeneity and Its Relevance for Translational Research".

Int J Mol Sci

2021 Nov 16

Michelucci A, Miron VE, Priller J.

216. Intramuscular Exposure to a Lethal Dose of Ricin Toxin Leads to Endothelial Glycocalyx Shedding and Microvascular Flow Abnormality in Mice and Swine.

Int J Mol Sci

2021 Nov 16

Sapoznikov A, Gal Y, Evgy Y

217. NTRK Gene Fusion Detection in Atypical Spitz Tumors.

Int J Mol Sci

2021 Nov 15

Cappellesso R, Nozzoli F, Zito Marino F

218. Hyperandrogenism? Increased 17, 20-Lyase Activity? A Metanalysis and Systematic Review of Altered Androgens in Boys and Girls with Autism.

Int J Mol Sci

2021 Nov 15

Gasser BA, Buerki SF, Kurz J

219. Garlic Volatile Diallyl Disulfide Induced Cucumber Resistance to Downy Mildew.

Int J Mol Sci

2021 Nov 15

Yang F, Wang H, Zhi C

220. Nuclear Receptors in Myocardial and Cerebral Ischemia-Mechanisms of Action and Therapeutic Strategies.

Int J Mol Sci

2021 Nov 15

Rzemieniec J, Castiglioni L, Gelosa P

221. To Discover the Efficient and Novel Drug Targets in Human Cancers Using CRISPR/Cas Screening and Databases.

Int J Mol Sci

2021 Nov 15

Onishi I, Yamamoto K, Kinowaki Y

Int J Mol Sci

2021 Nov 15

Xu X, Zou X.

223. Efficacy of Cord Blood Cell Therapy for Hutchinson-Gilford Progeria Syndrome-A Case Report.

Int J Mol Sci

2021 Nov 15

Suh MR, Lim I, Kim J

224. Chymase as a Novel Therapeutic Target in Acute Pancreatitis.

Int J Mol Sci

2021 Nov 15

Kuramoto T, Jin D, Komeda K

225. Incretins in the Therapy of Diabetic Kidney Disease.

Int J Mol Sci

2021 Nov 15

Przezak A, Bielka W, Pawlik A.

226. Molecular Evolution of Calcium Signaling and Transport in Plant Adaptation to Abiotic Stress.

Int J Mol Sci

2021 Nov 15

Tong T, Li Q, Jiang W

227. Inhibitory Potential of New Phenolic Hydrazide-Hydrazones with a Decoy Substrate Fragment towards Laccase from a Phytopathogenic Fungus: SAR and Molecular Docking Studies.

Int J Mol Sci

2021 Nov 14

Maniak H, Talma M, Giurg M.

228. Small Reduced Graphene Oxides for Highly Efficient Oxygen Reduction Catalysts.

Int J Mol Sci

2021 Nov 14

Bak SJ, Kim SI, Lim SY

229. The Role of Ancestral Duplicated Genes in Adaptation to Growth on Lactate, a Non-Fermentable Carbon Source for the Yeast Saccharomyces cerevisiae.

Int J Mol Sci

2021 Nov 14

Mattenberger F, Fares MA, Toft C

230. Bridging Radiotherapy to Immunotherapy: The IFN-JAK-STAT Axis.

Int J Mol Sci

2021 Nov 14

Shi LZ, Bonner JA.

231. Metal Sulfide Semiconductor Nanomaterials and Polymer Microgels for Biomedical Applications.

Int J Mol Sci

2021 Nov 14

Paca AM, Ajibade PA.

232. Extracellular Vesicles Physiological Role and the Particular Case of Disease-Spreading Mechanisms in Polyglutamine Diseases.

Int J Mol Sci

2021 Nov 13

Moreira R, Mendonça LS, Pereira de Almeida L.

233. Ablation of Red Stable Transfected Claudin Expressing Canine Prostate Adenocarcinoma and Transitional Cell Carcinoma Cell Lines by C-CPE Gold-Nanoparticle-Mediated Laser Intervention.

Int J Mol Sci

2021 Nov 13

Alnajjar S, Nolte I, Becker A

234. Treatment of HT29 Human Colorectal Cancer Cell Line with Nanocarrier-Encapsulated Camptothecin Reveals Histone Modifier Genes in the Wnt Signaling Pathway as Important Molecular Cues for Colon Cancer Targeting.

Int J Mol Sci

2021 Nov 13

Farhana A, Koh AE, Kothandan S

Cancer cells are able to proliferate in an unregulated manner. There are several mechanisms involved that propel such neoplastic transformations. One of these processes involves bypassing cell death through changes in gene expression and, consequently, cell growth. This involves a complex epigenetic interaction within the cell, which drives it towards oncogenic transformations. These epigenetic events augment cellular growth by potentially altering chromatin structures and influencing key gene expressions. Therapeutic mechanisms have been developed to combat this by taking advantage of the underlying oncogenic mechanisms through chemical modulation. Camptothecin (CPT) is an example of this type of drug. It is a selective topoisomerase I inhibitor that is effective against many cancers, such as colorectal cancer. Previously, we successfully formulated a magnetic nanocarrier-conjugated CPT with β-cyclodextrin and iron NPs (Fe3O4) cross-linked using EDTA (CPT-CEF). Compared to CPT alone, it boasts higher efficacy due to its selective targeting and increased solubility. In this study, we treated HT29 colon cancer cells with CPT-CEF and attempted to investigate the cytotoxic effects of the formulation through an epigenetic perspective. By using RNA-Seq, several differentially expressed genes were obtained (p < 0.05). Enrichr was then used for the over-representation analysis, and the genes were compared to the epigenetic roadmap and histone modification database. The results showed that the DEGs had a high correlation with epigenetic modifications involving histone H3 acetylation. Furthermore, a subset of these genes was shown to be associated with the Wnt/β-catenin signaling pathway, which is highly upregulated in a large number of cancer cells. These genes could be investigated as downstream therapeutic targets against the uncontrolled proliferation of cancer cells. Further interaction analysis of the identified genes with the key genes of the Wnt/β-catenin signaling pathway in colorectal cancer identified the direct interactors and a few transcription regulators. Further analysis in cBioPortal confirmed their genetic alterations and their distribution across patient samples. Thus, the findings of this study reveal that colorectal cancer could be reversed by treatment with the CPT-CEF nanoparticle-conjugated nanocarrier through an epigenetic mechanism.

235. In-Depth Sequence Analysis of Bread Wheat VRN1 Genes.

Int J Mol Sci

2021 Nov 13

Strejčková B, Milec Z, Holušová K

236. Secretome Analysis of Rabbit and Human Mesenchymal Stem and Endothelial Progenitor Cells: A Comparative Study.

Int J Mol Sci

2021 Nov 13

Vašíček J, Baláži A, Tirpáková M

237. Epigenetic Mechanisms in Memory and Cognitive Decline Associated with Aging and Alzheimer's Disease.

Int J Mol Sci

2021 Nov 13

Maity S, Farrell K, Navabpour S

238. Encapsulin Based Self-Assembling Iron-Containing Protein Nanoparticles for Stem Cells MRI Visualization.

Int J Mol Sci

2021 Nov 12

Gabashvili AN, Vodopyanov SS, Chmelyuk NS

239. Control of Chromatin Organization and Chromosome Behavior during the Cell Cycle through Phase Separation.

Int J Mol Sci

2021 Nov 12

Li J, Gao J, Wang R.

240. Mold, Mycotoxins and a Dysregulated Immune System: A Combination of Concern?

Int J Mol Sci

2021 Nov 12

Kraft S, Buchenauer L, Polte T.

241. Therapeutic Effects of Inhibitor of ompA Expression against Carbapenem-Resistant Acinetobacter baumannii Strains.

Int J Mol Sci

2021 Nov 12

Na SH, Jeon H, Oh MH

242. Maturation of UTR-Derived sRNAs Is Modulated during Adaptation to Different Growth Conditions.

Int J Mol Sci

2021 Nov 12

Spanka DT, Klug G.

243. Enhanced Muscle Strength in Dyslipidemic Mice and Its Relation to Increased Capacity for Fatty Acid Oxidation.

Int J Mol Sci

2021 Nov 12

Tomczyk M, Braczko A, Jablonska P

244. Bioconversion of Lignocellulosic Biomass into Value Added Products under Anaerobic Conditions: Insight into Proteomic Studies.

Int J Mol Sci

2021 Nov 12

Vélez-Mercado MI, Talavera-Caro AG, Escobedo-Uribe KM

245. Safety of Surgery after Neoadjuvant Targeted Therapies in Non-Small Cell Lung Cancer: A Narrative Review.

Int J Mol Sci

2021 Nov 12

Marjanski T, Dziedzic R, Kowalczyk A

246. Optimizing the Aspect Ratio of Nanopatterned Mesoporous TiO(2) Thin-Film Layer to Improve Energy Conversion Efficiency of Perovskite Solar Cells.

Int J Mol Sci

2021 Nov 12

Yang HY, Chuquer A, Han SH

247. Genome-Wide Characterization of Salt-Responsive miRNAs, circRNAs and Associated ceRNA Networks in Tomatoes.

Int J Mol Sci

2021 Nov 12

Wang Z, Li N, Yu Q

248. CTI-2 Inhibits Metastasis and Epithelial-Mesenchymal Transition of Breast Cancer Cells by Modulating MAPK Signaling Pathway.

Int J Mol Sci

2021 Nov 12

Ke J, Han W, Meng F

249. Signaling at Physical Barriers during Pollen-Pistil Interactions.

Int J Mol Sci

2021 Nov 12

Robichaux KJ, Wallace IS.

250. (Un)expected Similarity of the Temporary Adhesive Systems of Marine, Brackish, and Freshwater Flatworms.

Int J Mol Sci

2021 Nov 12

Bertemes P, Pjeta R, Wunderer J

251. The Epithelial-to-Mesenchymal Transition (EMT) in the Development and Metastasis of Malignant Pleural Mesothelioma.

Int J Mol Sci

2021 Nov 11

Ramundo V, Zanirato G, Aldieri E.

252. Multifunctional Cyanine-Based Theranostic Probe for Cancer Imaging and Therapy.

Int J Mol Sci

2021 Nov 11

Peng CL, Shih YH, Chiang PF

253. Eco-Friendly Ether and Ester-Urethane Prepolymer: Structure, Processing and Properties.

Int J Mol Sci

2021 Nov 11

Niesiobędzka J, Głowińska E, Datta J.

254. Investigation of Ifosfamide Toxicity Induces Common Upstream Regulator in Liver and Kidney.

Int J Mol Sci

2021 Nov 11

Han HY, Choi MS, Yoon S

255. 3D Cell Culture Systems: Tumor Application, Advantages, and Disadvantages.

Int J Mol Sci

2021 Nov 11

Habanjar O, Diab-Assaf M, Caldefie-Chezet F

The traditional two-dimensional (2D) in vitro cell culture system (on a flat support) has long been used in cancer research. However, this system cannot be fully translated into clinical trials to ideally represent physiological conditions. This culture cannot mimic the natural tumor microenvironment due to the lack of cellular communication (cell-cell) and interaction (cell-cell and cell-matrix). To overcome these limitations, three-dimensional (3D) culture systems are increasingly developed in research and have become essential for tumor research, tissue engineering, and basic biology research. 3D culture has received much attention in the field of biomedicine due to its ability to mimic tissue structure and function. The 3D matrix presents a highly dynamic framework where its components are deposited, degraded, or modified to delineate functions and provide a platform where cells attach to perform their specific functions, including adhesion, proliferation, communication, and apoptosis. So far, various types of models belong to this culture: either the culture based on natural or synthetic adherent matrices used to design 3D scaffolds as biomaterials to form a 3D matrix or based on non-adherent and/or matrix-free matrices to form the spheroids. In this review, we first summarize a comparison between 2D and 3D cultures. Then, we focus on the different components of the natural extracellular matrix that can be used as supports in 3D culture. Then we detail different types of natural supports such as matrigel, hydrogels, hard supports, and different synthetic strategies of 3D matrices such as lyophilization, electrospiding, stereolithography, microfluid by citing the advantages and disadvantages of each of them. Finally, we summarize the different methods of generating normal and tumor spheroids, citing their respective advantages and disadvantages in order to obtain an ideal 3D model (matrix) that retains the following characteristics: better biocompatibility, good mechanical properties corresponding to the tumor tissue, degradability, controllable microstructure and chemical components like the tumor tissue, favorable nutrient exchange and easy separation of the cells from the matrix.

256. The Effect of Tuberculosis Antimicrobials on the Immunometabolic Profiles of Primary Human Macrophages Stimulated with Mycobacterium tuberculosis.

Int J Mol Sci

2021 Nov 10

Cahill C, Cox DJ, O'Connell F

Tuberculosis (TB) remains a global health challenge. Patients with drug-sensitive and drug-resistant TB undergo long, arduous, and complex treatment regimens, often involving multiple antimicrobials. While these drugs were initially implemented based on their bactericidal effects, some studies show that TB antimicrobials can also directly affect cells of the immune system, altering their immune function. As use of these antimicrobials has been the mainstay of TB therapy for over fifty years now, it is more important than ever to understand how these antimicrobials affect key pathways of the immune system. One such central pathway, which underpins the immune response to a variety of infections, is immunometabolism, namely glycolysis and oxidative phosphorylation (OXPHOS). We hypothesise that in addition to their direct bactericidal effect on Mycobacterium tuberculosis (Mtb), current TB antimicrobials can modulate immunometabolic profiles and alter mitochondrial function in primary human macrophages. Human monocyte-derived macrophages (hMDMs) were differentiated from PBMCs isolated from healthy blood donors, and treated with four first-line and six second-line TB antimicrobials three hours post stimulation with either iH37Rv-Mtb or lipopolysaccharide (LPS). 24 h post stimulation, baseline metabolism and mitochondrial function were determined using the Seahorse Extracellular Flux Analyser. The effect of these antimicrobials on cytokine and chemokine production was also assayed using Meso Scale Discovery Multi-Array technology. We show that some of the TB antimicrobials tested can significantly alter OXPHOS and glycolysis in uninfected, iH37Rv-Mtb, and LPS-stimulated hMDMs. We also demonstrate how these antimicrobial-induced immunometabolic effects are linked with alterations in mitochondrial function. Our results show that TB antimicrobials, specifically clofazimine, can modify host immunometabolism and mitochondrial function. Moreover, clofazimine significantly increased the production of IL-6 in human macrophages that were stimulated with iH37Rv-Mtb. This provides further insight into the use of some of these TB antimicrobials as potential host-directed therapies in patients with early and active disease, which could help to inform TB treatment strategies in the future.

257. The Role of Nucleocytoplasmic Transport Defects in Amyotrophic Lateral Sclerosis.

Int J Mol Sci

2021 Nov 10

Vanneste J, Van Den Bosch L.

258. Patient-Specific iPSC-Derived Neural Differentiated and Hepatocyte-like Cells, Carrying the Compound Heterozygous Mutation p.V1023Sfs*15/p.G992R, Present the "Variant" Biochemical Phenotype of Niemann-Pick Type C1 Disease.

Int J Mol Sci

2021 Nov 10

Völkner C, Liedtke M, Untucht R

259. Vascular Polyurethane Prostheses Modified with a Bioactive Coating-Physicochemical, Mechanical and Biological Properties.

Int J Mol Sci

2021 Nov 10

Kuźmińska A, Wojciechowska A, Butruk-Raszeja BA.

260. Molecular Mechanisms and Cellular Contribution from Lung Fibrosis to Lung Cancer Development.

Int J Mol Sci

2021 Nov 10

Samarelli AV, Masciale V, Aramini B

261. Zinc Induced Aβ(16) Aggregation Modeled by Molecular Dynamics.

Int J Mol Sci

2021 Nov 10

Tolstova AP, Makarov AA, Adzhubei AA.

262. The Fusion of CLEC12A and MIR223HG Arises from a trans-Splicing Event in Normal and Transformed Human Cells.

Int J Mol Sci

2021 Nov 10

Dhungel BP, Monteuuis G, Giardina C

263. Delivery of Nitric Oxide in the Cardiovascular System: Implications for Clinical Diagnosis and Therapy.

Int J Mol Sci

2021 Nov 10

Ma T, Zhang Z, Chen Y

264. There Is Strength in Numbers: Quantitation of Fc Gamma Receptors on Murine Tissue-Resident Macrophages.

Int J Mol Sci

2021 Nov 10

Vorsatz C, Friedrich N, Nimmerjahn F

265. Structural Basis for the Functional Diversity of Centrins: A Focus on Calcium Sensing Properties and Target Recognition.

Int J Mol Sci

2021 Nov 10

Pedretti M, Bombardi L, Conter C

266. Micro-RNA Implications in Type-1 Diabetes Mellitus: A Review of Literature.

Int J Mol Sci

2021 Nov 10

Margaritis K, Margioula-Siarkou G, Giza S

267. Chemoattraction of Neoplastic Glial Cells with CXCL10, CCL2 and CCL11 as a Paradigm for a Promising Therapeutic Approach for Primary Brain Tumors.

Int J Mol Sci

2021 Nov 10

Déry L, Charest G, Guérin B

268. The Emerging Role of Presepsin (P-SEP) in the Diagnosis of Sepsis in the Critically Ill Infant: A Literature Review.

Int J Mol Sci

2021 Nov 10

Maddaloni C, De Rose DU, Santisi A

269. Synthesis of Porous Polydimethylsiloxane Gold Nanoparticles Composites by a Single Step Laser Ablation Process.

Int J Mol Sci

2021 Nov 10

Cutroneo M, Havranek V, Mackova A

270. Fukutin Protein Participates in Cell Proliferation by Enhancing Cyclin D1 Expression through Binding to the Transcription Factor Activator Protein-1: An In Vitro Study.

Int J Mol Sci

2021 Nov 10

Okamura Y, Yamamoto T, Tsukui R

271. Transcriptional Regulation of Ripening in Chili Pepper Fruits (Capsicum spp.).

Int J Mol Sci

2021 Nov 10

Villa-Rivera MG, Ochoa-Alejo N.

272. Profiling the Proteome of Cyst Nematode-Induced Syncytia on Tomato Roots.

Int J Mol Sci

2021 Nov 10

Filipecki M, Żurczak M, Matuszkiewicz M

273. Recent Trends in Cancer Genomics and Bioinformatics Tools Development.

Int J Mol Sci

2021 Nov 10

Anashkina AA, Leberfarb EY, Orlov YL.

274. Towards the Idea of Molecular Brains.

Int J Mol Sci

2021 Nov 1

Timsit Y, Grégoire SP.

How can single cells without nervous systems perform complex behaviours such as habituation, associative learning and decision making, which are considered the hallmark of animals with a brain? Are there molecular systems that underlie cognitive properties equivalent to those of the brain? This review follows the development of the idea of molecular brains from Darwin's "root brain hypothesis", through bacterial chemotaxis, to the recent discovery of neuron-like r-protein networks in the ribosome. By combining a structural biology view with a Bayesian brain approach, this review explores the evolutionary labyrinth of information processing systems across scales. Ribosomal protein networks open a window into what were probably the earliest signalling systems to emerge before the radiation of the three kingdoms. While ribosomal networks are characterised by long-lasting interactions between their protein nodes, cell signalling networks are essentially based on transient interactions. As a corollary, while signals propagated in persistent networks may be ephemeral, networks whose interactions are transient constrain signals diffusing into the cytoplasm to be durable in time, such as post-translational modifications of proteins or second messenger synthesis. The duration and nature of the signals, in turn, implies different mechanisms for the integration of multiple signals and decision making. Evolution then reinvented networks with persistent interactions with the development of nervous systems in metazoans. Ribosomal protein networks and simple nervous systems display architectural and functional analogies whose comparison could suggest scale invariance in information processing. At the molecular level, the significant complexification of eukaryotic ribosomal protein networks is associated with a burst in the acquisition of new conserved aromatic amino acids. Knowing that aromatic residues play a critical role in allosteric receptors and channels, this observation suggests a general role of π systems and their interactions with charged amino acids in multiple signal integration and information processing. We think that these findings may provide the molecular basis for designing future computers with organic processors.

275. The Clinical Application of Pulsed Radiofrequency Induces Inflammatory Pain via MAPKs Activation: A Novel Hint for Pulsed Radiofrequency Treatment.

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